A monoclonal antibody to ameliorate central nervous system infection and improve survival in a murine model of human Enterovirus-A71 encephalomyelitis
Background: Enterovirus A71 (EV-A71) encephalomyelitis is an often fatal disease for which there is no specific treatment available. Passive immunization with a specific monoclonal antibody to EV-A71 was used on a murine model of EV-A71 encephalomyelitis to evaluate its therapeutic effectiveness b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier B.V.
2016
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/12648/ http://ir.unimas.my/id/eprint/12648/1/A%20monoclonal%20antibody%20to%20ameliorate%20central%20nervous%20system%20infection%20%28abstract%29.pdf |
| Summary: | Background: Enterovirus A71 (EV-A71) encephalomyelitis is an often fatal disease for which there is no
specific treatment available. Passive immunization with a specific monoclonal antibody to EV-A71 was
used on a murine model of EV-A71 encephalomyelitis to evaluate its therapeutic effectiveness before and
after established central nervous system (CNS) infection.
Methods: Mice were intraperitoneally-infected with a mouse-adapted EV-A71 strain and treated with a
dose of monoclonal antibody (MAb) daily for 3 days on day 1, 2 and 3 post-infection or for 3 days on 3, 4
and 5 post-infection. Treatment effectiveness was evaluated by signs of infection and survival rate.
Histopathology and qPCR analyses were performed on mice sacrificed a day after completing treatment.
Results: In mock-treated mice, CNS infection was established from day 3 post-infection. All mice treated
before established CNS infection, survived and recovered completely without CNS infection. All mice
treated after established CNS infection survived with mild paralysis, and viral load and antigens/RNA at
day 6 post-infection were significantly reduced.
Conclusions: Passive immunization with our MAb could prevent CNS infection in mice if given early
before the establishment of CNS infection. It could also ameliorate established CNS infection if optimal
and repeated doses were given. |
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