Construction of an induccible vector containing marker gene

This study concerns on understanding the function of inducible promoter that can controlled the expression in plant. The used of controllable promoter region permit a regulated of a foreign gene in plant. The cassette contain the induce promoter carry a maker gene (GUS and LUC) was constructed in pU...

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Bibliographic Details
Main Author: Nurul Ashikin, Mohamed Ali
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2005
Subjects:
Online Access:http://ir.unimas.my/id/eprint/1261/
http://ir.unimas.my/id/eprint/1261/3/Nurul%20Ashikin.pdf
Description
Summary:This study concerns on understanding the function of inducible promoter that can controlled the expression in plant. The used of controllable promoter region permit a regulated of a foreign gene in plant. The cassette contain the induce promoter carry a maker gene (GUS and LUC) was constructed in pUC plasmid. This construct based on the alcR regulatOlY system from the fungus Aspergilus nidulans with the application of ethanol as inducer.