Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces
This study aimed to determine the biofilm formation ability by Salmonella Typhi on cucumber, mango and guava surface, as well as to determine the relationship between time contact and biofilm formation. Crystal violet assay was performed to quantify the biofilm formation based on the value of opti...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
UPM
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/12225/ http://ir.unimas.my/id/eprint/12225/1/Noorlis.pdf |
| _version_ | 1848837152517914624 |
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| author | Tang, P. L., C. F, Pui, Wong,, W. C Noorlis,, A. Son, R. |
| author_facet | Tang, P. L., C. F, Pui, Wong,, W. C Noorlis,, A. Son, R. |
| author_sort | Tang, P. L., |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | This study aimed to determine the biofilm formation ability by Salmonella Typhi on cucumber,
mango and guava surface, as well as to determine the relationship between time contact and biofilm formation.
Crystal violet assay was performed to quantify the biofilm formation based on the value of optical density at
570 nm of the destaining crystal violet at the specific interval time. The result showed that the attachment of the
bacterial cells on the fresh produce surface increased with the contact time. The readings of OD570 at time 12 h
for cucumber, mango and guava surfaces were 0.824, 0.683 and 0.598, respectively, indicating that the biofilm
formation by Salmonella Typhi on different fresh produce surface varied with time. Since the result showed
that Salmonella Typhi formed biofilm on fresh produce surfaces, hygienic practice from farm to fork including
handling, processing, distribution and storage of the fresh produce should be of concern. |
| first_indexed | 2025-11-15T06:35:07Z |
| format | Article |
| id | unimas-12225 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:35:07Z |
| publishDate | 2012 |
| publisher | UPM |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-122252021-07-01T14:15:51Z http://ir.unimas.my/id/eprint/12225/ Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces Tang, P. L., C. F, Pui, Wong,, W. C Noorlis,, A. Son, R. SB Plant culture This study aimed to determine the biofilm formation ability by Salmonella Typhi on cucumber, mango and guava surface, as well as to determine the relationship between time contact and biofilm formation. Crystal violet assay was performed to quantify the biofilm formation based on the value of optical density at 570 nm of the destaining crystal violet at the specific interval time. The result showed that the attachment of the bacterial cells on the fresh produce surface increased with the contact time. The readings of OD570 at time 12 h for cucumber, mango and guava surfaces were 0.824, 0.683 and 0.598, respectively, indicating that the biofilm formation by Salmonella Typhi on different fresh produce surface varied with time. Since the result showed that Salmonella Typhi formed biofilm on fresh produce surfaces, hygienic practice from farm to fork including handling, processing, distribution and storage of the fresh produce should be of concern. UPM 2012 Article PeerReviewed text en http://ir.unimas.my/id/eprint/12225/1/Noorlis.pdf Tang, P. L., and C. F, Pui, and Wong,, W. C and Noorlis,, A. and Son, R. (2012) Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces. International Food Research Journal, 19 (1). pp. 71-76. ISSN 19854668 http://www.ifrj.upm.edu.my/ |
| spellingShingle | SB Plant culture Tang, P. L., C. F, Pui, Wong,, W. C Noorlis,, A. Son, R. Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_full | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_fullStr | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_full_unstemmed | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_short | Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces |
| title_sort | biofilm forming ability and time course study of growth of salmonella typhi on fresh produce surfaces |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/12225/ http://ir.unimas.my/id/eprint/12225/ http://ir.unimas.my/id/eprint/12225/1/Noorlis.pdf |