Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant
Anaerobic ammonium oxidation (anammox) process plays an important role in biological nitrogen removal. The bacteria catalyzing anammox process belongs to phylum Planctomycetales. The objective of this research is to characterize the anammox bacteria from leachate sample collected from a local wastew...
| Main Author: | |
|---|---|
| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2011
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/1209/ http://ir.unimas.my/id/eprint/1209/1/Characterization%20of%20anaerobic%20ammonium%20oxidation%20%28Anammox%29%20bacteria%20%2824%20pages%29.pdf http://ir.unimas.my/id/eprint/1209/9/Characterization%20of%20anaerobic%20ammonium%20oxidation%20%28Anammox%29%20bacteria%20%28fulltext%29.pdf |
| _version_ | 1848834712737415168 |
|---|---|
| author | Chandrasuriya, Lingam |
| author_facet | Chandrasuriya, Lingam |
| author_sort | Chandrasuriya, Lingam |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Anaerobic ammonium oxidation (anammox) process plays an important role in biological nitrogen removal. The bacteria catalyzing anammox process belongs to phylum Planctomycetales. The objective of this research is to characterize the anammox bacteria from leachate sample collected from a local wastewater treatment plant. Characterization of anammox bacteria includes enrichment, morphology, physico-chemical and molecular analysis. Screening for anammox bacteria was conducted using both enrichment culture and directly from leachate sample. Identification of anammox bacteria from enrichment culture and leachate sample using molecular method involves DNA extraction and PCR. Presence of anammox activity in enrichment culture was detected using physico-chemical method. No bands were obtained in peR using DNA extracted from enrichment culture. Presence of uncultured Planctomycete bacteria with potential anammox activity was detected through molecular analysis directly in leachate sample. Identification of anammox bacteria are advantageous as it can be applied in wastewater treatment plants to the level of ammonium and enhance the quality ofdischarged effluent. |
| first_indexed | 2025-11-15T05:56:20Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-1209 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T05:56:20Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-12092023-02-01T09:03:08Z http://ir.unimas.my/id/eprint/1209/ Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant Chandrasuriya, Lingam QR Microbiology Anaerobic ammonium oxidation (anammox) process plays an important role in biological nitrogen removal. The bacteria catalyzing anammox process belongs to phylum Planctomycetales. The objective of this research is to characterize the anammox bacteria from leachate sample collected from a local wastewater treatment plant. Characterization of anammox bacteria includes enrichment, morphology, physico-chemical and molecular analysis. Screening for anammox bacteria was conducted using both enrichment culture and directly from leachate sample. Identification of anammox bacteria from enrichment culture and leachate sample using molecular method involves DNA extraction and PCR. Presence of anammox activity in enrichment culture was detected using physico-chemical method. No bands were obtained in peR using DNA extracted from enrichment culture. Presence of uncultured Planctomycete bacteria with potential anammox activity was detected through molecular analysis directly in leachate sample. Identification of anammox bacteria are advantageous as it can be applied in wastewater treatment plants to the level of ammonium and enhance the quality ofdischarged effluent. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/1209/1/Characterization%20of%20anaerobic%20ammonium%20oxidation%20%28Anammox%29%20bacteria%20%2824%20pages%29.pdf text en http://ir.unimas.my/id/eprint/1209/9/Characterization%20of%20anaerobic%20ammonium%20oxidation%20%28Anammox%29%20bacteria%20%28fulltext%29.pdf Chandrasuriya, Lingam (2011) Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant. [Final Year Project Report / IMRAD] |
| spellingShingle | QR Microbiology Chandrasuriya, Lingam Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant |
| title | Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant |
| title_full | Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant |
| title_fullStr | Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant |
| title_full_unstemmed | Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant |
| title_short | Characterization of anaerobic ammonium oxidation (Anammox) bacteria from local wastewater treatment plant |
| title_sort | characterization of anaerobic ammonium oxidation (anammox) bacteria from local wastewater treatment plant |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/1209/ http://ir.unimas.my/id/eprint/1209/1/Characterization%20of%20anaerobic%20ammonium%20oxidation%20%28Anammox%29%20bacteria%20%2824%20pages%29.pdf http://ir.unimas.my/id/eprint/1209/9/Characterization%20of%20anaerobic%20ammonium%20oxidation%20%28Anammox%29%20bacteria%20%28fulltext%29.pdf |