The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer
Objective: To investigate the allele and genotype frequencies of NFKB1 −94 ins/del ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier Ltd.
2013
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/11981/ http://ir.unimas.my/id/eprint/11981/1/The%20functional%E2%88%92%2094%20insertion%20deletion%20ATTG%20polymorphism%20in%20the%20promoter%20region%20of%20NFKB1%20%28abstract%29.pdf |
| _version_ | 1848837101716504576 |
|---|---|
| author | Mohd Shafi’i, Mohd Suzairi Tan, Shing Cheng Abdul Aziz, Ahmad Aizat Mustapha, Mohd Aminudin Siti Nurfatimah, Mohd Shahpudin Zakaria Dzulkarnaen, Andee Ravindran, Ankathil |
| author_facet | Mohd Shafi’i, Mohd Suzairi Tan, Shing Cheng Abdul Aziz, Ahmad Aizat Mustapha, Mohd Aminudin Siti Nurfatimah, Mohd Shahpudin Zakaria Dzulkarnaen, Andee Ravindran, Ankathil |
| author_sort | Mohd Shafi’i, Mohd Suzairi |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Objective: To investigate the allele and genotype frequencies of NFKB1 −94 ins/del ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of 474 study subjects, which consisted of 237 histopathologically confirmed CRC patients and an equal number of cancer-free controls. The NFKB1 −94 ins/del ATTG (rs28720239) polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and confirmed by DNA sequencing. The association between the polymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95% confidence intervals (CIs) using unconditional logistic regression analysis. Results: The frequencies of wildtype (del/del), heterozygous (del/ins) and variant (ins/ins) genotypes in CRC patients were 31.7%, 53.6% and 14.8%, respectively, while those in cancer-free controls were 35.0%, 58.2% and 6.8%, respectively. The frequency of the variant genotype was significantly higher in cases compared to controls (P < 0.01). Evaluation of the risk association of the polymorphic genotypes revealed that the variant genotype could contribute to a significantly increased risk of CRC (OR = 2.42, 95% CI = 1.24–4.73, P < 0.01). Conclusions: The variant allele of NFKB1 −94 ins/del ATTG (rs28362491) polymorphism is associated with higher risk of sporadic CRC in Malaysian population. |
| first_indexed | 2025-11-15T06:34:19Z |
| format | Article |
| id | unimas-11981 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:34:19Z |
| publishDate | 2013 |
| publisher | Elsevier Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-119812023-05-29T01:10:43Z http://ir.unimas.my/id/eprint/11981/ The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer Mohd Shafi’i, Mohd Suzairi Tan, Shing Cheng Abdul Aziz, Ahmad Aizat Mustapha, Mohd Aminudin Siti Nurfatimah, Mohd Shahpudin Zakaria Dzulkarnaen, Andee Ravindran, Ankathil RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) Objective: To investigate the allele and genotype frequencies of NFKB1 −94 ins/del ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population. Methods: Genomic DNA was extracted from the peripheral blood samples of 474 study subjects, which consisted of 237 histopathologically confirmed CRC patients and an equal number of cancer-free controls. The NFKB1 −94 ins/del ATTG (rs28720239) polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and confirmed by DNA sequencing. The association between the polymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95% confidence intervals (CIs) using unconditional logistic regression analysis. Results: The frequencies of wildtype (del/del), heterozygous (del/ins) and variant (ins/ins) genotypes in CRC patients were 31.7%, 53.6% and 14.8%, respectively, while those in cancer-free controls were 35.0%, 58.2% and 6.8%, respectively. The frequency of the variant genotype was significantly higher in cases compared to controls (P < 0.01). Evaluation of the risk association of the polymorphic genotypes revealed that the variant genotype could contribute to a significantly increased risk of CRC (OR = 2.42, 95% CI = 1.24–4.73, P < 0.01). Conclusions: The variant allele of NFKB1 −94 ins/del ATTG (rs28362491) polymorphism is associated with higher risk of sporadic CRC in Malaysian population. Elsevier Ltd. 2013 Article PeerReviewed text en http://ir.unimas.my/id/eprint/11981/1/The%20functional%E2%88%92%2094%20insertion%20deletion%20ATTG%20polymorphism%20in%20the%20promoter%20region%20of%20NFKB1%20%28abstract%29.pdf Mohd Shafi’i, Mohd Suzairi and Tan, Shing Cheng and Abdul Aziz, Ahmad Aizat and Mustapha, Mohd Aminudin and Siti Nurfatimah, Mohd Shahpudin and Zakaria Dzulkarnaen, Andee and Ravindran, Ankathil (2013) The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer. Cancer Epidemiology, 37. pp. 634-638. ISSN 1877-7821 http://www.sciencedirect.com/science/article/pii/S1877782113000945 http://dx.doi.org/10.1016/j.canep.2013.05.007 |
| spellingShingle | RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mohd Shafi’i, Mohd Suzairi Tan, Shing Cheng Abdul Aziz, Ahmad Aizat Mustapha, Mohd Aminudin Siti Nurfatimah, Mohd Shahpudin Zakaria Dzulkarnaen, Andee Ravindran, Ankathil The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer |
| title | The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer |
| title_full | The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer |
| title_fullStr | The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer |
| title_full_unstemmed | The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer |
| title_short | The functional −94 insertion/deletion ATTG polymorphism in the promoter region of NFKB1 gene increases the risk of sporadic colorectal cancer |
| title_sort | functional −94 insertion/deletion attg polymorphism in the promoter region of nfkb1 gene increases the risk of sporadic colorectal cancer |
| topic | RC Internal medicine RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://ir.unimas.my/id/eprint/11981/ http://ir.unimas.my/id/eprint/11981/ http://ir.unimas.my/id/eprint/11981/ http://ir.unimas.my/id/eprint/11981/1/The%20functional%E2%88%92%2094%20insertion%20deletion%20ATTG%20polymorphism%20in%20the%20promoter%20region%20of%20NFKB1%20%28abstract%29.pdf |