Gender-specific association of NFKBIA promoter polymorphisms with the risk of sporadic colorectal cancer

The inhibitory protein IκBα, encoded by the NFKBIA gene, plays an important role in regulating the activity of nuclear factor-kappa B, a transcription factor which has been implicated in the initiation and progression of cancers. This study aimed to evaluate the association of NFKBIA −826C>T (rs2...

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Main Authors: Tan, Shing Cheng, Mohd Shafi’i, Mohd Suzairi, Abdul Aziz, Ahmad Aizat, Mustapha, Mohd Aminudin, Siti Nurfatimah, Mohd Shahpudin, Venkata Murali, Krishna Bhavaraju, Biswal, Biswal Mohan, Ravindran, Ankathil
Format: Article
Language:English
Published: Springer US 2013
Subjects:
Online Access:http://ir.unimas.my/id/eprint/11980/
http://ir.unimas.my/id/eprint/11980/1/Gender-specific%20association%20of%20NFKBIA%20promoter%20polymorphisms%20%28abstract%29.pdf
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author Tan, Shing Cheng
Mohd Shafi’i, Mohd Suzairi
Abdul Aziz, Ahmad Aizat
Mustapha, Mohd Aminudin
Siti Nurfatimah, Mohd Shahpudin
Venkata Murali, Krishna Bhavaraju
Biswal, Biswal Mohan
Ravindran, Ankathil
author_facet Tan, Shing Cheng
Mohd Shafi’i, Mohd Suzairi
Abdul Aziz, Ahmad Aizat
Mustapha, Mohd Aminudin
Siti Nurfatimah, Mohd Shahpudin
Venkata Murali, Krishna Bhavaraju
Biswal, Biswal Mohan
Ravindran, Ankathil
author_sort Tan, Shing Cheng
building UNIMAS Institutional Repository
collection Online Access
description The inhibitory protein IκBα, encoded by the NFKBIA gene, plays an important role in regulating the activity of nuclear factor-kappa B, a transcription factor which has been implicated in the initiation and progression of cancers. This study aimed to evaluate the association of NFKBIA −826C>T (rs2233406) and −881A>G (rs3138053) polymorphisms with the risk of sporadic colorectal cancer (CRC) in Malaysian population. A case–control study comprising 474 subjects (237 CRC patients and 237 cancer-free controls) was carried out. The polymorphisms were genotyped from the genomic DNA of the study subjects employing PCR–RFLP, followed by DNA sequencing. The association between the polymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95 % confidence intervals (CIs) using unconditional logistic regression analysis. The two polymorphisms were in complete and perfect linkage disequilibrium (D′ = 1.0, r 2 = 1.0). Overall, no statistically significant CRC risk association was found for the polymorphisms (P > 0.05). A similar lack of association was observed when the data were stratified according to ethnicity (P > 0.05). However, stratification by gender revealed a significant inverse association between the heterozygous genotype of the polymorphisms and the risk of CRC among females (OR 0.53, 95 % CI 0.29–0.97, P = 0.04), but not among males (P > 0.05). In conclusion, the heterozygous genotype of the polymorphisms could contribute to a significantly decreased CRC risk among females, but not males, in the Malaysian population.
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institution Universiti Malaysia Sarawak
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recordtype eprints
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spelling unimas-119802023-05-16T03:02:26Z http://ir.unimas.my/id/eprint/11980/ Gender-specific association of NFKBIA promoter polymorphisms with the risk of sporadic colorectal cancer Tan, Shing Cheng Mohd Shafi’i, Mohd Suzairi Abdul Aziz, Ahmad Aizat Mustapha, Mohd Aminudin Siti Nurfatimah, Mohd Shahpudin Venkata Murali, Krishna Bhavaraju Biswal, Biswal Mohan Ravindran, Ankathil RB Pathology RC Internal medicine The inhibitory protein IκBα, encoded by the NFKBIA gene, plays an important role in regulating the activity of nuclear factor-kappa B, a transcription factor which has been implicated in the initiation and progression of cancers. This study aimed to evaluate the association of NFKBIA −826C>T (rs2233406) and −881A>G (rs3138053) polymorphisms with the risk of sporadic colorectal cancer (CRC) in Malaysian population. A case–control study comprising 474 subjects (237 CRC patients and 237 cancer-free controls) was carried out. The polymorphisms were genotyped from the genomic DNA of the study subjects employing PCR–RFLP, followed by DNA sequencing. The association between the polymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95 % confidence intervals (CIs) using unconditional logistic regression analysis. The two polymorphisms were in complete and perfect linkage disequilibrium (D′ = 1.0, r 2 = 1.0). Overall, no statistically significant CRC risk association was found for the polymorphisms (P > 0.05). A similar lack of association was observed when the data were stratified according to ethnicity (P > 0.05). However, stratification by gender revealed a significant inverse association between the heterozygous genotype of the polymorphisms and the risk of CRC among females (OR 0.53, 95 % CI 0.29–0.97, P = 0.04), but not among males (P > 0.05). In conclusion, the heterozygous genotype of the polymorphisms could contribute to a significantly decreased CRC risk among females, but not males, in the Malaysian population. Springer US 2013 Article PeerReviewed text en http://ir.unimas.my/id/eprint/11980/1/Gender-specific%20association%20of%20NFKBIA%20promoter%20polymorphisms%20%28abstract%29.pdf Tan, Shing Cheng and Mohd Shafi’i, Mohd Suzairi and Abdul Aziz, Ahmad Aizat and Mustapha, Mohd Aminudin and Siti Nurfatimah, Mohd Shahpudin and Venkata Murali, Krishna Bhavaraju and Biswal, Biswal Mohan and Ravindran, Ankathil (2013) Gender-specific association of NFKBIA promoter polymorphisms with the risk of sporadic colorectal cancer. Medical Oncology, 30 (693). ISSN 1559-131X http://link.springer.com/article/10.1007%2Fs12032-013-0693-6 DOI : 10.1007/s12032-013-0693-6
spellingShingle RB Pathology
RC Internal medicine
Tan, Shing Cheng
Mohd Shafi’i, Mohd Suzairi
Abdul Aziz, Ahmad Aizat
Mustapha, Mohd Aminudin
Siti Nurfatimah, Mohd Shahpudin
Venkata Murali, Krishna Bhavaraju
Biswal, Biswal Mohan
Ravindran, Ankathil
Gender-specific association of NFKBIA promoter polymorphisms with the risk of sporadic colorectal cancer
title Gender-specific association of NFKBIA promoter polymorphisms with the risk of sporadic colorectal cancer
title_full Gender-specific association of NFKBIA promoter polymorphisms with the risk of sporadic colorectal cancer
title_fullStr Gender-specific association of NFKBIA promoter polymorphisms with the risk of sporadic colorectal cancer
title_full_unstemmed Gender-specific association of NFKBIA promoter polymorphisms with the risk of sporadic colorectal cancer
title_short Gender-specific association of NFKBIA promoter polymorphisms with the risk of sporadic colorectal cancer
title_sort gender-specific association of nfkbia promoter polymorphisms with the risk of sporadic colorectal cancer
topic RB Pathology
RC Internal medicine
url http://ir.unimas.my/id/eprint/11980/
http://ir.unimas.my/id/eprint/11980/
http://ir.unimas.my/id/eprint/11980/
http://ir.unimas.my/id/eprint/11980/1/Gender-specific%20association%20of%20NFKBIA%20promoter%20polymorphisms%20%28abstract%29.pdf