Pathological findings in a mouse model for Coxsackievirus A16 infection

Coxsackievirus A16 (CV-A16) is the leading cause of hand-foot-mouth disease (HFMD), which usually presents as mild and self-limiting symptoms in young children. Rarely, CV-A16 has been reported to cause severe and fatal neurological complications but little is known about these complications. In...

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Main Authors: Yuan, Teng Hooi, Kien, Chai Ong, Perera, David, Kum, Thong Wong
Format: Article
Language:English
Published: ASEAN Neurological Association (ASNA), Asian & Oceanian Association of Neurology (AOAN), and the Asian & Oceanian Child Neurology Association 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/11959/
http://ir.unimas.my/id/eprint/11959/1/No%206%20%28abstrak%29.pdf
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author Yuan, Teng Hooi
Kien, Chai Ong
Perera, David
Kum, Thong Wong
author_facet Yuan, Teng Hooi
Kien, Chai Ong
Perera, David
Kum, Thong Wong
author_sort Yuan, Teng Hooi
building UNIMAS Institutional Repository
collection Online Access
description Coxsackievirus A16 (CV-A16) is the leading cause of hand-foot-mouth disease (HFMD), which usually presents as mild and self-limiting symptoms in young children. Rarely, CV-A16 has been reported to cause severe and fatal neurological complications but little is known about these complications. In the present study, 1-day and 7-day old mouse models of CV-A16 were developed using a clinical strain via subcutaneous inoculation. All infected mice exhibited clinical signs of infection, including reduced mobility, limb weakness and paralysis between 3 to 6 days post-infection. Pathologically, the main organs involved were the central nervous system (CNS), skeletal muscles and brown fat. In the CNS, viral antigens as demonstrated by immunohistochemistry, were localized mainly to neurons in the brain stem and spinal cord, suggesting that CV-A16 is neurotropic although inflammation is very mild. The skeletal muscles showed necrosis and myositis due to viral infection as evidenced by the dense viral antigens. Focal viral antigens were also detected in the brown fat. These preliminary pathological findings indicate that our mouse models can be further developed to be useful models for pathogenesis studies, and vaccine and anti-viral drug evaluation.
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spelling unimas-119592016-10-24T07:17:26Z http://ir.unimas.my/id/eprint/11959/ Pathological findings in a mouse model for Coxsackievirus A16 infection Yuan, Teng Hooi Kien, Chai Ong Perera, David Kum, Thong Wong RM Therapeutics. Pharmacology Coxsackievirus A16 (CV-A16) is the leading cause of hand-foot-mouth disease (HFMD), which usually presents as mild and self-limiting symptoms in young children. Rarely, CV-A16 has been reported to cause severe and fatal neurological complications but little is known about these complications. In the present study, 1-day and 7-day old mouse models of CV-A16 were developed using a clinical strain via subcutaneous inoculation. All infected mice exhibited clinical signs of infection, including reduced mobility, limb weakness and paralysis between 3 to 6 days post-infection. Pathologically, the main organs involved were the central nervous system (CNS), skeletal muscles and brown fat. In the CNS, viral antigens as demonstrated by immunohistochemistry, were localized mainly to neurons in the brain stem and spinal cord, suggesting that CV-A16 is neurotropic although inflammation is very mild. The skeletal muscles showed necrosis and myositis due to viral infection as evidenced by the dense viral antigens. Focal viral antigens were also detected in the brown fat. These preliminary pathological findings indicate that our mouse models can be further developed to be useful models for pathogenesis studies, and vaccine and anti-viral drug evaluation. ASEAN Neurological Association (ASNA), Asian & Oceanian Association of Neurology (AOAN), and the Asian & Oceanian Child Neurology Association 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/11959/1/No%206%20%28abstrak%29.pdf Yuan, Teng Hooi and Kien, Chai Ong and Perera, David and Kum, Thong Wong (2015) Pathological findings in a mouse model for Coxsackievirus A16 infection. Neurology Asia, 20 (3). pp. 343-347. ISSN 1823-6138 http://www.neurology-asia.org/journal.php
spellingShingle RM Therapeutics. Pharmacology
Yuan, Teng Hooi
Kien, Chai Ong
Perera, David
Kum, Thong Wong
Pathological findings in a mouse model for Coxsackievirus A16 infection
title Pathological findings in a mouse model for Coxsackievirus A16 infection
title_full Pathological findings in a mouse model for Coxsackievirus A16 infection
title_fullStr Pathological findings in a mouse model for Coxsackievirus A16 infection
title_full_unstemmed Pathological findings in a mouse model for Coxsackievirus A16 infection
title_short Pathological findings in a mouse model for Coxsackievirus A16 infection
title_sort pathological findings in a mouse model for coxsackievirus a16 infection
topic RM Therapeutics. Pharmacology
url http://ir.unimas.my/id/eprint/11959/
http://ir.unimas.my/id/eprint/11959/
http://ir.unimas.my/id/eprint/11959/1/No%206%20%28abstrak%29.pdf