Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment
Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to the family Pseudomonadaceae. The species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment,...
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| Format: | Article |
| Language: | English |
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Pelagia Research
2013
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| Online Access: | http://ir.unimas.my/id/eprint/1170/ http://ir.unimas.my/id/eprint/1170/1/Detection.pdf |
| _version_ | 1848834704084566016 |
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| author | Rakiya, Abdullahi Samuel, Lihan B. S., Carlos Lesley, Maurice Bilung M. K., Mikal Felicia, Collick |
| author_facet | Rakiya, Abdullahi Samuel, Lihan B. S., Carlos Lesley, Maurice Bilung M. K., Mikal Felicia, Collick |
| author_sort | Rakiya, Abdullahi |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to the family Pseudomonadaceae. The
species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including
aquaculture environment. Antibiotics are used in the aquaculture environment, and their improper usage poses a
risk of potential transfer of resistance from aquaculture bacteria to human and animal pathogens. This study was
conducted to isolate P. aeruginosa from fish, prawn and water samples, followed by PCR detection of oprL gene
locus. The antibiotic resistance pattern of the isolates was also determined. Based on the results from PCR analysis
performed, 13 isolates of P. aeruginosawere isolated. All of the isolates tested were resistance to at least one
antibiotic. Highest level of resistance was observed against ampicillin and erythromycin while the lowest was
observed against gentamicin, norfloxacin and nalidixic acid. This study suggested that the presence of the bacteria
in the aquaculture environment may pose the risk of antibiotic resistance to those who are exposed to the
aquaculture environment.Based on the results of this study, it can be said that gentamicin, norflaxin and nalidixic
acid can be effective agents for the treatment of P. aeruginosa. |
| first_indexed | 2025-11-15T05:56:12Z |
| format | Article |
| id | unimas-1170 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T05:56:12Z |
| publishDate | 2013 |
| publisher | Pelagia Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-11702022-01-25T07:58:49Z http://ir.unimas.my/id/eprint/1170/ Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment Rakiya, Abdullahi Samuel, Lihan B. S., Carlos Lesley, Maurice Bilung M. K., Mikal Felicia, Collick S Agriculture (General) SH Aquaculture. Fisheries. Angling Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to the family Pseudomonadaceae. The species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment, and their improper usage poses a risk of potential transfer of resistance from aquaculture bacteria to human and animal pathogens. This study was conducted to isolate P. aeruginosa from fish, prawn and water samples, followed by PCR detection of oprL gene locus. The antibiotic resistance pattern of the isolates was also determined. Based on the results from PCR analysis performed, 13 isolates of P. aeruginosawere isolated. All of the isolates tested were resistance to at least one antibiotic. Highest level of resistance was observed against ampicillin and erythromycin while the lowest was observed against gentamicin, norfloxacin and nalidixic acid. This study suggested that the presence of the bacteria in the aquaculture environment may pose the risk of antibiotic resistance to those who are exposed to the aquaculture environment.Based on the results of this study, it can be said that gentamicin, norflaxin and nalidixic acid can be effective agents for the treatment of P. aeruginosa. Pelagia Research 2013 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/1170/1/Detection.pdf Rakiya, Abdullahi and Samuel, Lihan and B. S., Carlos and Lesley, Maurice Bilung and M. K., Mikal and Felicia, Collick (2013) Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment. European Journal of Experimental Biology, 3 (6). pp. 148-152. ISSN 2248-9215 http://www.pelagiaresearchlibrary.com |
| spellingShingle | S Agriculture (General) SH Aquaculture. Fisheries. Angling Rakiya, Abdullahi Samuel, Lihan B. S., Carlos Lesley, Maurice Bilung M. K., Mikal Felicia, Collick Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment |
| title | Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment |
| title_full | Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment |
| title_fullStr | Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment |
| title_full_unstemmed | Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment |
| title_short | Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment |
| title_sort | detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture environment |
| topic | S Agriculture (General) SH Aquaculture. Fisheries. Angling |
| url | http://ir.unimas.my/id/eprint/1170/ http://ir.unimas.my/id/eprint/1170/ http://ir.unimas.my/id/eprint/1170/1/Detection.pdf |