On-site rapid detection of toxic Alexandrium tamiyavanichii: integrating the species-specific hydrolysis probe in insulated isothermal polymerase chain reaction (iiPCR)
On-site investigation of phytoplankton samples is important for rapid detection of harmful algal species and for early warning of harmful algal bloom. Molecular detection method by DNA amplification in a portable insulated isothermal PCR (iiPCR) device provides a simple and rapid detection base...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Springer Netherlands
2016
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| Online Access: | http://ir.unimas.my/id/eprint/11492/ http://ir.unimas.my/id/eprint/11492/1/On-site%20rapid%20detection%20of%20toxic%20Alexandrium%20tamiyavanichii%20%28abstract%29.pdf |
| _version_ | 1848837022025777152 |
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| author | Nyuk, Fong Kon Lik, Winne Sing Lau Ing, Kuo Law Po, Teen Lim Chui, Pin Leaw |
| author_facet | Nyuk, Fong Kon Lik, Winne Sing Lau Ing, Kuo Law Po, Teen Lim Chui, Pin Leaw |
| author_sort | Nyuk, Fong Kon |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | On-site investigation of phytoplankton samples is
important for rapid detection of harmful algal species and for
early warning of harmful algal bloom. Molecular detection
method by DNA amplification in a portable insulated isothermal
PCR (iiPCR) device provides a simple and rapid detection
based on fluorescent probe within an hour of reaction time.
The assay was developed for a paralytic shellfish toxinproducing
dinoflagellate Alexandrium tamiyavanichii. The assay
presents the data as positive or negative on the presence or
absence of A. tamiyavanichii cells, with a limit of detection
(LOD) at five target cells per reaction. While the assay is
incapable to accurately quantify cell density, it exhibits high
detection accuracy and strongly correlated with quantitative
PCR (qPCR) data. The user repeatability of iiPCR assay was
evaluated; the results showed that no significant differences in
the assay run by different operators. Field applicability of the
assay was further validated by environmental samples.
Despite the shortcoming of the assay, the overall performance
of the assay to detect cells, its low-cost effectiveness, and
portability for on-site detection, iiPCR has proven its potential
as an early screening tool for harmful algae monitoring.
Nyuk Fong Kon1 & Winnie Lik Sing Lau2 & Ing Kuo Law2 & Po Teen Lim2 &
Chui Pin Leaw2 |
| first_indexed | 2025-11-15T06:33:03Z |
| format | Article |
| id | unimas-11492 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:33:03Z |
| publishDate | 2016 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-114922016-10-21T01:51:08Z http://ir.unimas.my/id/eprint/11492/ On-site rapid detection of toxic Alexandrium tamiyavanichii: integrating the species-specific hydrolysis probe in insulated isothermal polymerase chain reaction (iiPCR) Nyuk, Fong Kon Lik, Winne Sing Lau Ing, Kuo Law Po, Teen Lim Chui, Pin Leaw GE Environmental Sciences On-site investigation of phytoplankton samples is important for rapid detection of harmful algal species and for early warning of harmful algal bloom. Molecular detection method by DNA amplification in a portable insulated isothermal PCR (iiPCR) device provides a simple and rapid detection based on fluorescent probe within an hour of reaction time. The assay was developed for a paralytic shellfish toxinproducing dinoflagellate Alexandrium tamiyavanichii. The assay presents the data as positive or negative on the presence or absence of A. tamiyavanichii cells, with a limit of detection (LOD) at five target cells per reaction. While the assay is incapable to accurately quantify cell density, it exhibits high detection accuracy and strongly correlated with quantitative PCR (qPCR) data. The user repeatability of iiPCR assay was evaluated; the results showed that no significant differences in the assay run by different operators. Field applicability of the assay was further validated by environmental samples. Despite the shortcoming of the assay, the overall performance of the assay to detect cells, its low-cost effectiveness, and portability for on-site detection, iiPCR has proven its potential as an early screening tool for harmful algae monitoring. Nyuk Fong Kon1 & Winnie Lik Sing Lau2 & Ing Kuo Law2 & Po Teen Lim2 & Chui Pin Leaw2 Springer Netherlands 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/11492/1/On-site%20rapid%20detection%20of%20toxic%20Alexandrium%20tamiyavanichii%20%28abstract%29.pdf Nyuk, Fong Kon and Lik, Winne Sing Lau and Ing, Kuo Law and Po, Teen Lim and Chui, Pin Leaw (2016) On-site rapid detection of toxic Alexandrium tamiyavanichii: integrating the species-specific hydrolysis probe in insulated isothermal polymerase chain reaction (iiPCR). Journal of Applied Phycology, 2016. pp. 1-6. ISSN 1573-5176 https://www.scopus.com/inward/record.url?eid=2-s2.0-84955591536&partnerID=40&md5=19c39e40a8da2d8a7a7da9d9a58d4e65 DOI 10.1007/s10811-016-0802-y |
| spellingShingle | GE Environmental Sciences Nyuk, Fong Kon Lik, Winne Sing Lau Ing, Kuo Law Po, Teen Lim Chui, Pin Leaw On-site rapid detection of toxic Alexandrium tamiyavanichii: integrating the species-specific hydrolysis probe in insulated isothermal polymerase chain reaction (iiPCR) |
| title | On-site rapid detection of toxic Alexandrium tamiyavanichii:
integrating the species-specific hydrolysis probe in insulated
isothermal polymerase chain reaction (iiPCR) |
| title_full | On-site rapid detection of toxic Alexandrium tamiyavanichii:
integrating the species-specific hydrolysis probe in insulated
isothermal polymerase chain reaction (iiPCR) |
| title_fullStr | On-site rapid detection of toxic Alexandrium tamiyavanichii:
integrating the species-specific hydrolysis probe in insulated
isothermal polymerase chain reaction (iiPCR) |
| title_full_unstemmed | On-site rapid detection of toxic Alexandrium tamiyavanichii:
integrating the species-specific hydrolysis probe in insulated
isothermal polymerase chain reaction (iiPCR) |
| title_short | On-site rapid detection of toxic Alexandrium tamiyavanichii:
integrating the species-specific hydrolysis probe in insulated
isothermal polymerase chain reaction (iiPCR) |
| title_sort | on-site rapid detection of toxic alexandrium tamiyavanichii:
integrating the species-specific hydrolysis probe in insulated
isothermal polymerase chain reaction (iipcr) |
| topic | GE Environmental Sciences |
| url | http://ir.unimas.my/id/eprint/11492/ http://ir.unimas.my/id/eprint/11492/ http://ir.unimas.my/id/eprint/11492/ http://ir.unimas.my/id/eprint/11492/1/On-site%20rapid%20detection%20of%20toxic%20Alexandrium%20tamiyavanichii%20%28abstract%29.pdf |