An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)2(qtpy)]Cl2
Non toxicity and high isoelectric point of zinc oxide nanorods (ZnO NRs) are the promising materials widely used in biomolecule detection and biomedicine application. An electrochemical DNA biosensor was successfully fabricated by depositing (3-Aminopropyl)triethoxysilane and gold nanoparticles (GNP...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer Berlin Heidelberg
2016
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| Online Access: | http://ir.unimas.my/id/eprint/11467/ http://ir.unimas.my/id/eprint/11467/1/An%20electrochemical%20DNA%20biosensor%20based%20gold%E2%80%91thiolate%20%28abstract%29.pdf |
| _version_ | 1848837016473567232 |
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| author | Foo, K. L. M., Kashif Tan, S. J. U., Hashim |
| author_facet | Foo, K. L. M., Kashif Tan, S. J. U., Hashim |
| author_sort | Foo, K. L. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Non toxicity and high isoelectric point of zinc oxide nanorods (ZnO NRs) are the promising materials widely used in biomolecule detection and biomedicine application. An electrochemical DNA biosensor was successfully fabricated by depositing (3-Aminopropyl)triethoxysilane and gold nanoparticles (GNPs) on the hydrothermal growth ZnO NRs. The modified surface with GNPs (35 ± 5 nm) was characterized by field emission scanning electron microscopy with energy dispersive X-ray and X-ray diffraction. The fabricated electrode was used for the label-free detection of DNA. Thiolated (–SH) single stranded DNA was immobilized onto the GNPs surface to form a gold-thiolate bond. Hybridization with complementary target DNA was performed with the presence of ruthenium complex. Immobilization and hybridization detection were performed using µAUTOLAB with cyclic voltammetric software. The measured sensitivity of the fabricated electrode for hybridization is up to 1.6 times larger than immobilization. |
| first_indexed | 2025-11-15T06:32:57Z |
| format | Article |
| id | unimas-11467 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:32:57Z |
| publishDate | 2016 |
| publisher | Springer Berlin Heidelberg |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-114672016-10-21T01:43:48Z http://ir.unimas.my/id/eprint/11467/ An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)2(qtpy)]Cl2 Foo, K. L. M., Kashif Tan, S. J. U., Hashim T Technology (General) TK Electrical engineering. Electronics Nuclear engineering Non toxicity and high isoelectric point of zinc oxide nanorods (ZnO NRs) are the promising materials widely used in biomolecule detection and biomedicine application. An electrochemical DNA biosensor was successfully fabricated by depositing (3-Aminopropyl)triethoxysilane and gold nanoparticles (GNPs) on the hydrothermal growth ZnO NRs. The modified surface with GNPs (35 ± 5 nm) was characterized by field emission scanning electron microscopy with energy dispersive X-ray and X-ray diffraction. The fabricated electrode was used for the label-free detection of DNA. Thiolated (–SH) single stranded DNA was immobilized onto the GNPs surface to form a gold-thiolate bond. Hybridization with complementary target DNA was performed with the presence of ruthenium complex. Immobilization and hybridization detection were performed using µAUTOLAB with cyclic voltammetric software. The measured sensitivity of the fabricated electrode for hybridization is up to 1.6 times larger than immobilization. Springer Berlin Heidelberg 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/11467/1/An%20electrochemical%20DNA%20biosensor%20based%20gold%E2%80%91thiolate%20%28abstract%29.pdf Foo, K. L. and M., Kashif and Tan, S. J. and U., Hashim (2016) An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)2(qtpy)]Cl2. Microsystem Technologies, 2016. pp. 1-9. ISSN 1432-1858 http://link.springer.com/article/10.1007%2Fs00542-016-2874-7 DOI 10.1007/s00542-016-2874-7 |
| spellingShingle | T Technology (General) TK Electrical engineering. Electronics Nuclear engineering Foo, K. L. M., Kashif Tan, S. J. U., Hashim An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)2(qtpy)]Cl2 |
| title | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)2(qtpy)]Cl2 |
| title_full | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)2(qtpy)]Cl2 |
| title_fullStr | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)2(qtpy)]Cl2 |
| title_full_unstemmed | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)2(qtpy)]Cl2 |
| title_short | An electrochemical DNA biosensor based gold-thiolate conjugation utilizing ruthenium complex [Ru(dppz)2(qtpy)]Cl2 |
| title_sort | electrochemical dna biosensor based gold-thiolate conjugation utilizing ruthenium complex [ru(dppz)2(qtpy)]cl2 |
| topic | T Technology (General) TK Electrical engineering. Electronics Nuclear engineering |
| url | http://ir.unimas.my/id/eprint/11467/ http://ir.unimas.my/id/eprint/11467/ http://ir.unimas.my/id/eprint/11467/ http://ir.unimas.my/id/eprint/11467/1/An%20electrochemical%20DNA%20biosensor%20based%20gold%E2%80%91thiolate%20%28abstract%29.pdf |