Partial identification of genomic HPPR gene in Orthosiphon aristatus
Orthosiphon aristatus (Lamiaceae), locally known as Misai Kucing is a well known medicinal plant which has been used as traditional medicine to treat various human ailments. Many bioactive compound have been identified in medicinal plants which make it beneficial to human health. O. aristatus exhibi...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/11039/ http://ir.unimas.my/id/eprint/11039/1/Ellya%20Aqeela%20ft.pdf |
| _version_ | 1848836919755014144 |
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| author | Mohd Fauzi., Ellya Aqeela |
| author_facet | Mohd Fauzi., Ellya Aqeela |
| author_sort | Mohd Fauzi., Ellya Aqeela |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Orthosiphon aristatus (Lamiaceae), locally known as Misai Kucing is a well known medicinal plant which has been used as traditional medicine to treat various human ailments. Many bioactive compound have been identified in medicinal plants which make it beneficial to human health. O. aristatus exhibits high content of secondary metabolites such as rosmarinic acid, a caffeic acid derivative which causes diuretic and therapeutic effects. Hydroxyphenylpyruvate reductase (HPPR) is one of the enzymes that involve in the rosmarinic acid biosynthesis. The focus of this study is to isolate and characterize a genomic HPPR gene from O. aristatus. The isolated HPPR gene was successfully amplified via polymerase chain reaction (PCR). An approximately 2000 bp nucleotide genomic DNA was isolated and further analysed for homology by Blastn. The homology analysis revealed high sequence identity to other plants which contain HPPR gene. |
| first_indexed | 2025-11-15T06:31:25Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-11039 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:31:25Z |
| publishDate | 2015 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-110392023-12-21T01:46:19Z http://ir.unimas.my/id/eprint/11039/ Partial identification of genomic HPPR gene in Orthosiphon aristatus Mohd Fauzi., Ellya Aqeela RM Therapeutics. Pharmacology Orthosiphon aristatus (Lamiaceae), locally known as Misai Kucing is a well known medicinal plant which has been used as traditional medicine to treat various human ailments. Many bioactive compound have been identified in medicinal plants which make it beneficial to human health. O. aristatus exhibits high content of secondary metabolites such as rosmarinic acid, a caffeic acid derivative which causes diuretic and therapeutic effects. Hydroxyphenylpyruvate reductase (HPPR) is one of the enzymes that involve in the rosmarinic acid biosynthesis. The focus of this study is to isolate and characterize a genomic HPPR gene from O. aristatus. The isolated HPPR gene was successfully amplified via polymerase chain reaction (PCR). An approximately 2000 bp nucleotide genomic DNA was isolated and further analysed for homology by Blastn. The homology analysis revealed high sequence identity to other plants which contain HPPR gene. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/11039/1/Ellya%20Aqeela%20ft.pdf Mohd Fauzi., Ellya Aqeela (2015) Partial identification of genomic HPPR gene in Orthosiphon aristatus. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | RM Therapeutics. Pharmacology Mohd Fauzi., Ellya Aqeela Partial identification of genomic HPPR gene in Orthosiphon aristatus |
| title | Partial identification of genomic HPPR gene in Orthosiphon aristatus |
| title_full | Partial identification of genomic HPPR gene in Orthosiphon aristatus |
| title_fullStr | Partial identification of genomic HPPR gene in Orthosiphon aristatus |
| title_full_unstemmed | Partial identification of genomic HPPR gene in Orthosiphon aristatus |
| title_short | Partial identification of genomic HPPR gene in Orthosiphon aristatus |
| title_sort | partial identification of genomic hppr gene in orthosiphon aristatus |
| topic | RM Therapeutics. Pharmacology |
| url | http://ir.unimas.my/id/eprint/11039/ http://ir.unimas.my/id/eprint/11039/1/Ellya%20Aqeela%20ft.pdf |