Nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media

Nitrogen source is very important in the production of the bioethanol. Starchy materials such as sago 'hampas' which can be found abundantly in Malaysia is one of the suitable sources for the production of bioethanol. The main focus of this study is to find out which type of nitrogen sourc...

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Main Author: Jomoi, Alfrida
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/11038/
http://ir.unimas.my/id/eprint/11038/9/Nitrogen%20Supplementation%20With%20Sago%20%E2%80%98Hampas%E2%80%99%20Hydrolysate%20For%20Low%20Cost%20Bioethanol%20Fermentation%20Media.pdf
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author Jomoi, Alfrida
author_facet Jomoi, Alfrida
author_sort Jomoi, Alfrida
building UNIMAS Institutional Repository
collection Online Access
description Nitrogen source is very important in the production of the bioethanol. Starchy materials such as sago 'hampas' which can be found abundantly in Malaysia is one of the suitable sources for the production of bioethanol. The main focus of this study is to find out which type of nitrogen sources is the best for the fennentation of bioethanol. Previous studies showed that the best nitrogen source is the yeast extract. However, yeast extracts are expensive and this research also focus on to find which nitrogen sources are cheaper to replace yeast extract and also may decrease the production cost. Four types of nitrogen sources were used namely peptone, ammonium sulphate, yeast extract and urea. The effect of these different sources of nitrogen in the production ofbioethanol were evaluated in this study. Sago 'hampas' was treated with the Liquozyme and Spirizyme for the saccharification of starch and undergo three cycles of enzymatic hydrolysis in order to get sago 'hampas' hydrolysate (SHH). Two replicates were prepared for each type of nitrogen sources. The SHH were fermented by yeast or Saccharomyces cerevisiae in 250 mL of Erlenmeyer flask with 100 mL of media and batch fermentation will be used in this study. From this study SHH were able to provide glucose needed during fermentation in the production of bioethanol. Urea, peptone and ammonium sulfate can replaced the yeast extract as the sources ofnitrogen in fermentation.
first_indexed 2025-11-15T06:31:25Z
format Final Year Project Report / IMRAD
id unimas-11038
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:31:25Z
publishDate 2015
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-110382023-06-28T07:01:25Z http://ir.unimas.my/id/eprint/11038/ Nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media Jomoi, Alfrida QD Chemistry S Agriculture (General) Nitrogen source is very important in the production of the bioethanol. Starchy materials such as sago 'hampas' which can be found abundantly in Malaysia is one of the suitable sources for the production of bioethanol. The main focus of this study is to find out which type of nitrogen sources is the best for the fennentation of bioethanol. Previous studies showed that the best nitrogen source is the yeast extract. However, yeast extracts are expensive and this research also focus on to find which nitrogen sources are cheaper to replace yeast extract and also may decrease the production cost. Four types of nitrogen sources were used namely peptone, ammonium sulphate, yeast extract and urea. The effect of these different sources of nitrogen in the production ofbioethanol were evaluated in this study. Sago 'hampas' was treated with the Liquozyme and Spirizyme for the saccharification of starch and undergo three cycles of enzymatic hydrolysis in order to get sago 'hampas' hydrolysate (SHH). Two replicates were prepared for each type of nitrogen sources. The SHH were fermented by yeast or Saccharomyces cerevisiae in 250 mL of Erlenmeyer flask with 100 mL of media and batch fermentation will be used in this study. From this study SHH were able to provide glucose needed during fermentation in the production of bioethanol. Urea, peptone and ammonium sulfate can replaced the yeast extract as the sources ofnitrogen in fermentation. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/11038/9/Nitrogen%20Supplementation%20With%20Sago%20%E2%80%98Hampas%E2%80%99%20Hydrolysate%20For%20Low%20Cost%20Bioethanol%20Fermentation%20Media.pdf Jomoi, Alfrida (2015) Nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QD Chemistry
S Agriculture (General)
Jomoi, Alfrida
Nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media
title Nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media
title_full Nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media
title_fullStr Nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media
title_full_unstemmed Nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media
title_short Nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media
title_sort nitrogen supplementation with sago 'hampas' hydrolysate for low cost bioethanol fermentation media
topic QD Chemistry
S Agriculture (General)
url http://ir.unimas.my/id/eprint/11038/
http://ir.unimas.my/id/eprint/11038/9/Nitrogen%20Supplementation%20With%20Sago%20%E2%80%98Hampas%E2%80%99%20Hydrolysate%20For%20Low%20Cost%20Bioethanol%20Fermentation%20Media.pdf