Isolation and cloning of ABCA2 gene from rasbora sarawakensis

ABC genes encode ABC transporter protein that plays an important role in ATP hydrolysis in which to assist the solute to cross the plasma membrane. Mutation of ABC genes lead to human diseases such as Alzheimer, PFIC, and PXE. The aim of this research is to identify the expression of ABCA2 gene in R...

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Main Author: Tan, Say Yen.
Format: Final Year Project Report / IMRAD
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/11027/
http://ir.unimas.my/id/eprint/11027/1/Say%20Yen%20%2824%20pages%29.pdf
http://ir.unimas.my/id/eprint/11027/4/Tan%20Say%20Yen.pdf
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author Tan, Say Yen.
author_facet Tan, Say Yen.
author_sort Tan, Say Yen.
building UNIMAS Institutional Repository
collection Online Access
description ABC genes encode ABC transporter protein that plays an important role in ATP hydrolysis in which to assist the solute to cross the plasma membrane. Mutation of ABC genes lead to human diseases such as Alzheimer, PFIC, and PXE. The aim of this research is to identify the expression of ABCA2 gene in Rasbora sarawakensis and subsequently clone into pGEM-T® Easy vector. Total RNA was initially isolated from whole fish homogenate using Tri reagent and phenol chloroform precipitation.
first_indexed 2025-11-15T06:31:23Z
format Final Year Project Report / IMRAD
id unimas-11027
institution Universiti Malaysia Sarawak
institution_category Local University
language English
English
last_indexed 2025-11-15T06:31:23Z
publishDate 2015
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-110272024-04-01T07:43:56Z http://ir.unimas.my/id/eprint/11027/ Isolation and cloning of ABCA2 gene from rasbora sarawakensis Tan, Say Yen. RS Pharmacy and materia medica SH Aquaculture. Fisheries. Angling ABC genes encode ABC transporter protein that plays an important role in ATP hydrolysis in which to assist the solute to cross the plasma membrane. Mutation of ABC genes lead to human diseases such as Alzheimer, PFIC, and PXE. The aim of this research is to identify the expression of ABCA2 gene in Rasbora sarawakensis and subsequently clone into pGEM-T® Easy vector. Total RNA was initially isolated from whole fish homogenate using Tri reagent and phenol chloroform precipitation. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/11027/1/Say%20Yen%20%2824%20pages%29.pdf text en http://ir.unimas.my/id/eprint/11027/4/Tan%20Say%20Yen.pdf Tan, Say Yen. (2015) Isolation and cloning of ABCA2 gene from rasbora sarawakensis. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle RS Pharmacy and materia medica
SH Aquaculture. Fisheries. Angling
Tan, Say Yen.
Isolation and cloning of ABCA2 gene from rasbora sarawakensis
title Isolation and cloning of ABCA2 gene from rasbora sarawakensis
title_full Isolation and cloning of ABCA2 gene from rasbora sarawakensis
title_fullStr Isolation and cloning of ABCA2 gene from rasbora sarawakensis
title_full_unstemmed Isolation and cloning of ABCA2 gene from rasbora sarawakensis
title_short Isolation and cloning of ABCA2 gene from rasbora sarawakensis
title_sort isolation and cloning of abca2 gene from rasbora sarawakensis
topic RS Pharmacy and materia medica
SH Aquaculture. Fisheries. Angling
url http://ir.unimas.my/id/eprint/11027/
http://ir.unimas.my/id/eprint/11027/1/Say%20Yen%20%2824%20pages%29.pdf
http://ir.unimas.my/id/eprint/11027/4/Tan%20Say%20Yen.pdf