A Molecular Phylogeny Of Bornean Tree Frog (Amphibia : Anura : Rhacophoridae) From Genus Polypedates
The evolutionary biology of genus Polypedates was not well understood. Thus, molecular phylogeny of the genus Polypedates were studied by using mitochondrial ribosomal of partial 16S gene, in order to elucidate the phylogenetic relationship among members of genus. Tissues samples were collected f...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2011
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| Online Access: | http://ir.unimas.my/id/eprint/1100/ http://ir.unimas.my/id/eprint/1100/8/Sarina%20Mat%20Yasin.pdf |
| Summary: | The evolutionary biology of genus Polypedates was not well understood. Thus, molecular
phylogeny of the genus Polypedates were studied by using mitochondrial ribosomal of
partial 16S gene, in order to elucidate the phylogenetic relationship among members of
genus. Tissues samples were collected from six different localities, namely UNIMAS East
Campus, Bario, Similajau National Park, Bako National Park, Matang Wildlife Centre and
Kubah National Park. A total of 489 bp of mitochondrial ribosomal of partial 16S gene
from 25 samples were analysed using molecular technique approach (DNA extraction,
PCR amplification, and direct sequencing). From this study, there are two major
monophyletic groups were appeared and sister to each other supported by highly bootstrap
values of 100% in Neighbour-Joining (NJ), Maximum Parsimony (MP), Maximum
Likelihood (ML) and Bayesian Analysis. Hence, Polypedates otilophus was identified as
basal by outgroup rooting which fully supported by highly bootstrap values of NJ, MP, ML
and Bayesian Analysis. Moreover, Polypedates leucomystax, Polypedates macrotis,
Polypedates colletti and Polypedates otilophus were was sister groups. Mitochondrial
ribosomal 16S gene is a useful genetic marker for species divergence for all tree frogs and
this study should be extends to elucidate the phylogenetic analysis all species in the genus
including new species of Polypedates chlorophthalmus. |
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