Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities
Lipids extracted from the fruits of Litsea graciae and Litsea cubeba were selected for this study. The samples were extracted using Soxhlet extraction technique and analysed by Gas Chromatography-Mass Spectrometry, and chemical analysis such as acid value and saponification value. The average % (w/w...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Online Access: | http://ir.unimas.my/id/eprint/10999/ http://ir.unimas.my/id/eprint/10999/4/Jennyfull.pdf |
| _version_ | 1848836910856798208 |
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| author | Jenny Sze Woon, Pui |
| author_facet | Jenny Sze Woon, Pui |
| author_sort | Jenny Sze Woon, Pui |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Lipids extracted from the fruits of Litsea graciae and Litsea cubeba were selected for this study. The samples were extracted using Soxhlet extraction technique and analysed by Gas Chromatography-Mass Spectrometry, and chemical analysis such as acid value and saponification value. The average % (w/w) yield of lipid from L. graciae (2.18 % 2.06 % for fresh flesh; 3.12 % 0.31 % for fresh seed and 20.28 % 1.75 % for dried seed powder) and L. cubeba (6.17 % 1.17 %) was identified. Both lipids have an acid value of 29.05. L. cubeba gave saponification value of 108.46 3.24 while L. graciae gave 96.31 5.84 and 174.85 3.2 for the Soxhlet extracted flesh and dried seed powder, respectively. Palmitoleic acid was the most abundant unsaturated fatty acids present in the fresh flesh of L. graciae (26.55 %) and fresh seed of L. cubeba (20.35 %). For the saturated fatty acids composition of L. graciae, palmitic acid was mostly found in fresh flesh (15.20 %), lauric acid in both fresh seed (75.59 %) and dried seed powder (80.26 %). Stearic acid was the most composition in L. cubeba fresh seed (50.31 %). Biological activity such as brine shrimp lethality assay and antioxidant assay was carried out. Both L. graciae (LC50 = 639.04 ppm and 777.75 ppm for solvent soaking extraction and Soxhlet extraction respectively) and L. cubeba (LC50 = 779.71 ppm) oil showed to have a low toxicity as their LC50 (50 % mortality concentration) is over 500 ppm. EC50 (50 % effective concentration) values for L. graciae lipids (solvent soaking extracts = 3.09 μg/mL and Soxhlet extracts = 2.01 μg/mL), and Soxhlet extracted L. cubeba lipid (4.91 μg/mL) calculated were lower than that of ascorbic acid standard (11.12 3.47 μg/mL). |
| first_indexed | 2025-11-15T06:31:17Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-10999 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:31:17Z |
| publishDate | 2015 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-109992023-06-27T08:18:21Z http://ir.unimas.my/id/eprint/10999/ Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities Jenny Sze Woon, Pui QD Chemistry SB Plant culture Lipids extracted from the fruits of Litsea graciae and Litsea cubeba were selected for this study. The samples were extracted using Soxhlet extraction technique and analysed by Gas Chromatography-Mass Spectrometry, and chemical analysis such as acid value and saponification value. The average % (w/w) yield of lipid from L. graciae (2.18 % 2.06 % for fresh flesh; 3.12 % 0.31 % for fresh seed and 20.28 % 1.75 % for dried seed powder) and L. cubeba (6.17 % 1.17 %) was identified. Both lipids have an acid value of 29.05. L. cubeba gave saponification value of 108.46 3.24 while L. graciae gave 96.31 5.84 and 174.85 3.2 for the Soxhlet extracted flesh and dried seed powder, respectively. Palmitoleic acid was the most abundant unsaturated fatty acids present in the fresh flesh of L. graciae (26.55 %) and fresh seed of L. cubeba (20.35 %). For the saturated fatty acids composition of L. graciae, palmitic acid was mostly found in fresh flesh (15.20 %), lauric acid in both fresh seed (75.59 %) and dried seed powder (80.26 %). Stearic acid was the most composition in L. cubeba fresh seed (50.31 %). Biological activity such as brine shrimp lethality assay and antioxidant assay was carried out. Both L. graciae (LC50 = 639.04 ppm and 777.75 ppm for solvent soaking extraction and Soxhlet extraction respectively) and L. cubeba (LC50 = 779.71 ppm) oil showed to have a low toxicity as their LC50 (50 % mortality concentration) is over 500 ppm. EC50 (50 % effective concentration) values for L. graciae lipids (solvent soaking extracts = 3.09 μg/mL and Soxhlet extracts = 2.01 μg/mL), and Soxhlet extracted L. cubeba lipid (4.91 μg/mL) calculated were lower than that of ascorbic acid standard (11.12 3.47 μg/mL). Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10999/4/Jennyfull.pdf Jenny Sze Woon, Pui (2015) Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QD Chemistry SB Plant culture Jenny Sze Woon, Pui Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities |
| title | Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities |
| title_full | Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities |
| title_fullStr | Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities |
| title_full_unstemmed | Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities |
| title_short | Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities |
| title_sort | characterisation of lipids from litsea graciae and litsea cubeba fruits and their biological activities |
| topic | QD Chemistry SB Plant culture |
| url | http://ir.unimas.my/id/eprint/10999/ http://ir.unimas.my/id/eprint/10999/4/Jennyfull.pdf |