Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection
Cacao Swollen Shoot Disease (CSSD) is a persistent, incurable viral infection that kills infected cacao plants within 2-years after symptom development. Initially limited to West Africa, the disease has spread to other cacao growing areas, and poses a serious threat to the US$107 billion global choc...
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| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | http://ir.unimas.my/id/eprint/10926/ http://ir.unimas.my/id/eprint/10926/9/Joel%20Michael%20Ponniah%20ft.pdf |
| _version_ | 1848836892908322816 |
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| author | Joel Michael, Ponniah |
| author_facet | Joel Michael, Ponniah |
| author_sort | Joel Michael, Ponniah |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Cacao Swollen Shoot Disease (CSSD) is a persistent, incurable viral infection that kills infected cacao plants within 2-years after symptom development. Initially limited to West Africa, the disease has spread to other cacao growing areas, and poses a serious threat to the US$107 billion global chocolate industry. A prospective vaccine against CSSD was developed in this study using recombinant gene technology. Agou1 cacao swollen shoot virus genome was isolated from total plant DNA using an established PCRbased isolation methodology, and the resultant full-length linear virus DNA amplified in OneShot® Top10 chemically competent Escherichia coli cells using pCR-XL TOPO® cloning plasmids. The virus DNA was later retrieved, digested using EcoRI restriction enzyme, and the targeted ORF3 gene fragment isolated through gel electrophoresis and incorporated into pBAD-TOPO® expression plasmids. This were then transformed into domesticated Paenibacillus polymyxa cells through electroporation, which acted as vaccine carrier. The resultant Biotic Response Elicitor Vaccine (BREV) produced a fragment of the virus particle, which was hypothesized to induce augmented systemic expression of the natural plant defense mechanism. |
| first_indexed | 2025-11-15T06:30:59Z |
| format | Thesis |
| id | unimas-10926 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:30:59Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-109262025-06-16T02:35:28Z http://ir.unimas.my/id/eprint/10926/ Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection Joel Michael, Ponniah Q Science (General) SB Plant culture Cacao Swollen Shoot Disease (CSSD) is a persistent, incurable viral infection that kills infected cacao plants within 2-years after symptom development. Initially limited to West Africa, the disease has spread to other cacao growing areas, and poses a serious threat to the US$107 billion global chocolate industry. A prospective vaccine against CSSD was developed in this study using recombinant gene technology. Agou1 cacao swollen shoot virus genome was isolated from total plant DNA using an established PCRbased isolation methodology, and the resultant full-length linear virus DNA amplified in OneShot® Top10 chemically competent Escherichia coli cells using pCR-XL TOPO® cloning plasmids. The virus DNA was later retrieved, digested using EcoRI restriction enzyme, and the targeted ORF3 gene fragment isolated through gel electrophoresis and incorporated into pBAD-TOPO® expression plasmids. This were then transformed into domesticated Paenibacillus polymyxa cells through electroporation, which acted as vaccine carrier. The resultant Biotic Response Elicitor Vaccine (BREV) produced a fragment of the virus particle, which was hypothesized to induce augmented systemic expression of the natural plant defense mechanism. 2015 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/10926/9/Joel%20Michael%20Ponniah%20ft.pdf Joel Michael, Ponniah (2015) Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection. Masters thesis, Universiti Malaysia Sarawak, (UNIMAS). |
| spellingShingle | Q Science (General) SB Plant culture Joel Michael, Ponniah Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection |
| title | Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection |
| title_full | Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection |
| title_fullStr | Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection |
| title_full_unstemmed | Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection |
| title_short | Development of recombinant plant vaccine for cacao swollen shoot virus (CSSV) infection |
| title_sort | development of recombinant plant vaccine for cacao swollen shoot virus (cssv) infection |
| topic | Q Science (General) SB Plant culture |
| url | http://ir.unimas.my/id/eprint/10926/ http://ir.unimas.my/id/eprint/10926/9/Joel%20Michael%20Ponniah%20ft.pdf |