Documentation study on endophyte actinomycetes isolated from Senna Alata Linn in Kuching

Many studies have been done on Senna a/ala on its medicinal value such as treating skin diseases. Nevertheless, limited research was done on the endophytes that live in the Senna alata plants. The aim of this study is to isolate the endophyte actinomycetes from Senna a/ala plants; conduct extraction...

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Main Author: Ting, Jen Yi
Format: Thesis
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2011
Subjects:
Online Access:http://ir.unimas.my/id/eprint/10919/
http://ir.unimas.my/id/eprint/10919/2/Jen%20Yi%20%20%28fulltext%29.pdf
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author Ting, Jen Yi
author_facet Ting, Jen Yi
author_sort Ting, Jen Yi
building UNIMAS Institutional Repository
collection Online Access
description Many studies have been done on Senna a/ala on its medicinal value such as treating skin diseases. Nevertheless, limited research was done on the endophytes that live in the Senna alata plants. The aim of this study is to isolate the endophyte actinomycetes from Senna a/ala plants; conduct extraction process on the actinomycetes isolates and determine the potential of the isolates towards different types of pathogenic microorganisms. This study had used a combination of three methods in order to justify or classify the endophyte strains that had been isolated from Senna a/ala. These three methods are based on culture morphology, molecular classification approach and comparison to International Streptomyces Project (ISP) which acts as guideline to determine whether the articular endophyte actinomycetes are the unique strains or otherwise. In this study, a total of three putative indigenous endophyte actinomycetes have been isolated and they were subjected to fermentation and extraction process for antimicrobial ctivities test. In the antimicrobial test, five standard strains had been used. These five standard strains were the common pathogenic microorganisms that cause diseases. The results from antimicrobial test showed that all endophyte isolates demonstrate positive inhibition to the standard strains.
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institution Universiti Malaysia Sarawak
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language English
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spelling unimas-109192023-05-03T09:24:04Z http://ir.unimas.my/id/eprint/10919/ Documentation study on endophyte actinomycetes isolated from Senna Alata Linn in Kuching Ting, Jen Yi QR Microbiology SB Plant culture Many studies have been done on Senna a/ala on its medicinal value such as treating skin diseases. Nevertheless, limited research was done on the endophytes that live in the Senna alata plants. The aim of this study is to isolate the endophyte actinomycetes from Senna a/ala plants; conduct extraction process on the actinomycetes isolates and determine the potential of the isolates towards different types of pathogenic microorganisms. This study had used a combination of three methods in order to justify or classify the endophyte strains that had been isolated from Senna a/ala. These three methods are based on culture morphology, molecular classification approach and comparison to International Streptomyces Project (ISP) which acts as guideline to determine whether the articular endophyte actinomycetes are the unique strains or otherwise. In this study, a total of three putative indigenous endophyte actinomycetes have been isolated and they were subjected to fermentation and extraction process for antimicrobial ctivities test. In the antimicrobial test, five standard strains had been used. These five standard strains were the common pathogenic microorganisms that cause diseases. The results from antimicrobial test showed that all endophyte isolates demonstrate positive inhibition to the standard strains. Universiti Malaysia Sarawak, (UNIMAS) 2011 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/10919/2/Jen%20Yi%20%20%28fulltext%29.pdf Ting, Jen Yi (2011) Documentation study on endophyte actinomycetes isolated from Senna Alata Linn in Kuching. Masters thesis, Universiti Malaysia Sarawak, (UNIMAS).
spellingShingle QR Microbiology
SB Plant culture
Ting, Jen Yi
Documentation study on endophyte actinomycetes isolated from Senna Alata Linn in Kuching
title Documentation study on endophyte actinomycetes isolated from Senna Alata Linn in Kuching
title_full Documentation study on endophyte actinomycetes isolated from Senna Alata Linn in Kuching
title_fullStr Documentation study on endophyte actinomycetes isolated from Senna Alata Linn in Kuching
title_full_unstemmed Documentation study on endophyte actinomycetes isolated from Senna Alata Linn in Kuching
title_short Documentation study on endophyte actinomycetes isolated from Senna Alata Linn in Kuching
title_sort documentation study on endophyte actinomycetes isolated from senna alata linn in kuching
topic QR Microbiology
SB Plant culture
url http://ir.unimas.my/id/eprint/10919/
http://ir.unimas.my/id/eprint/10919/2/Jen%20Yi%20%20%28fulltext%29.pdf