Bioethanol production from sugarcane bagasse using escherichia coli K011
Sugarcane bagasse is one of the most abundant lignocellulosic biomass that can be found in Malaysia and this biomass can be used to produce bioethanol. In this study, bioethanol had been produced by using the Simultaneous co-Saccharification and Fermentation (Sc-SF) process. The addition of cellulas...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Online Access: | http://ir.unimas.my/id/eprint/10834/ http://ir.unimas.my/id/eprint/10834/13/Amira%20Dayana%20Binti%20Abd.%20Rahman%2024pgs.pdf http://ir.unimas.my/id/eprint/10834/16/Amira%20Dayana%20Binti%20Abd.%20Rahman%20ft.pdf |
| _version_ | 1848836870362890240 |
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| author | Amira Dayana, Abd. Rahman |
| author_facet | Amira Dayana, Abd. Rahman |
| author_sort | Amira Dayana, Abd. Rahman |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Sugarcane bagasse is one of the most abundant lignocellulosic biomass that can be found in Malaysia and this biomass can be used to produce bioethanol. In this study, bioethanol had been produced by using the Simultaneous co-Saccharification and Fermentation (Sc-SF) process. The addition of cellulase enzyme for the saccharification process had been added at the earliest stage of the fennentation process. Cellulase enzyme helped in the hydrolysis of cellulose to simpler substances such as glucose that later being used for the fermentation process. Escherichia coli KO II were used in this study as the ethanolic fennenters. Samples from each ofthe fennentation vessels had been analysed by using High Perfonnance Liquid Chromatography (HPLC). The samples were also being analysed for reducing sugars and total carbohydrate concentrations by using Phenol-Sulphuric Acid test and 3,5-dinitrosalicyclic acid (DNS) colorimetric method. The highest amount of bioethanol and acetic acid produced in Simultaneous co-Saccharification and Fennentation (ScSF) had been recorded by 2.50% fresh feedstock loading with the amount of 3.87 giL and 3.71 giL respectively. The best feedstock loading for the production of bioethanol is 2.50% fresh feedstock loading. This study indicated that it is possible to produce bioetha_nol without any pre-treatment on sugarcane bagasse. |
| first_indexed | 2025-11-15T06:30:38Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-10834 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:30:38Z |
| publishDate | 2015 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-108342023-02-14T02:32:20Z http://ir.unimas.my/id/eprint/10834/ Bioethanol production from sugarcane bagasse using escherichia coli K011 Amira Dayana, Abd. Rahman SB Plant culture Sugarcane bagasse is one of the most abundant lignocellulosic biomass that can be found in Malaysia and this biomass can be used to produce bioethanol. In this study, bioethanol had been produced by using the Simultaneous co-Saccharification and Fermentation (Sc-SF) process. The addition of cellulase enzyme for the saccharification process had been added at the earliest stage of the fennentation process. Cellulase enzyme helped in the hydrolysis of cellulose to simpler substances such as glucose that later being used for the fermentation process. Escherichia coli KO II were used in this study as the ethanolic fennenters. Samples from each ofthe fennentation vessels had been analysed by using High Perfonnance Liquid Chromatography (HPLC). The samples were also being analysed for reducing sugars and total carbohydrate concentrations by using Phenol-Sulphuric Acid test and 3,5-dinitrosalicyclic acid (DNS) colorimetric method. The highest amount of bioethanol and acetic acid produced in Simultaneous co-Saccharification and Fennentation (ScSF) had been recorded by 2.50% fresh feedstock loading with the amount of 3.87 giL and 3.71 giL respectively. The best feedstock loading for the production of bioethanol is 2.50% fresh feedstock loading. This study indicated that it is possible to produce bioetha_nol without any pre-treatment on sugarcane bagasse. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10834/13/Amira%20Dayana%20Binti%20Abd.%20Rahman%2024pgs.pdf text en http://ir.unimas.my/id/eprint/10834/16/Amira%20Dayana%20Binti%20Abd.%20Rahman%20ft.pdf Amira Dayana, Abd. Rahman (2015) Bioethanol production from sugarcane bagasse using escherichia coli K011. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | SB Plant culture Amira Dayana, Abd. Rahman Bioethanol production from sugarcane bagasse using escherichia coli K011 |
| title | Bioethanol production from sugarcane bagasse using escherichia coli K011 |
| title_full | Bioethanol production from sugarcane bagasse using escherichia coli K011 |
| title_fullStr | Bioethanol production from sugarcane bagasse using escherichia coli K011 |
| title_full_unstemmed | Bioethanol production from sugarcane bagasse using escherichia coli K011 |
| title_short | Bioethanol production from sugarcane bagasse using escherichia coli K011 |
| title_sort | bioethanol production from sugarcane bagasse using escherichia coli k011 |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/10834/ http://ir.unimas.my/id/eprint/10834/13/Amira%20Dayana%20Binti%20Abd.%20Rahman%2024pgs.pdf http://ir.unimas.my/id/eprint/10834/16/Amira%20Dayana%20Binti%20Abd.%20Rahman%20ft.pdf |