The use of random amplified polymorphic dna (RAPD) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem
A study was carried out to determine the genomic heterogeneity among fungi isolated from agarwood stem. A total of 17 fungus isolates with 13 unique species was obtained from the Virology Laboratory Unimas which made up of Trichoderma spirale, Trichoderma hamatum, Trichoderma hazianum, Hypocrea l...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10784/ http://ir.unimas.my/id/eprint/10784/1/Lim.pdf http://ir.unimas.my/id/eprint/10784/4/Lim%20Wei%20ft.pdf |
| _version_ | 1848836860299706368 |
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| author | Lim, Wei Yong |
| author_facet | Lim, Wei Yong |
| author_sort | Lim, Wei Yong |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A study was carried out to determine the genomic heterogeneity among fungi isolated from
agarwood stem. A total of 17 fungus isolates with 13 unique species was obtained from the
Virology Laboratory Unimas which made up of Trichoderma spirale, Trichoderma hamatum,
Trichoderma hazianum, Hypocrea lixii, Aspergillus niger, Aspergillus flavus, Aspergillus
/ubingensis, Curvularia ajJinis, Grammothele fuligo, Fusarium solani, Lasiodiplodia theobromae,
Wllitjordia scopulosa, and Penicillium citrinum. The stock cultures have been revived on the
freshly prepared nutrient media potato dextrose agar (PDA) and molecular identification of genetic
variability was performed by using the random amplified polymorphic DNA (RAPD) technology.
The RAPD-PCR fingerprint obtained by using primer OPA-14 outstood the primer (GTG)s with
comparatively high level of polymorphic DNA markers. Gel electrophoresis process was also
optimized to which better band patterns in terms of quality and quantity was observed. The
resulting band patterns were calculated for their' band scores from which the phylogenetic tree was
constructed. From the phylogenetic tree, four fungi namely Trichoderma hamatum, Trichoderma
hazianum, Hypocrea lixii, and Aspergillus niger have showed genomic heterogeneity. |
| first_indexed | 2025-11-15T06:30:28Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-10784 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:30:28Z |
| publishDate | 2015 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-107842024-02-20T07:48:16Z http://ir.unimas.my/id/eprint/10784/ The use of random amplified polymorphic dna (RAPD) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem Lim, Wei Yong S Agriculture (General) SB Plant culture A study was carried out to determine the genomic heterogeneity among fungi isolated from agarwood stem. A total of 17 fungus isolates with 13 unique species was obtained from the Virology Laboratory Unimas which made up of Trichoderma spirale, Trichoderma hamatum, Trichoderma hazianum, Hypocrea lixii, Aspergillus niger, Aspergillus flavus, Aspergillus /ubingensis, Curvularia ajJinis, Grammothele fuligo, Fusarium solani, Lasiodiplodia theobromae, Wllitjordia scopulosa, and Penicillium citrinum. The stock cultures have been revived on the freshly prepared nutrient media potato dextrose agar (PDA) and molecular identification of genetic variability was performed by using the random amplified polymorphic DNA (RAPD) technology. The RAPD-PCR fingerprint obtained by using primer OPA-14 outstood the primer (GTG)s with comparatively high level of polymorphic DNA markers. Gel electrophoresis process was also optimized to which better band patterns in terms of quality and quantity was observed. The resulting band patterns were calculated for their' band scores from which the phylogenetic tree was constructed. From the phylogenetic tree, four fungi namely Trichoderma hamatum, Trichoderma hazianum, Hypocrea lixii, and Aspergillus niger have showed genomic heterogeneity. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10784/1/Lim.pdf text en http://ir.unimas.my/id/eprint/10784/4/Lim%20Wei%20ft.pdf Lim, Wei Yong (2015) The use of random amplified polymorphic dna (RAPD) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | S Agriculture (General) SB Plant culture Lim, Wei Yong The use of random amplified polymorphic dna (RAPD) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem |
| title | The use of random amplified polymorphic dna (RAPD) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem |
| title_full | The use of random amplified polymorphic dna (RAPD) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem |
| title_fullStr | The use of random amplified polymorphic dna (RAPD) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem |
| title_full_unstemmed | The use of random amplified polymorphic dna (RAPD) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem |
| title_short | The use of random amplified polymorphic dna (RAPD) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem |
| title_sort | use of random amplified polymorphic dna (rapd) analysis to determine the genomic heterogeneity among the fungi isolated from agarwood stem |
| topic | S Agriculture (General) SB Plant culture |
| url | http://ir.unimas.my/id/eprint/10784/ http://ir.unimas.my/id/eprint/10784/1/Lim.pdf http://ir.unimas.my/id/eprint/10784/4/Lim%20Wei%20ft.pdf |