Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate
This research focuses on the characterization of fungi producing phenolic compound which is ferulic acid through solid-state fermentation using rice husk and empty fruit bunch as substrates. Screening of isolated fungi by using total phenolic content and feruloyl esterase assays showed that UMAS P2...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Online Access: | http://ir.unimas.my/id/eprint/10782/ http://ir.unimas.my/id/eprint/10782/4/Azharuddin%20full.pdf |
| _version_ | 1848836859665317888 |
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| author | Muhamad Azharuddin, Muhamad Nor |
| author_facet | Muhamad Azharuddin, Muhamad Nor |
| author_sort | Muhamad Azharuddin, Muhamad Nor |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | This research focuses on the characterization of fungi producing phenolic compound which is ferulic acid through solid-state fermentation using rice husk and empty fruit bunch as substrates. Screening of isolated fungi by using total phenolic content and feruloyl esterase
assays showed that UMAS P2 andUMAS B2 were able to produce phenolic compound which is ferulic acid. The morphological characteristic and molecular identification was done and the result showed that UMAS P2 is Rhizomucor variabilis and UMAS B2 is Fusarium chlamydosporum. Determination of the production of phenolic compound using Rhizomucor variabilis and Fusarium chlamydosporum under solid state fermentation is achieved by total phenolic content, 'while feruloyl esterase assays for the detection of fem lic acid. The production of phenolic compound by Rhizomucor variabilis under solid
state fermentation of rice husk and empty fruit bunch was recorded as 0.087 mglg and 0.156 mglg, respectively, while the production of phenolic compound by Fusarium
ch/amydosporum under solid state fermentation of rice husk and empty fruit bunch was 0.078 mglg and 0.065 mglg respectively. Besides, the feruloyl esterase activity by
Rhizomucor variabilis under solid state fermentation of rice husk and empty fruit bunch was recorded as 1.721 U/g and 0.597 U/g, while the feruloyl esterase activity by Fusarium chlamydosporum under solid state fermentation of rice husk and empty fruit bunch was 1.806 U/g and 1.024 U/g, respectively. In conclusion, Rhizomucor variabilis and Fusarium chlamydosporum have an ability to produce phenolic compound by utilizing agricultural waste such as rice husk and empty fruit bunch as carbon source. |
| first_indexed | 2025-11-15T06:30:28Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-10782 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:30:28Z |
| publishDate | 2015 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-107822023-08-18T09:06:22Z http://ir.unimas.my/id/eprint/10782/ Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate Muhamad Azharuddin, Muhamad Nor GE Environmental Sciences S Agriculture (General) This research focuses on the characterization of fungi producing phenolic compound which is ferulic acid through solid-state fermentation using rice husk and empty fruit bunch as substrates. Screening of isolated fungi by using total phenolic content and feruloyl esterase assays showed that UMAS P2 andUMAS B2 were able to produce phenolic compound which is ferulic acid. The morphological characteristic and molecular identification was done and the result showed that UMAS P2 is Rhizomucor variabilis and UMAS B2 is Fusarium chlamydosporum. Determination of the production of phenolic compound using Rhizomucor variabilis and Fusarium chlamydosporum under solid state fermentation is achieved by total phenolic content, 'while feruloyl esterase assays for the detection of fem lic acid. The production of phenolic compound by Rhizomucor variabilis under solid state fermentation of rice husk and empty fruit bunch was recorded as 0.087 mglg and 0.156 mglg, respectively, while the production of phenolic compound by Fusarium ch/amydosporum under solid state fermentation of rice husk and empty fruit bunch was 0.078 mglg and 0.065 mglg respectively. Besides, the feruloyl esterase activity by Rhizomucor variabilis under solid state fermentation of rice husk and empty fruit bunch was recorded as 1.721 U/g and 0.597 U/g, while the feruloyl esterase activity by Fusarium chlamydosporum under solid state fermentation of rice husk and empty fruit bunch was 1.806 U/g and 1.024 U/g, respectively. In conclusion, Rhizomucor variabilis and Fusarium chlamydosporum have an ability to produce phenolic compound by utilizing agricultural waste such as rice husk and empty fruit bunch as carbon source. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10782/4/Azharuddin%20full.pdf Muhamad Azharuddin, Muhamad Nor (2015) Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences S Agriculture (General) Muhamad Azharuddin, Muhamad Nor Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate |
| title | Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate |
| title_full | Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate |
| title_fullStr | Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate |
| title_full_unstemmed | Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate |
| title_short | Screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate |
| title_sort | screening, isolation and characterization of phenolic compound producing fungi using agricultural waste as substrate |
| topic | GE Environmental Sciences S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/10782/ http://ir.unimas.my/id/eprint/10782/4/Azharuddin%20full.pdf |