Production of Crude Xylanase from Aspergillus niger under Solid State Fermentation (SSF) by Using Different Agro Wastes as Substrates

Agro waste is a highly potential material that can be used as a substrate in solid state fermentation (SSF) for the production of enzyme. The problem caused by agro wastes can be reduced by manipulating them to produce xylanase which is one type of enzyme that provide numerous applications to ind...

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Bibliographic Details
Main Author: Ramizatulsafani, Ramli
Format: Final Year Project Report / IMRAD
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/10781/
http://ir.unimas.my/id/eprint/10781/1/Ramizatul.pdf
http://ir.unimas.my/id/eprint/10781/4/Ramizatulsafani%20ft.pdf
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Summary:Agro waste is a highly potential material that can be used as a substrate in solid state fermentation (SSF) for the production of enzyme. The problem caused by agro wastes can be reduced by manipulating them to produce xylanase which is one type of enzyme that provide numerous applications to industries. This study is focused on the production of crude xylanase from Aspergillus niger by using different types of agro wastes as substrates namely pineapple peels, rice husks and oil palm empty fruit bunches through SSF. The SSF parameters such as incubation period, initial moisture content of substrate and incubation temperature that affecting SSF to produce maximum enzyme production in term of enzyme activity were studied. Based on the results, it has found that the best substrate that produced the highest enzyme activity of 0.404 Vlml was pineapple peel with incubation for 8 days at 40°C by using initial moisture content of 70%.