Identification and characterisation of gut microflora of insectivorous bats from Sarawak

Bats are among the mammalian species that can serve as the host for zoonotic pathogens, like virus, fungi and bacteria. Therefore, it is important to identify and characterise the microbial diversity that reside within these natural host. The purpose of this project is due to the awareness that th...

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Bibliographic Details
Main Author: Clarice, M. Asinti
Format: Final Year Project Report / IMRAD
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/10777/
http://ir.unimas.my/id/eprint/10777/3/Clarice%20M.%20Asinti%20%2024pgs.pdf
http://ir.unimas.my/id/eprint/10777/6/Clarice%20M.%20Asinti%20%20ft.pdf
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author Clarice, M. Asinti
author_facet Clarice, M. Asinti
author_sort Clarice, M. Asinti
building UNIMAS Institutional Repository
collection Online Access
description Bats are among the mammalian species that can serve as the host for zoonotic pathogens, like virus, fungi and bacteria. Therefore, it is important to identify and characterise the microbial diversity that reside within these natural host. The purpose of this project is due to the awareness that the natural microflora of insectivorous bats that could potentially cause zoonotic disease to human. The identification and characterisation of the isolated gut microflora were conducted through biochemical tests analysis and molecular methods using PCR for DNA sequence analysis of 16S rRNA. The estimation of viable bacteria present in the gut of 16 bats through rectal swabbing ranged from 1.03xl04 to 1.49xl09 CFU/mL. A total of II genera of bacteria were identified from 97 isolates by using biochemical tests which were Bacillus, Escherichia, Klebsiella, Enterobacter, Acinetobacter Pseudomonas, Staphylococcus, Salmonella, Shigella, Serratia and Vibrio. A total of II species of bacteria from 14 isolates were identified through comparison of the sequences with BLAST, which were Clavibacter michiganensis, Enterococcus faecalis, Acinetobacter calcoaceticlls, A. nosocomialis, A. baumanii, Bacillus thuringiensis. B. cereus, Enterobacter cloacae, E. hormaechei. Staphylococcus scillri. and S. succinlls.
first_indexed 2025-11-15T06:30:26Z
format Final Year Project Report / IMRAD
id unimas-10777
institution Universiti Malaysia Sarawak
institution_category Local University
language English
English
last_indexed 2025-11-15T06:30:26Z
publishDate 2015
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-107772023-01-20T07:59:54Z http://ir.unimas.my/id/eprint/10777/ Identification and characterisation of gut microflora of insectivorous bats from Sarawak Clarice, M. Asinti QL Zoology QP Physiology Bats are among the mammalian species that can serve as the host for zoonotic pathogens, like virus, fungi and bacteria. Therefore, it is important to identify and characterise the microbial diversity that reside within these natural host. The purpose of this project is due to the awareness that the natural microflora of insectivorous bats that could potentially cause zoonotic disease to human. The identification and characterisation of the isolated gut microflora were conducted through biochemical tests analysis and molecular methods using PCR for DNA sequence analysis of 16S rRNA. The estimation of viable bacteria present in the gut of 16 bats through rectal swabbing ranged from 1.03xl04 to 1.49xl09 CFU/mL. A total of II genera of bacteria were identified from 97 isolates by using biochemical tests which were Bacillus, Escherichia, Klebsiella, Enterobacter, Acinetobacter Pseudomonas, Staphylococcus, Salmonella, Shigella, Serratia and Vibrio. A total of II species of bacteria from 14 isolates were identified through comparison of the sequences with BLAST, which were Clavibacter michiganensis, Enterococcus faecalis, Acinetobacter calcoaceticlls, A. nosocomialis, A. baumanii, Bacillus thuringiensis. B. cereus, Enterobacter cloacae, E. hormaechei. Staphylococcus scillri. and S. succinlls. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10777/3/Clarice%20M.%20Asinti%20%2024pgs.pdf text en http://ir.unimas.my/id/eprint/10777/6/Clarice%20M.%20Asinti%20%20ft.pdf Clarice, M. Asinti (2015) Identification and characterisation of gut microflora of insectivorous bats from Sarawak. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QL Zoology
QP Physiology
Clarice, M. Asinti
Identification and characterisation of gut microflora of insectivorous bats from Sarawak
title Identification and characterisation of gut microflora of insectivorous bats from Sarawak
title_full Identification and characterisation of gut microflora of insectivorous bats from Sarawak
title_fullStr Identification and characterisation of gut microflora of insectivorous bats from Sarawak
title_full_unstemmed Identification and characterisation of gut microflora of insectivorous bats from Sarawak
title_short Identification and characterisation of gut microflora of insectivorous bats from Sarawak
title_sort identification and characterisation of gut microflora of insectivorous bats from sarawak
topic QL Zoology
QP Physiology
url http://ir.unimas.my/id/eprint/10777/
http://ir.unimas.my/id/eprint/10777/3/Clarice%20M.%20Asinti%20%2024pgs.pdf
http://ir.unimas.my/id/eprint/10777/6/Clarice%20M.%20Asinti%20%20ft.pdf