Identification and characterisation of gut microflora of insectivorous bats from Sarawak
Bats are among the mammalian species that can serve as the host for zoonotic pathogens, like virus, fungi and bacteria. Therefore, it is important to identify and characterise the microbial diversity that reside within these natural host. The purpose of this project is due to the awareness that th...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Online Access: | http://ir.unimas.my/id/eprint/10777/ http://ir.unimas.my/id/eprint/10777/3/Clarice%20M.%20Asinti%20%2024pgs.pdf http://ir.unimas.my/id/eprint/10777/6/Clarice%20M.%20Asinti%20%20ft.pdf |
| Summary: | Bats are among the mammalian species that can serve as the host for zoonotic pathogens, like virus, fungi
and bacteria. Therefore, it is important to identify and characterise the microbial diversity that reside within
these natural host. The purpose of this project is due to the awareness that the natural microflora of
insectivorous bats that could potentially cause zoonotic disease to human. The identification and
characterisation of the isolated gut microflora were conducted through biochemical tests analysis and
molecular methods using PCR for DNA sequence analysis of 16S rRNA. The estimation of viable bacteria
present in the gut of 16 bats through rectal swabbing ranged from 1.03xl04 to 1.49xl09 CFU/mL. A total of
II genera of bacteria were identified from 97 isolates by using biochemical tests which were Bacillus,
Escherichia, Klebsiella, Enterobacter, Acinetobacter Pseudomonas, Staphylococcus, Salmonella, Shigella,
Serratia and Vibrio. A total of II species of bacteria from 14 isolates were identified through comparison of
the sequences with BLAST, which were Clavibacter michiganensis, Enterococcus faecalis, Acinetobacter
calcoaceticlls, A. nosocomialis, A. baumanii, Bacillus thuringiensis. B. cereus, Enterobacter cloacae, E.
hormaechei. Staphylococcus scillri. and S. succinlls. |
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