Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol

Bioethanol is a very environmentally friendly liquid biofuel that is not only renewable, but also sustainable. It is currently deemed as a highly suitable additive and substitute energy source to replace fossil based fuel. In this study, bioethanol was produced from sago hampas by using commercial a...

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Main Authors: Micky, Vincent, Berry Rence, Senawi, Ennry, Esut, Norizawati, Muhammad Nor, Dayang Salwani, Awang Adeni
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/10729/
http://ir.unimas.my/id/eprint/10729/1/Sequential.pdf
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author Micky, Vincent
Berry Rence, Senawi
Ennry, Esut
Norizawati, Muhammad Nor
Dayang Salwani, Awang Adeni
author_facet Micky, Vincent
Berry Rence, Senawi
Ennry, Esut
Norizawati, Muhammad Nor
Dayang Salwani, Awang Adeni
author_sort Micky, Vincent
building UNIMAS Institutional Repository
collection Online Access
description Bioethanol is a very environmentally friendly liquid biofuel that is not only renewable, but also sustainable. It is currently deemed as a highly suitable additive and substitute energy source to replace fossil based fuel. In this study, bioethanol was produced from sago hampas by using commercial amylase, cellulase and Saccharomyces cerevisiae via sequential saccharification and simultaneous fermentation (SSSF), a modified version of the simultaneous saccharification and fermentation (SSF) process. SSSF was performed on sago hampas at 2.5 and 5.0% (w/v) feedstock load for five days. The samples taken from the SSSF broths were analysed via high performance liquid chromatography (HPLC) for ethanol, glucose and acetic acid production. From the results obtained, SSSF with 5.0% sago hampas loading exhibited the highest ethanol production at 14.13 g/L (77.43% of theoretical ethanol yield), while SSSF using 2.5% sago hampas loading produced ethanol at 6.45 g/L (69.24% of theoretical ethanol yield). This study has shown that ethanol not only can be produced from sago hampas using different enzyme mixtures and S. cerevisiae via SSSF, but yields were also high, making this process highly promising for the production of cheap and sustainable ethanol as fuel.
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spelling unimas-107292022-01-28T02:22:20Z http://ir.unimas.my/id/eprint/10729/ Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol Micky, Vincent Berry Rence, Senawi Ennry, Esut Norizawati, Muhammad Nor Dayang Salwani, Awang Adeni GE Environmental Sciences Bioethanol is a very environmentally friendly liquid biofuel that is not only renewable, but also sustainable. It is currently deemed as a highly suitable additive and substitute energy source to replace fossil based fuel. In this study, bioethanol was produced from sago hampas by using commercial amylase, cellulase and Saccharomyces cerevisiae via sequential saccharification and simultaneous fermentation (SSSF), a modified version of the simultaneous saccharification and fermentation (SSF) process. SSSF was performed on sago hampas at 2.5 and 5.0% (w/v) feedstock load for five days. The samples taken from the SSSF broths were analysed via high performance liquid chromatography (HPLC) for ethanol, glucose and acetic acid production. From the results obtained, SSSF with 5.0% sago hampas loading exhibited the highest ethanol production at 14.13 g/L (77.43% of theoretical ethanol yield), while SSSF using 2.5% sago hampas loading produced ethanol at 6.45 g/L (69.24% of theoretical ethanol yield). This study has shown that ethanol not only can be produced from sago hampas using different enzyme mixtures and S. cerevisiae via SSSF, but yields were also high, making this process highly promising for the production of cheap and sustainable ethanol as fuel. Universiti Kebangsaan Malaysia 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10729/1/Sequential.pdf Micky, Vincent and Berry Rence, Senawi and Ennry, Esut and Norizawati, Muhammad Nor and Dayang Salwani, Awang Adeni (2015) Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol. Sains Malaysiana, 44 (6). pp. 899-904. ISSN 0126-6039 https://scholar.google.com.my/citations?view_op=view_citation&hl=en&user=bky9s3cAAAAJ&citation_for_view=bky9s3cAAAAJ:3fE2CSJIrl8C
spellingShingle GE Environmental Sciences
Micky, Vincent
Berry Rence, Senawi
Ennry, Esut
Norizawati, Muhammad Nor
Dayang Salwani, Awang Adeni
Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol
title Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol
title_full Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol
title_fullStr Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol
title_full_unstemmed Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol
title_short Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol
title_sort sequential saccharification and simultaneous fermentation (sssf) of sago hampas for the production of bioethanol
topic GE Environmental Sciences
url http://ir.unimas.my/id/eprint/10729/
http://ir.unimas.my/id/eprint/10729/
http://ir.unimas.my/id/eprint/10729/1/Sequential.pdf