Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA
An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrialencoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed...
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| Format: | Article |
| Language: | English |
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Springer Japan
2015
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| Online Access: | http://ir.unimas.my/id/eprint/10719/ http://ir.unimas.my/id/eprint/10719/1/NO%2099%20Pseudo%E2%80%91nitzschia%20species%20%28Bacillariophyceae%29%20identification%20%28abstract%29.pdf |
| _version_ | 1848836845170851840 |
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| author | Sing, Tung Teng |
| author_facet | Sing, Tung Teng |
| author_sort | Sing, Tung Teng |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | An attempt was made to infer the phylogeny
of Pseudo-nitzschia species by using the mitochondrialencoded
gene, cytochrome c oxidase subunit I (cox1),
and comparing it with the nuclear-encoded large subunit
ribosomal DNA (LSU rDNA). A pair of primers targeting
Pseudo-nitzschia cox1 was designed in silico and used to
infer the molecular phylogeny of Pseudo-nitzschia. The
primer pair was tested using genomic DNAs isolated from
six species of Pseudo-nitzschia from Malaysia. The phylogenetic
inference of cox1 was then compared to the LSU
rDNA phylogeny. Phylogenetic reconstructions of both
data sets revealed monophyly of Pseudo-nitzschia species
complexes. The range of genetic divergences among
Pseudo-nitzschia species were higher in the cox1 data set
(3.5–20.4 %) compared to the LSU rDNA data set (0.1–
8.8 %). The present study suggests that high genetic divergence
in cox1 of Pseudo-nitzschia species could be a useful
genetic marker for DNA bar coding. |
| first_indexed | 2025-11-15T06:30:14Z |
| format | Article |
| id | unimas-10719 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:30:14Z |
| publishDate | 2015 |
| publisher | Springer Japan |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-107192016-10-21T06:42:07Z http://ir.unimas.my/id/eprint/10719/ Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA Sing, Tung Teng SH Aquaculture. Fisheries. Angling An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrialencoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed in silico and used to infer the molecular phylogeny of Pseudo-nitzschia. The primer pair was tested using genomic DNAs isolated from six species of Pseudo-nitzschia from Malaysia. The phylogenetic inference of cox1 was then compared to the LSU rDNA phylogeny. Phylogenetic reconstructions of both data sets revealed monophyly of Pseudo-nitzschia species complexes. The range of genetic divergences among Pseudo-nitzschia species were higher in the cox1 data set (3.5–20.4 %) compared to the LSU rDNA data set (0.1– 8.8 %). The present study suggests that high genetic divergence in cox1 of Pseudo-nitzschia species could be a useful genetic marker for DNA bar coding. Springer Japan 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10719/1/NO%2099%20Pseudo%E2%80%91nitzschia%20species%20%28Bacillariophyceae%29%20identification%20%28abstract%29.pdf Sing, Tung Teng (2015) Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA. Fisheries Science, 2015 (81). pp. 831-838. ISSN 1444-2906 http://link.springer.com/article/10.1007%2Fs12562-015-0902-7 DOI : 10.1007/s12562-015-0902-7 |
| spellingShingle | SH Aquaculture. Fisheries. Angling Sing, Tung Teng Pseudo‑nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA |
| title | Pseudo‑nitzschia species (Bacillariophyceae) identification
and delineation using mitochondrial cox1 gene sequences
as compared to LSU rDNA |
| title_full | Pseudo‑nitzschia species (Bacillariophyceae) identification
and delineation using mitochondrial cox1 gene sequences
as compared to LSU rDNA |
| title_fullStr | Pseudo‑nitzschia species (Bacillariophyceae) identification
and delineation using mitochondrial cox1 gene sequences
as compared to LSU rDNA |
| title_full_unstemmed | Pseudo‑nitzschia species (Bacillariophyceae) identification
and delineation using mitochondrial cox1 gene sequences
as compared to LSU rDNA |
| title_short | Pseudo‑nitzschia species (Bacillariophyceae) identification
and delineation using mitochondrial cox1 gene sequences
as compared to LSU rDNA |
| title_sort | pseudo‑nitzschia species (bacillariophyceae) identification
and delineation using mitochondrial cox1 gene sequences
as compared to lsu rdna |
| topic | SH Aquaculture. Fisheries. Angling |
| url | http://ir.unimas.my/id/eprint/10719/ http://ir.unimas.my/id/eprint/10719/ http://ir.unimas.my/id/eprint/10719/ http://ir.unimas.my/id/eprint/10719/1/NO%2099%20Pseudo%E2%80%91nitzschia%20species%20%28Bacillariophyceae%29%20identification%20%28abstract%29.pdf |