Paclitaxel Inhibits Expression of Neuronal Nitric Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion
AIm: This study evaluated the neuroprotective effect of intrathecally infused paclitaxel in the prevention of motoneuron death and mitochondrial dysfunction following brachial plexus avulsion injury. Mat erIal and Methods: Brachial root avulsion injury was induced in Sprague-Dawley rats. The Pacli...
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| Format: | Article |
| Language: | English |
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Turkish Neurosurgery, ITN
2015
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| Online Access: | http://ir.unimas.my/id/eprint/10709/ http://ir.unimas.my/id/eprint/10709/1/Abdul%20Aziz.pdf |
| _version_ | 1848836842943676416 |
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| author | Sim, Sze Kiat Tan, Yew Chin Tee, Jong Huat Abdul Aziz, Yusoff Jafri Malin, Abdullah |
| author_facet | Sim, Sze Kiat Tan, Yew Chin Tee, Jong Huat Abdul Aziz, Yusoff Jafri Malin, Abdullah |
| author_sort | Sim, Sze Kiat |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | AIm: This study evaluated the neuroprotective effect of intrathecally infused paclitaxel in the prevention of motoneuron death and
mitochondrial dysfunction following brachial plexus avulsion injury.
Mat erIal and Methods: Brachial root avulsion injury was induced in Sprague-Dawley rats. The Paclitaxel treatment group (n = 32) received
a 5-d intrathecal infusion of paclitaxel (256 ng/d) via a micro infusion pump, whereas the Control group (n = 32) received normal saline. The
cervical cord was harvested at survival times of 1, 2, 4, and 6 wk (n = 8 each). The number of surviving and nNOS-positive motoneurons at the
injury level in the ventral horn was determined with NADPH-d histochemistry. Mitochondrial function at this location was measured with CcO
histochemistry and densitometry. An independent t-test was applied to detect differences between the study groups at specific survival times.
Result s: The Paclitaxel treatment group showed a significant relative reduction in nNOS expression at 2, 4, and 6 wk, and significantly
improved mitochondrial function at 4 and 6 wk. Motoneuron survival was significantly increased at 2, 4, and 6 wk.
ConclusIon: Paclitaxel has a significant neuroprotective effect against spinal motoneuron degeneration following brachial plexus avulsion
injury, which involves inhibition of nNOS expression and prevention of mitochondrial dysfunction. |
| first_indexed | 2025-11-15T06:30:12Z |
| format | Article |
| id | unimas-10709 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:30:12Z |
| publishDate | 2015 |
| publisher | Turkish Neurosurgery, ITN |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-107092021-06-22T14:39:45Z http://ir.unimas.my/id/eprint/10709/ Paclitaxel Inhibits Expression of Neuronal Nitric Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion Sim, Sze Kiat Tan, Yew Chin Tee, Jong Huat Abdul Aziz, Yusoff Jafri Malin, Abdullah Q Science (General) R Medicine (General) AIm: This study evaluated the neuroprotective effect of intrathecally infused paclitaxel in the prevention of motoneuron death and mitochondrial dysfunction following brachial plexus avulsion injury. Mat erIal and Methods: Brachial root avulsion injury was induced in Sprague-Dawley rats. The Paclitaxel treatment group (n = 32) received a 5-d intrathecal infusion of paclitaxel (256 ng/d) via a micro infusion pump, whereas the Control group (n = 32) received normal saline. The cervical cord was harvested at survival times of 1, 2, 4, and 6 wk (n = 8 each). The number of surviving and nNOS-positive motoneurons at the injury level in the ventral horn was determined with NADPH-d histochemistry. Mitochondrial function at this location was measured with CcO histochemistry and densitometry. An independent t-test was applied to detect differences between the study groups at specific survival times. Result s: The Paclitaxel treatment group showed a significant relative reduction in nNOS expression at 2, 4, and 6 wk, and significantly improved mitochondrial function at 4 and 6 wk. Motoneuron survival was significantly increased at 2, 4, and 6 wk. ConclusIon: Paclitaxel has a significant neuroprotective effect against spinal motoneuron degeneration following brachial plexus avulsion injury, which involves inhibition of nNOS expression and prevention of mitochondrial dysfunction. Turkish Neurosurgery, ITN 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10709/1/Abdul%20Aziz.pdf Sim, Sze Kiat and Tan, Yew Chin and Tee, Jong Huat and Abdul Aziz, Yusoff and Jafri Malin, Abdullah (2015) Paclitaxel Inhibits Expression of Neuronal Nitric Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion. Turk Neurosurg, 25 (4). pp. 617-624. ISSN 1019-5149 http://www.scopus.com/inward/record.url?eid=2-s2.0-84938376732&partnerID=40&md5=eb041973ba1971b9b6c0fac6f4853707 doi: 10.5137/1019-5149.JTN.14035-15.1 |
| spellingShingle | Q Science (General) R Medicine (General) Sim, Sze Kiat Tan, Yew Chin Tee, Jong Huat Abdul Aziz, Yusoff Jafri Malin, Abdullah Paclitaxel Inhibits Expression of Neuronal Nitric Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion |
| title | Paclitaxel Inhibits Expression of Neuronal Nitric
Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion |
| title_full | Paclitaxel Inhibits Expression of Neuronal Nitric
Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion |
| title_fullStr | Paclitaxel Inhibits Expression of Neuronal Nitric
Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion |
| title_full_unstemmed | Paclitaxel Inhibits Expression of Neuronal Nitric
Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion |
| title_short | Paclitaxel Inhibits Expression of Neuronal Nitric
Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion |
| title_sort | paclitaxel inhibits expression of neuronal nitric
oxide synthase and prevents mitochondrial dysfunction in spinal ventral horn in rats after c7 spinal root avulsion |
| topic | Q Science (General) R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/10709/ http://ir.unimas.my/id/eprint/10709/ http://ir.unimas.my/id/eprint/10709/ http://ir.unimas.my/id/eprint/10709/1/Abdul%20Aziz.pdf |