Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish
The present study was conducted to assess the rapid molecular identification and characterization of 45 Vibrio parahaemolyticus isolates from 15 samples of 3 different types of fish (Kembung, Bawal and Sangeh) in the Kuching-Samarahan district. Polymerase chain reaction (PCR) based confirmation w...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Published: |
Universiti Putra Malaysia
2015
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10706/ |
| _version_ | 1848836842007298048 |
|---|---|
| author | Vincent, M. Chan, CSW Kasing, Apun |
| author_facet | Vincent, M. Chan, CSW Kasing, Apun |
| author_sort | Vincent, M. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The present study was conducted to assess the rapid molecular identification and characterization
of 45 Vibrio parahaemolyticus isolates from 15 samples of 3 different types of fish (Kembung,
Bawal and Sangeh) in the Kuching-Samarahan district. Polymerase chain reaction (PCR) based
confirmation was done targeting the 450 bp fragment of the thermolabile (tl) gene, while DNA
fingerprinting was performed using Randomly Amplified Polymorphic DNA (RAPD) PCR
with the primer GEN15008. All the 45 V. parahaemolyticus isolates were positive for the tl
gene, however, only 34 were typable via RAPD-PCR with bands sizes ranging from slightly
over 250 bp to 2.5 kbp. The degree of diversity was then determined via the Simpson Index
which showed a value of 0.891, indicating high diversity among the isolates. Data from the
RAPD-PCR fingerprints were later used to construct a dendrogram for clustal analysis. From
the dendrogram, the 34 isolates were grouped into 2 major clusters containing 26 and 8 isolates,
respectively. Further analyses of the dendrogram also indicated that the 34 isolated were
clustered according to the period of sampling. This is an interesting observation as it shows the
high discriminatory capability of RAPD-PCR to be used as molecular epidemiological tool to
study the temporal distribution of V. parahaemolyticus. |
| first_indexed | 2025-11-15T06:30:11Z |
| format | Article |
| id | unimas-10706 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| last_indexed | 2025-11-15T06:30:11Z |
| publishDate | 2015 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-107062023-07-31T03:50:16Z http://ir.unimas.my/id/eprint/10706/ Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish Vincent, M. Chan, CSW Kasing, Apun GE Environmental Sciences The present study was conducted to assess the rapid molecular identification and characterization of 45 Vibrio parahaemolyticus isolates from 15 samples of 3 different types of fish (Kembung, Bawal and Sangeh) in the Kuching-Samarahan district. Polymerase chain reaction (PCR) based confirmation was done targeting the 450 bp fragment of the thermolabile (tl) gene, while DNA fingerprinting was performed using Randomly Amplified Polymorphic DNA (RAPD) PCR with the primer GEN15008. All the 45 V. parahaemolyticus isolates were positive for the tl gene, however, only 34 were typable via RAPD-PCR with bands sizes ranging from slightly over 250 bp to 2.5 kbp. The degree of diversity was then determined via the Simpson Index which showed a value of 0.891, indicating high diversity among the isolates. Data from the RAPD-PCR fingerprints were later used to construct a dendrogram for clustal analysis. From the dendrogram, the 34 isolates were grouped into 2 major clusters containing 26 and 8 isolates, respectively. Further analyses of the dendrogram also indicated that the 34 isolated were clustered according to the period of sampling. This is an interesting observation as it shows the high discriminatory capability of RAPD-PCR to be used as molecular epidemiological tool to study the temporal distribution of V. parahaemolyticus. Universiti Putra Malaysia 2015 Article PeerReviewed Vincent, M. and Chan, CSW and Kasing, Apun (2015) Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish. International Food Research Journal, 22 (4). pp. 1705-1710. ISSN 2231-7546 https://scholar.google.es/citations?view_op=view_citation&hl=ca&user=bky9s3cAAAAJ&citation_for_view=bky9s3cAAAAJ:MXK_kJrjxJIC |
| spellingShingle | GE Environmental Sciences Vincent, M. Chan, CSW Kasing, Apun Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish |
| title | Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish |
| title_full | Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish |
| title_fullStr | Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish |
| title_full_unstemmed | Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish |
| title_short | Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish |
| title_sort | molecular confirmation and characterization of vibrio parahaemolyticus from retailed fish |
| topic | GE Environmental Sciences |
| url | http://ir.unimas.my/id/eprint/10706/ http://ir.unimas.my/id/eprint/10706/ |