Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus
Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Trade Science Inc.
2015
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| Online Access: | http://ir.unimas.my/id/eprint/10669/ http://ir.unimas.my/id/eprint/10669/1/Expression.pdf |
| _version_ | 1848836830707843072 |
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| author | Henry Sum, Magdline Sia Andrew, Anna |
| author_facet | Henry Sum, Magdline Sia Andrew, Anna |
| author_sort | Henry Sum, Magdline Sia |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system.
The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of purification. The expression of the 24-kDa recombinant protein was detected by SDS-PAGE and the protein reactivity was evaluated by western blot analysis. The immunogenicity of the 24 kDa proteinwas further tested against human positive and negative sera for chikungunya and dengue. The results showed that the recombinant antigen was able to detect CHIKV positive sera and no cross reactivity was observed with dengue virus positive serum |
| first_indexed | 2025-11-15T06:30:00Z |
| format | Article |
| id | unimas-10669 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:30:00Z |
| publishDate | 2015 |
| publisher | Trade Science Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-106692022-01-26T08:15:40Z http://ir.unimas.my/id/eprint/10669/ Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus Henry Sum, Magdline Sia Andrew, Anna Q Science (General) QR355 Virology R Medicine (General) Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of purification. The expression of the 24-kDa recombinant protein was detected by SDS-PAGE and the protein reactivity was evaluated by western blot analysis. The immunogenicity of the 24 kDa proteinwas further tested against human positive and negative sera for chikungunya and dengue. The results showed that the recombinant antigen was able to detect CHIKV positive sera and no cross reactivity was observed with dengue virus positive serum Trade Science Inc. 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10669/1/Expression.pdf Henry Sum, Magdline Sia and Andrew, Anna (2015) Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus. Bio Technology - An Indian Journal, 11 (3). pp. 109-114. ISSN 0974-7435 |
| spellingShingle | Q Science (General) QR355 Virology R Medicine (General) Henry Sum, Magdline Sia Andrew, Anna Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_full | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_fullStr | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_full_unstemmed | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_short | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_sort | expression and evaluation of a 24-kda recombinant protein of the n-terminal e2 glycoprotein of chikungunya virus |
| topic | Q Science (General) QR355 Virology R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/10669/ http://ir.unimas.my/id/eprint/10669/1/Expression.pdf |