Over expression of Recombinant CP3 Protein of Rice Tungro Spherical Virus in Prokaryotic Expression System
Currently, serological based diagnosis of rice tungro disease (RTD) is being limited by the availability of antisera. This study aims to produce recombinant protein from the coat protein (CP)3 of rice tungro spherical virus (RTSV) as a potential antigen in producing high titre antibodies for use...
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2014
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| Online Access: | http://ir.unimas.my/id/eprint/10668/ http://ir.unimas.my/id/eprint/10668/1/Over%20expression%20of%20Recombinant%20CP3%20%28abstract%29.pdf |
| _version_ | 1848836830403756032 |
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| author | Yee, Siew Fung Chu, Chia Huey Henry Sum, Magdline Sia |
| author_facet | Yee, Siew Fung Chu, Chia Huey Henry Sum, Magdline Sia |
| author_sort | Yee, Siew Fung |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Currently, serological based diagnosis of rice tungro disease (RTD) is being limited by the
availability of antisera. This study aims to produce recombinant protein from the coat protein
(CP)3 of rice tungro spherical virus (RTSV) as a potential antigen in producing high titre
antibodies for use in detection of RTD. Amplified RTSV CP3 gene of approximately 900 bp
was cloned into pETSUMO vector. The recombinant plasmid was transformed into cloning host
Mach1™-T1® E.coli cells and screened for recombinant gene in the correct orientation. Plasmid
DNA from positive transformants was isolated and transformed into expression host BL21
(DE3) E.coli cells. One positive clone was selected for large scale expression where the
expressed protein was purified and analysed by SDS-PAGE and Western blot. This
recombinant RTSV CP3 protein, 46 kDa in molecular mass with 6x His-tag and SUMO protein
fused to its N-terminal, was found to be antigenic when it reacted with our in-house generated
polyclonal antibodies against tungro viruses. |
| first_indexed | 2025-11-15T06:30:00Z |
| format | Article |
| id | unimas-10668 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:30:00Z |
| publishDate | 2014 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-106682016-03-01T02:25:30Z http://ir.unimas.my/id/eprint/10668/ Over expression of Recombinant CP3 Protein of Rice Tungro Spherical Virus in Prokaryotic Expression System Yee, Siew Fung Chu, Chia Huey Henry Sum, Magdline Sia Q Science (General) QR355 Virology S Agriculture (General) Currently, serological based diagnosis of rice tungro disease (RTD) is being limited by the availability of antisera. This study aims to produce recombinant protein from the coat protein (CP)3 of rice tungro spherical virus (RTSV) as a potential antigen in producing high titre antibodies for use in detection of RTD. Amplified RTSV CP3 gene of approximately 900 bp was cloned into pETSUMO vector. The recombinant plasmid was transformed into cloning host Mach1™-T1® E.coli cells and screened for recombinant gene in the correct orientation. Plasmid DNA from positive transformants was isolated and transformed into expression host BL21 (DE3) E.coli cells. One positive clone was selected for large scale expression where the expressed protein was purified and analysed by SDS-PAGE and Western blot. This recombinant RTSV CP3 protein, 46 kDa in molecular mass with 6x His-tag and SUMO protein fused to its N-terminal, was found to be antigenic when it reacted with our in-house generated polyclonal antibodies against tungro viruses. Hibiscus Publisher 2014-12-14 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10668/1/Over%20expression%20of%20Recombinant%20CP3%20%28abstract%29.pdf Yee, Siew Fung and Chu, Chia Huey and Henry Sum, Magdline Sia (2014) Over expression of Recombinant CP3 Protein of Rice Tungro Spherical Virus in Prokaryotic Expression System. Bioremediation Science & Technology Research, 2 (2). pp. 38-42. ISSN e-ISSN 2289-5892 http://journal.hibiscuspublisher.com/index.php/BSTR/article/view/159 |
| spellingShingle | Q Science (General) QR355 Virology S Agriculture (General) Yee, Siew Fung Chu, Chia Huey Henry Sum, Magdline Sia Over expression of Recombinant CP3 Protein of Rice Tungro Spherical Virus in Prokaryotic Expression System |
| title | Over expression of Recombinant CP3 Protein of Rice Tungro Spherical Virus in Prokaryotic Expression System |
| title_full | Over expression of Recombinant CP3 Protein of Rice Tungro Spherical Virus in Prokaryotic Expression System |
| title_fullStr | Over expression of Recombinant CP3 Protein of Rice Tungro Spherical Virus in Prokaryotic Expression System |
| title_full_unstemmed | Over expression of Recombinant CP3 Protein of Rice Tungro Spherical Virus in Prokaryotic Expression System |
| title_short | Over expression of Recombinant CP3 Protein of Rice Tungro Spherical Virus in Prokaryotic Expression System |
| title_sort | over expression of recombinant cp3 protein of rice tungro spherical virus in prokaryotic expression system |
| topic | Q Science (General) QR355 Virology S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/10668/ http://ir.unimas.my/id/eprint/10668/ http://ir.unimas.my/id/eprint/10668/1/Over%20expression%20of%20Recombinant%20CP3%20%28abstract%29.pdf |