Mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses

A recently isolated lactic acid bacteria (LAB) from the Biochemistry Laboratory of UNIMAS that is capable to produce lactic acid (LA) from carbon source by fermentation under high temperature was studied. The strain was used for LA production from molasses at 20 g/l, 40 g/l and 60 g/l substrate conc...

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Main Author: Thian, Sing Kit
Format: Final Year Project Report / IMRAD
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/10660/
http://ir.unimas.my/id/eprint/10660/1/Mathematical%20Modelling%20On%20The%20Growth%20of%20Thermophilic%20Lactic%20Acid%20Bacteria%20In%20Molasses%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/10660/8/Thian%20Sing%20Kit.pdf
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author Thian, Sing Kit
author_facet Thian, Sing Kit
author_sort Thian, Sing Kit
building UNIMAS Institutional Repository
collection Online Access
description A recently isolated lactic acid bacteria (LAB) from the Biochemistry Laboratory of UNIMAS that is capable to produce lactic acid (LA) from carbon source by fermentation under high temperature was studied. The strain was used for LA production from molasses at 20 g/l, 40 g/l and 60 g/l substrate concentration under controlled pH at 6.0 and temperature at 45 °C. Molasses is an abundant and low-cost bio-resources from the sugar mill industry and contain fermentable sugars. In this study, a mathematical model was generated to describe the batch fermentation of molasses to LA with the LAB strain, with the bacterial density, LA production and residual sugar as responses. The Monod equation was applied to describe the bacterial growth, while the Luedeking-Piret equation was employed to describe the production of LA. The fermentations produced a maximum specific growth rate, μmax of 0.381 h-1 and substrate saturation constant of 13.81 g/l. The model depicted that the production of LA from molasses medium was related to the rate of bacterial growth and the quantity of bacteria present. The model could be used to study and optimize the growth and production of lactic acid using the LAB in molasses medium fermentation system.
first_indexed 2025-11-15T06:29:58Z
format Final Year Project Report / IMRAD
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
English
last_indexed 2025-11-15T06:29:58Z
publishDate 2015
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-106602024-04-01T07:23:52Z http://ir.unimas.my/id/eprint/10660/ Mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses Thian, Sing Kit Q Science (General) A recently isolated lactic acid bacteria (LAB) from the Biochemistry Laboratory of UNIMAS that is capable to produce lactic acid (LA) from carbon source by fermentation under high temperature was studied. The strain was used for LA production from molasses at 20 g/l, 40 g/l and 60 g/l substrate concentration under controlled pH at 6.0 and temperature at 45 °C. Molasses is an abundant and low-cost bio-resources from the sugar mill industry and contain fermentable sugars. In this study, a mathematical model was generated to describe the batch fermentation of molasses to LA with the LAB strain, with the bacterial density, LA production and residual sugar as responses. The Monod equation was applied to describe the bacterial growth, while the Luedeking-Piret equation was employed to describe the production of LA. The fermentations produced a maximum specific growth rate, μmax of 0.381 h-1 and substrate saturation constant of 13.81 g/l. The model depicted that the production of LA from molasses medium was related to the rate of bacterial growth and the quantity of bacteria present. The model could be used to study and optimize the growth and production of lactic acid using the LAB in molasses medium fermentation system. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10660/1/Mathematical%20Modelling%20On%20The%20Growth%20of%20Thermophilic%20Lactic%20Acid%20Bacteria%20In%20Molasses%20%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/10660/8/Thian%20Sing%20Kit.pdf Thian, Sing Kit (2015) Mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle Q Science (General)
Thian, Sing Kit
Mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses
title Mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses
title_full Mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses
title_fullStr Mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses
title_full_unstemmed Mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses
title_short Mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses
title_sort mathematical modelling on the growth of thermophilic lactic acid bacteria in molasses
topic Q Science (General)
url http://ir.unimas.my/id/eprint/10660/
http://ir.unimas.my/id/eprint/10660/1/Mathematical%20Modelling%20On%20The%20Growth%20of%20Thermophilic%20Lactic%20Acid%20Bacteria%20In%20Molasses%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/10660/8/Thian%20Sing%20Kit.pdf