Detection of bacillus cereus from imported rice in Malaysia
Bacillus cereus is frequently associated with foodborne illness outbreak. The common food vehicles for transmission of B. cereus are rice, rice products and starchy foods. Hence, it is crucial to investigate the biosafety of B. cereus in rice. The bacteria were enumerated by performing Most Probabl...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10649/ http://ir.unimas.my/id/eprint/10649/1/Pei%20Shze%20%2824pgs_.pdf http://ir.unimas.my/id/eprint/10649/4/Tan%20Pei%20Shze%20ft.pdf |
| _version_ | 1848836825623298048 |
|---|---|
| author | Tan, Pei Shze |
| author_facet | Tan, Pei Shze |
| author_sort | Tan, Pei Shze |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Bacillus cereus is frequently associated with foodborne illness outbreak. The common food vehicles for
transmission of B. cereus are rice, rice products and starchy foods. Hence, it is crucial to investigate the biosafety of B. cereus in rice. The bacteria were enumerated by performing Most Probable Number (MPN) approach
followed by Polymerase Chain Reaction (PCR) Assay targeting gyraseB (gyrB) gene at 475 bp. A total of 20
imported raw rice samples from various countries were evaluated. The finding indicated that the occurrence of B.
cereus was >1100 MPN/g for all the imported raw rice samples. In addition, 100% of the imported raw rice
samples were detected with presence of B. cereus. The result highlights the potential risk of B. cereus to cause
food poisoning in rice. Hence, extra precautions in preparing, handling and storage of the rice are necessary to
prevent microbial contamination. |
| first_indexed | 2025-11-15T06:29:55Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-10649 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:29:55Z |
| publishDate | 2015 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-106492024-04-01T06:28:20Z http://ir.unimas.my/id/eprint/10649/ Detection of bacillus cereus from imported rice in Malaysia Tan, Pei Shze Q Science (General) Bacillus cereus is frequently associated with foodborne illness outbreak. The common food vehicles for transmission of B. cereus are rice, rice products and starchy foods. Hence, it is crucial to investigate the biosafety of B. cereus in rice. The bacteria were enumerated by performing Most Probable Number (MPN) approach followed by Polymerase Chain Reaction (PCR) Assay targeting gyraseB (gyrB) gene at 475 bp. A total of 20 imported raw rice samples from various countries were evaluated. The finding indicated that the occurrence of B. cereus was >1100 MPN/g for all the imported raw rice samples. In addition, 100% of the imported raw rice samples were detected with presence of B. cereus. The result highlights the potential risk of B. cereus to cause food poisoning in rice. Hence, extra precautions in preparing, handling and storage of the rice are necessary to prevent microbial contamination. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10649/1/Pei%20Shze%20%2824pgs_.pdf text en http://ir.unimas.my/id/eprint/10649/4/Tan%20Pei%20Shze%20ft.pdf Tan, Pei Shze (2015) Detection of bacillus cereus from imported rice in Malaysia. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) Tan, Pei Shze Detection of bacillus cereus from imported rice in Malaysia |
| title | Detection of bacillus cereus from imported rice in Malaysia |
| title_full | Detection of bacillus cereus from imported rice in Malaysia |
| title_fullStr | Detection of bacillus cereus from imported rice in Malaysia |
| title_full_unstemmed | Detection of bacillus cereus from imported rice in Malaysia |
| title_short | Detection of bacillus cereus from imported rice in Malaysia |
| title_sort | detection of bacillus cereus from imported rice in malaysia |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/10649/ http://ir.unimas.my/id/eprint/10649/1/Pei%20Shze%20%2824pgs_.pdf http://ir.unimas.my/id/eprint/10649/4/Tan%20Pei%20Shze%20ft.pdf |