Screening and identification of microbes with potential antagonistic activity against phytophthora capsici
Phytophthora capsid infection on the black pepper can affect the production of black pepper. This infection can be managed using fungicides such as metalaxyl, copper oxychloride and phosphonate. However, the use of these fungicides against the infection can cause damage to the environment. Thus, it...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10632/ http://ir.unimas.my/id/eprint/10632/1/Tang%2824pgs%29.pdf http://ir.unimas.my/id/eprint/10632/4/Tang%20Lok%20Ing%20ft.pdf |
| _version_ | 1848836823459037184 |
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| author | Tang, Lok Ing |
| author_facet | Tang, Lok Ing |
| author_sort | Tang, Lok Ing |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Phytophthora capsid infection on the black pepper can affect the production of black pepper. This infection can be managed using fungicides such as metalaxyl, copper oxychloride and phosphonate. However, the use of these fungicides against the infection can cause damage to the environment. Thus, it is important to look for a new approach to control P. capsid. The aims of this study are to isolate and characterize the bacteria that are antagonism against P. capsid. In this study, the bacteria were screened for their antagonistic activity through spore germination test and double plate assay. Hence, KDKBB83 and SB (2)6 that showed high antagonistic activity was selected for molecular characterization. The mo lecular characterization was done by using 16S rRNA sequence analysis. Analysis of 16S rRNA sequencing suggested the closest identities of the bacterial isolates as Serratia marcescens and Pseudomonas aeruginosa by using Basic Local Alignment Search Tool (BLAST) via National Center for Biotechnology Information (NCBI). |
| first_indexed | 2025-11-15T06:29:53Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-10632 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:29:53Z |
| publishDate | 2015 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-106322024-04-01T04:56:38Z http://ir.unimas.my/id/eprint/10632/ Screening and identification of microbes with potential antagonistic activity against phytophthora capsici Tang, Lok Ing QR Microbiology Phytophthora capsid infection on the black pepper can affect the production of black pepper. This infection can be managed using fungicides such as metalaxyl, copper oxychloride and phosphonate. However, the use of these fungicides against the infection can cause damage to the environment. Thus, it is important to look for a new approach to control P. capsid. The aims of this study are to isolate and characterize the bacteria that are antagonism against P. capsid. In this study, the bacteria were screened for their antagonistic activity through spore germination test and double plate assay. Hence, KDKBB83 and SB (2)6 that showed high antagonistic activity was selected for molecular characterization. The mo lecular characterization was done by using 16S rRNA sequence analysis. Analysis of 16S rRNA sequencing suggested the closest identities of the bacterial isolates as Serratia marcescens and Pseudomonas aeruginosa by using Basic Local Alignment Search Tool (BLAST) via National Center for Biotechnology Information (NCBI). Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10632/1/Tang%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/10632/4/Tang%20Lok%20Ing%20ft.pdf Tang, Lok Ing (2015) Screening and identification of microbes with potential antagonistic activity against phytophthora capsici. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QR Microbiology Tang, Lok Ing Screening and identification of microbes with potential antagonistic activity against phytophthora capsici |
| title | Screening and identification of microbes with potential antagonistic activity against phytophthora capsici |
| title_full | Screening and identification of microbes with potential antagonistic activity against phytophthora capsici |
| title_fullStr | Screening and identification of microbes with potential antagonistic activity against phytophthora capsici |
| title_full_unstemmed | Screening and identification of microbes with potential antagonistic activity against phytophthora capsici |
| title_short | Screening and identification of microbes with potential antagonistic activity against phytophthora capsici |
| title_sort | screening and identification of microbes with potential antagonistic activity against phytophthora capsici |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/10632/ http://ir.unimas.my/id/eprint/10632/1/Tang%2824pgs%29.pdf http://ir.unimas.my/id/eprint/10632/4/Tang%20Lok%20Ing%20ft.pdf |