Isolation and cloning of ABCA1 gene from Rasbora sarawakensis

ATP-binding cassette (ABC) genes is a gene that encodes ABC transporter proteins that has vital functions in A TP hydrolysis. The genes also play a role in the maintenance of the plasma membrane and in the transport of fatty acids and sterols within the body. In this research, the objectives is to c...

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Main Author: Kuba, Annie
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/10622/
http://ir.unimas.my/id/eprint/10622/2/Annie.pdf
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author Kuba, Annie
author_facet Kuba, Annie
author_sort Kuba, Annie
building UNIMAS Institutional Repository
collection Online Access
description ATP-binding cassette (ABC) genes is a gene that encodes ABC transporter proteins that has vital functions in A TP hydrolysis. The genes also play a role in the maintenance of the plasma membrane and in the transport of fatty acids and sterols within the body. In this research, the objectives is to clone and identify ABCAI gene from Rasbora sarawakensis, and to establish the ABCA 1 gene transcript in the Rasbora sarawakensis for the gene expression used. The polymerase reaction chain was performed by using degenerate primer and was transform by usingE.coli XLI-Blue competent cell. Therefore, through this research it can help to improve and understand the physiological function of ABCAI gene in R. sara wakens is.
first_indexed 2025-11-15T06:29:51Z
format Final Year Project Report / IMRAD
id unimas-10622
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:29:51Z
publishDate 2015
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-106222023-08-07T06:42:47Z http://ir.unimas.my/id/eprint/10622/ Isolation and cloning of ABCA1 gene from Rasbora sarawakensis Kuba, Annie Q Science (General) ATP-binding cassette (ABC) genes is a gene that encodes ABC transporter proteins that has vital functions in A TP hydrolysis. The genes also play a role in the maintenance of the plasma membrane and in the transport of fatty acids and sterols within the body. In this research, the objectives is to clone and identify ABCAI gene from Rasbora sarawakensis, and to establish the ABCA 1 gene transcript in the Rasbora sarawakensis for the gene expression used. The polymerase reaction chain was performed by using degenerate primer and was transform by usingE.coli XLI-Blue competent cell. Therefore, through this research it can help to improve and understand the physiological function of ABCAI gene in R. sara wakens is. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10622/2/Annie.pdf Kuba, Annie (2015) Isolation and cloning of ABCA1 gene from Rasbora sarawakensis. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle Q Science (General)
Kuba, Annie
Isolation and cloning of ABCA1 gene from Rasbora sarawakensis
title Isolation and cloning of ABCA1 gene from Rasbora sarawakensis
title_full Isolation and cloning of ABCA1 gene from Rasbora sarawakensis
title_fullStr Isolation and cloning of ABCA1 gene from Rasbora sarawakensis
title_full_unstemmed Isolation and cloning of ABCA1 gene from Rasbora sarawakensis
title_short Isolation and cloning of ABCA1 gene from Rasbora sarawakensis
title_sort isolation and cloning of abca1 gene from rasbora sarawakensis
topic Q Science (General)
url http://ir.unimas.my/id/eprint/10622/
http://ir.unimas.my/id/eprint/10622/2/Annie.pdf