Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei
Partial and non-host resistances to rust fungi in barley (Hordeum vulgare) may be based on pathogen-associated molecular pattern (PAMP)-triggered immunity. Understanding partial resistance may help to understand non-host resistance, and vice versa. We constructed two non-gridded BAC libraries from c...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Berlin Heidelberg
2016
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10601/ http://ir.unimas.my/id/eprint/10601/7/wang.pdf |
| _version_ | 1848836817357373440 |
|---|---|
| author | Yeo, F. K. S. |
| author_facet | Yeo, F. K. S. |
| author_sort | Yeo, F. K. S. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Partial and non-host resistances to rust fungi in barley (Hordeum vulgare) may be based on pathogen-associated molecular pattern (PAMP)-triggered immunity. Understanding partial resistance may help to understand non-host resistance, and vice versa. We constructed two non-gridded BAC libraries from cultivar Vada and line SusPtrit. Vada is immune to non-adapted Puccinia rust fungi, and partially resistant to P. hordei. SusPtrit is susceptible to several non-adapted rust fungi, and has been used for mapping QTLs for non-host and partial resistance. The BAC libraries help to identify genes determining the natural variation for partial and non-host resistances of barley to rust fungi. A major-effect QTL, Rphq2, for partial resistance to P. hordei was mapped in a complete Vada and an incomplete SusPtrit contig. The physical distance between the markers flanking Rphq2 was 195 Kbp in Vada and at least 226 Kbp in SusPtrit. This marker interval was predicted to contain 12 genes in either accession, of which only five genes were in common. The haplotypes represented by Vada and SusPtrit were found in 57 and 43 %, respectively, of a 194 barley accessions panel. The lack of homology between the two haplotypes probably explains the suppression of recombination in the Rphq2 area and limit further genetic resolution in fine mapping. The possible candidate genes for Rphq2 encode peroxidases, kinases and a member of seven-in-absentia protein family. This result suggests that Rphq2 does not belong to the NB-LRR gene family and does not resemble any of the partial resistance genes cloned previously. |
| first_indexed | 2025-11-15T06:29:47Z |
| format | Article |
| id | unimas-10601 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:29:47Z |
| publishDate | 2016 |
| publisher | Springer Berlin Heidelberg |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-106012021-05-21T12:13:31Z http://ir.unimas.my/id/eprint/10601/ Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei Yeo, F. K. S. SB Plant culture Partial and non-host resistances to rust fungi in barley (Hordeum vulgare) may be based on pathogen-associated molecular pattern (PAMP)-triggered immunity. Understanding partial resistance may help to understand non-host resistance, and vice versa. We constructed two non-gridded BAC libraries from cultivar Vada and line SusPtrit. Vada is immune to non-adapted Puccinia rust fungi, and partially resistant to P. hordei. SusPtrit is susceptible to several non-adapted rust fungi, and has been used for mapping QTLs for non-host and partial resistance. The BAC libraries help to identify genes determining the natural variation for partial and non-host resistances of barley to rust fungi. A major-effect QTL, Rphq2, for partial resistance to P. hordei was mapped in a complete Vada and an incomplete SusPtrit contig. The physical distance between the markers flanking Rphq2 was 195 Kbp in Vada and at least 226 Kbp in SusPtrit. This marker interval was predicted to contain 12 genes in either accession, of which only five genes were in common. The haplotypes represented by Vada and SusPtrit were found in 57 and 43 %, respectively, of a 194 barley accessions panel. The lack of homology between the two haplotypes probably explains the suppression of recombination in the Rphq2 area and limit further genetic resolution in fine mapping. The possible candidate genes for Rphq2 encode peroxidases, kinases and a member of seven-in-absentia protein family. This result suggests that Rphq2 does not belong to the NB-LRR gene family and does not resemble any of the partial resistance genes cloned previously. Springer Berlin Heidelberg 2016 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10601/7/wang.pdf Yeo, F. K. S. (2016) Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei. Theoretical and Applied Genetics, 129 (2). pp. 289-304. ISSN 1432-2242 http://link.springer.com/article/10.1007%2Fs00122-015-2627-5 DOI : 10.1007/s00122-015-2627-5 |
| spellingShingle | SB Plant culture Yeo, F. K. S. Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei |
| title | Haplotype divergence and multiple candidate genes at Rphq2,
a partial resistance QTL of barley to Puccinia hordei |
| title_full | Haplotype divergence and multiple candidate genes at Rphq2,
a partial resistance QTL of barley to Puccinia hordei |
| title_fullStr | Haplotype divergence and multiple candidate genes at Rphq2,
a partial resistance QTL of barley to Puccinia hordei |
| title_full_unstemmed | Haplotype divergence and multiple candidate genes at Rphq2,
a partial resistance QTL of barley to Puccinia hordei |
| title_short | Haplotype divergence and multiple candidate genes at Rphq2,
a partial resistance QTL of barley to Puccinia hordei |
| title_sort | haplotype divergence and multiple candidate genes at rphq2,
a partial resistance qtl of barley to puccinia hordei |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/10601/ http://ir.unimas.my/id/eprint/10601/ http://ir.unimas.my/id/eprint/10601/ http://ir.unimas.my/id/eprint/10601/7/wang.pdf |