Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source

The Gram-positive rhizobacterium, Bacillus amyloliquefaciens have the ability to produce bioactive metabolite to suppress numerous plant fungal diseases. The present study was performed to find out which carbon source, glucose, maltose and sucrose are able to aids the production of bioactive metabol...

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Main Author: Akmal Athirah, binti Taifor
Format: Final Year Project Report / IMRAD
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/10527/
http://ir.unimas.my/id/eprint/10527/3/Akmal%20Athirah%20binti%20Taifor%2024pgs.pdf
http://ir.unimas.my/id/eprint/10527/6/Akmal%20Athirah%20binti%20Taifor%20ft.pdf
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author Akmal Athirah, binti Taifor
author_facet Akmal Athirah, binti Taifor
author_sort Akmal Athirah, binti Taifor
building UNIMAS Institutional Repository
collection Online Access
description The Gram-positive rhizobacterium, Bacillus amyloliquefaciens have the ability to produce bioactive metabolite to suppress numerous plant fungal diseases. The present study was performed to find out which carbon source, glucose, maltose and sucrose are able to aids the production of bioactive metabolite by the antagonistic bacteria, B. amyloliquefaciens in vitro against three plant pathogenic fungi, Colletotrichum gloeosporioides, Fusarium solani and Phytophthora capsici in dual culture assay. The results showed that after day 7 of dual culture, maltose is showing the highest percentage of inhibition against C. gloeosporioides and P. capsici that are 19.05% and 90.91%, respectively while sucrose has the highest percentage of inhibition against F. solani at 27.14%. However, when the B. amyloliquefaciens with maltose and sucrose was assayed on those enzyme assays such as amylase, cellulose, chitinase and protease, the results showed that B. amyloliquefaciens with sucrose as carbon source had more activity as compared to maltose. Therefore, these shows that sucrose is a potential carbon source for the production of bioactive metabolite of B. amyloliquefaciens against plant pathogenic fungi.
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format Final Year Project Report / IMRAD
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
English
last_indexed 2025-11-15T06:29:32Z
publishDate 2015
publisher Universiti Malaysia Sarawak, (UNIMAS)
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spelling unimas-105272023-02-13T08:56:54Z http://ir.unimas.my/id/eprint/10527/ Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source Akmal Athirah, binti Taifor Q Science (General) The Gram-positive rhizobacterium, Bacillus amyloliquefaciens have the ability to produce bioactive metabolite to suppress numerous plant fungal diseases. The present study was performed to find out which carbon source, glucose, maltose and sucrose are able to aids the production of bioactive metabolite by the antagonistic bacteria, B. amyloliquefaciens in vitro against three plant pathogenic fungi, Colletotrichum gloeosporioides, Fusarium solani and Phytophthora capsici in dual culture assay. The results showed that after day 7 of dual culture, maltose is showing the highest percentage of inhibition against C. gloeosporioides and P. capsici that are 19.05% and 90.91%, respectively while sucrose has the highest percentage of inhibition against F. solani at 27.14%. However, when the B. amyloliquefaciens with maltose and sucrose was assayed on those enzyme assays such as amylase, cellulose, chitinase and protease, the results showed that B. amyloliquefaciens with sucrose as carbon source had more activity as compared to maltose. Therefore, these shows that sucrose is a potential carbon source for the production of bioactive metabolite of B. amyloliquefaciens against plant pathogenic fungi. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10527/3/Akmal%20Athirah%20binti%20Taifor%2024pgs.pdf text en http://ir.unimas.my/id/eprint/10527/6/Akmal%20Athirah%20binti%20Taifor%20ft.pdf Akmal Athirah, binti Taifor (2015) Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle Q Science (General)
Akmal Athirah, binti Taifor
Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source
title Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source
title_full Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source
title_fullStr Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source
title_full_unstemmed Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source
title_short Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source
title_sort production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source
topic Q Science (General)
url http://ir.unimas.my/id/eprint/10527/
http://ir.unimas.my/id/eprint/10527/3/Akmal%20Athirah%20binti%20Taifor%2024pgs.pdf
http://ir.unimas.my/id/eprint/10527/6/Akmal%20Athirah%20binti%20Taifor%20ft.pdf