Comparison of the growth of all four dengue virus serotypes in C6/36 and vero cell lines

Dengue virus, which is transmitted by an infected Aedes aegypti mosquito, is one of the most rampant arboviruses that have been infecting millions of people around the world. Previous studies showed that the virus propagated in different cell lines showed diverse results in different experiments con...

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Main Author: Rahmah Fadilah, Sjam’un
Format: Final Year Project Report / IMRAD
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
Subjects:
Online Access:http://ir.unimas.my/id/eprint/10413/
http://ir.unimas.my/id/eprint/10413/1/Rahmah.pdf
http://ir.unimas.my/id/eprint/10413/4/Rahmah%20Fadilah%20ft.pdf
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author Rahmah Fadilah, Sjam’un
author_facet Rahmah Fadilah, Sjam’un
author_sort Rahmah Fadilah, Sjam’un
building UNIMAS Institutional Repository
collection Online Access
description Dengue virus, which is transmitted by an infected Aedes aegypti mosquito, is one of the most rampant arboviruses that have been infecting millions of people around the world. Previous studies showed that the virus propagated in different cell lines showed diverse results in different experiments conducted. Hence, the aim of this study is to compare the growth of all four dengue virus serotypes in both C6/36 and Vero cell lines. Three main parameters that were observed and analysed were the differences in cytopathic effect, virus titers and RNA concentration obtained from the experiment. The results obtained showed that the growth of DENV was significantly higher in C6/36 cell line compared to Vero cell line for all four DENV serotypes. Hence, this study allowed a better understanding on the susceptibility differences that occurred where high rate of viral propagated indicated a high affinity of the cell line towards dengue virus.
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format Final Year Project Report / IMRAD
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
English
last_indexed 2025-11-15T06:29:09Z
publishDate 2015
publisher Universiti Malaysia Sarawak, (UNIMAS)
recordtype eprints
repository_type Digital Repository
spelling unimas-104132024-03-13T05:43:37Z http://ir.unimas.my/id/eprint/10413/ Comparison of the growth of all four dengue virus serotypes in C6/36 and vero cell lines Rahmah Fadilah, Sjam’un QR Microbiology Dengue virus, which is transmitted by an infected Aedes aegypti mosquito, is one of the most rampant arboviruses that have been infecting millions of people around the world. Previous studies showed that the virus propagated in different cell lines showed diverse results in different experiments conducted. Hence, the aim of this study is to compare the growth of all four dengue virus serotypes in both C6/36 and Vero cell lines. Three main parameters that were observed and analysed were the differences in cytopathic effect, virus titers and RNA concentration obtained from the experiment. The results obtained showed that the growth of DENV was significantly higher in C6/36 cell line compared to Vero cell line for all four DENV serotypes. Hence, this study allowed a better understanding on the susceptibility differences that occurred where high rate of viral propagated indicated a high affinity of the cell line towards dengue virus. Universiti Malaysia Sarawak, (UNIMAS) 2015 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/10413/1/Rahmah.pdf text en http://ir.unimas.my/id/eprint/10413/4/Rahmah%20Fadilah%20ft.pdf Rahmah Fadilah, Sjam’un (2015) Comparison of the growth of all four dengue virus serotypes in C6/36 and vero cell lines. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QR Microbiology
Rahmah Fadilah, Sjam’un
Comparison of the growth of all four dengue virus serotypes in C6/36 and vero cell lines
title Comparison of the growth of all four dengue virus serotypes in C6/36 and vero cell lines
title_full Comparison of the growth of all four dengue virus serotypes in C6/36 and vero cell lines
title_fullStr Comparison of the growth of all four dengue virus serotypes in C6/36 and vero cell lines
title_full_unstemmed Comparison of the growth of all four dengue virus serotypes in C6/36 and vero cell lines
title_short Comparison of the growth of all four dengue virus serotypes in C6/36 and vero cell lines
title_sort comparison of the growth of all four dengue virus serotypes in c6/36 and vero cell lines
topic QR Microbiology
url http://ir.unimas.my/id/eprint/10413/
http://ir.unimas.my/id/eprint/10413/1/Rahmah.pdf
http://ir.unimas.my/id/eprint/10413/4/Rahmah%20Fadilah%20ft.pdf