A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae)
A new species of Pseudo-nitzschia (Bacillariophyceae) is described from plankton samples collected from Port Dickson (Malacca Strait, Malaysia) and Manzanillo Bay (Colima, Mexico). The species possesses a distinctive falcate cell valve, from which they form sickle-like colonies in both environm...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Phycological Society of America
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10307/ http://ir.unimas.my/id/eprint/10307/1/Maria.pdf |
| _version_ | 1848836751480586240 |
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| author | Sing, Tung Teng Po, Teen Lim Hong, Chang Lim Maria, Rivera-Vilarelle Sonia, Quijano-Scheggia Yoshinobu, Takata Michael A., Quilliam Matthias, Wolf Stephen S., Bates Chui, Pin Leaw |
| author_facet | Sing, Tung Teng Po, Teen Lim Hong, Chang Lim Maria, Rivera-Vilarelle Sonia, Quijano-Scheggia Yoshinobu, Takata Michael A., Quilliam Matthias, Wolf Stephen S., Bates Chui, Pin Leaw |
| author_sort | Sing, Tung Teng |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A new species of Pseudo-nitzschia (Bacillariophyceae)
is described from plankton samples collected from
Port Dickson (Malacca Strait, Malaysia) and
Manzanillo Bay (Colima, Mexico). The species
possesses a distinctive falcate cell valve, from which
they form sickle-like colonies in both environmental
samples and cultured strains. Detailed observation of
frustules under TEM revealed ultrastructure that
closely resembles P. decipiens, yet the new species
differs by the valve shape and greater ranges of
striae and poroid densities. The species is readily
distinguished from the curve-shaped P. subcurvata
by the presence of a central interspace. The
morphological distinction is further supported by
phylogenetic discrimination. We sequenced and
analyzed the nuclear ribosomal RNA genes in the LSU
and the second internal transcribed spacer, including
its secondary structure, to infer the phylogenetic
relationship of the new species with its closest
relatives. The results revealed a distinct lineage of the
new species, forming a sister cluster with its related
species, P. decipiens and P. galaxiae, but not with
P. subcurvata. We examined the domoic acid (DA)
production of five cultured strains from Malaysia by
Liquid chromatography-mass spectrometry (LC-MS),
but they showed no detectable DA. Here, we present
the taxonomic description of the vegetative cells,
document the sexual reproduction, and detail the
molecular phylogenetics of Pseudo-nitzschia sabit sp. nov. |
| first_indexed | 2025-11-15T06:28:45Z |
| format | Article |
| id | unimas-10307 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:28:45Z |
| publishDate | 2015 |
| publisher | Phycological Society of America |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-103072021-06-22T14:19:50Z http://ir.unimas.my/id/eprint/10307/ A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae) Sing, Tung Teng Po, Teen Lim Hong, Chang Lim Maria, Rivera-Vilarelle Sonia, Quijano-Scheggia Yoshinobu, Takata Michael A., Quilliam Matthias, Wolf Stephen S., Bates Chui, Pin Leaw SH Aquaculture. Fisheries. Angling A new species of Pseudo-nitzschia (Bacillariophyceae) is described from plankton samples collected from Port Dickson (Malacca Strait, Malaysia) and Manzanillo Bay (Colima, Mexico). The species possesses a distinctive falcate cell valve, from which they form sickle-like colonies in both environmental samples and cultured strains. Detailed observation of frustules under TEM revealed ultrastructure that closely resembles P. decipiens, yet the new species differs by the valve shape and greater ranges of striae and poroid densities. The species is readily distinguished from the curve-shaped P. subcurvata by the presence of a central interspace. The morphological distinction is further supported by phylogenetic discrimination. We sequenced and analyzed the nuclear ribosomal RNA genes in the LSU and the second internal transcribed spacer, including its secondary structure, to infer the phylogenetic relationship of the new species with its closest relatives. The results revealed a distinct lineage of the new species, forming a sister cluster with its related species, P. decipiens and P. galaxiae, but not with P. subcurvata. We examined the domoic acid (DA) production of five cultured strains from Malaysia by Liquid chromatography-mass spectrometry (LC-MS), but they showed no detectable DA. Here, we present the taxonomic description of the vegetative cells, document the sexual reproduction, and detail the molecular phylogenetics of Pseudo-nitzschia sabit sp. nov. Phycological Society of America 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10307/1/Maria.pdf Sing, Tung Teng and Po, Teen Lim and Hong, Chang Lim and Maria, Rivera-Vilarelle and Sonia, Quijano-Scheggia and Yoshinobu, Takata and Michael A., Quilliam and Matthias, Wolf and Stephen S., Bates and Chui, Pin Leaw (2015) A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae). Journal of Phycology, 51 (4). pp. 706-725. ISSN 1529-8817 http://www.scopus.com/inward/record.url?eid=2-s2.0-84938904545&partnerID=40&md5=0ea0760929ed1d025454224a468ba615 DOI: 10.1111/jpy.12313 |
| spellingShingle | SH Aquaculture. Fisheries. Angling Sing, Tung Teng Po, Teen Lim Hong, Chang Lim Maria, Rivera-Vilarelle Sonia, Quijano-Scheggia Yoshinobu, Takata Michael A., Quilliam Matthias, Wolf Stephen S., Bates Chui, Pin Leaw A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae) |
| title | A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae) |
| title_full | A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae) |
| title_fullStr | A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae) |
| title_full_unstemmed | A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae) |
| title_short | A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae) |
| title_sort | non-toxigenic but morphologically and phylogenetically distinct new species of pseudo-nitzschia, p. sabit sp. nov. bacillariophyceae) |
| topic | SH Aquaculture. Fisheries. Angling |
| url | http://ir.unimas.my/id/eprint/10307/ http://ir.unimas.my/id/eprint/10307/ http://ir.unimas.my/id/eprint/10307/ http://ir.unimas.my/id/eprint/10307/1/Maria.pdf |