Development and polymorphism of simple sequence repeats (SSRs) in Kelampayan(Neolamarckia cadamba–Rubiaceae) using ISSR suppression PCR method
Simple sequence repeat (SSR) marker is a polymerase chain reaction (PCR)-based marker system which has become a marker of choice for understanding plant genetic diversity and a powerful tool in addressing genetic resources questions. However, the availability of SSR markers especially for forest t...
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| Format: | Article |
| Language: | English |
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Scholars Research Library
2014
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| Online Access: | http://ir.unimas.my/id/eprint/10222/ http://ir.unimas.my/id/eprint/10222/1/Phui.pdf |
| _version_ | 1848836735020040192 |
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| author | Seng-Loi, Phui Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah |
| author_facet | Seng-Loi, Phui Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah |
| author_sort | Seng-Loi, Phui |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Simple sequence repeat (SSR) marker is a polymerase chain reaction (PCR)-based marker system which has become
a marker of choice for understanding plant genetic diversity and a powerful tool in addressing genetic resources
questions. However, the availability of SSR markers especially for forest tree species is limited thus far due to the
high development cost, labour-intensive and time-consuming. The present study aimed to develop an array of SSR
markers for Neolamarckia cadamba using inter-simple sequence repeat (ISSR) suppression PCR method and further
assessed the polymorphisms and transferability of the markers to other species.In total, 15 out of 31 SSR markers
specific for N.cadamba were successfully developed and further characterized and validated by using 30 individuals
of N. cadamba. The markers exhibited a considerable high level of polymorphism across the tested N. cadamba
genotypes whereby 66 alleles were detected with an average of four alleles per locus. Most of the SSRloci analyzed
showed high polymorphism as indicated by their PIC value which was above 0.5. The most polymorphic lociwere:
NCAC11 (PIC=0.849), NCAC12 (PIC=0.722) and NCAG01 (PIC=0.712).The transferability rate was ranging from
26.7% to 73.3% among the four cross-genera species tested. The present study is the first report on the development
of SSR markers in N. cadamba. These markers provide valuable genomic resources that could pave the way for
exploiting SSR genotype data for effective selection of plus trees, provenance trials and establishment of forest seed
production areas (SPAs) of N. cadamba in the selected forest areas dedicated to planted forest development, and
molecular breeding of N. cadamba and other indigenous tropical tree species in future. |
| first_indexed | 2025-11-15T06:28:29Z |
| format | Article |
| id | unimas-10222 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:28:29Z |
| publishDate | 2014 |
| publisher | Scholars Research Library |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-102222021-06-29T17:23:54Z http://ir.unimas.my/id/eprint/10222/ Development and polymorphism of simple sequence repeats (SSRs) in Kelampayan(Neolamarckia cadamba–Rubiaceae) using ISSR suppression PCR method Seng-Loi, Phui Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah SB Plant culture Simple sequence repeat (SSR) marker is a polymerase chain reaction (PCR)-based marker system which has become a marker of choice for understanding plant genetic diversity and a powerful tool in addressing genetic resources questions. However, the availability of SSR markers especially for forest tree species is limited thus far due to the high development cost, labour-intensive and time-consuming. The present study aimed to develop an array of SSR markers for Neolamarckia cadamba using inter-simple sequence repeat (ISSR) suppression PCR method and further assessed the polymorphisms and transferability of the markers to other species.In total, 15 out of 31 SSR markers specific for N.cadamba were successfully developed and further characterized and validated by using 30 individuals of N. cadamba. The markers exhibited a considerable high level of polymorphism across the tested N. cadamba genotypes whereby 66 alleles were detected with an average of four alleles per locus. Most of the SSRloci analyzed showed high polymorphism as indicated by their PIC value which was above 0.5. The most polymorphic lociwere: NCAC11 (PIC=0.849), NCAC12 (PIC=0.722) and NCAG01 (PIC=0.712).The transferability rate was ranging from 26.7% to 73.3% among the four cross-genera species tested. The present study is the first report on the development of SSR markers in N. cadamba. These markers provide valuable genomic resources that could pave the way for exploiting SSR genotype data for effective selection of plus trees, provenance trials and establishment of forest seed production areas (SPAs) of N. cadamba in the selected forest areas dedicated to planted forest development, and molecular breeding of N. cadamba and other indigenous tropical tree species in future. Scholars Research Library 2014 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/10222/1/Phui.pdf Seng-Loi, Phui and Wei-Seng, Ho and Shek-Ling, Pang and Julaihi, Abdullah (2014) Development and polymorphism of simple sequence repeats (SSRs) in Kelampayan(Neolamarckia cadamba–Rubiaceae) using ISSR suppression PCR method. Archives of Applied Science Research, 6 (4). pp. 209-218. ISSN 0975-508X https://scholar.google.com/citations?view_op=view_citation&hl=en&user=VAl1VU8AAAAJ&citation_for_view=VAl1VU8AAAAJ:GFxP56DSvIMC |
| spellingShingle | SB Plant culture Seng-Loi, Phui Wei-Seng, Ho Shek-Ling, Pang Julaihi, Abdullah Development and polymorphism of simple sequence repeats (SSRs) in Kelampayan(Neolamarckia cadamba–Rubiaceae) using ISSR suppression PCR method |
| title | Development and polymorphism of simple sequence repeats (SSRs) in Kelampayan(Neolamarckia cadamba–Rubiaceae) using ISSR suppression PCR method |
| title_full | Development and polymorphism of simple sequence repeats (SSRs) in Kelampayan(Neolamarckia cadamba–Rubiaceae) using ISSR suppression PCR method |
| title_fullStr | Development and polymorphism of simple sequence repeats (SSRs) in Kelampayan(Neolamarckia cadamba–Rubiaceae) using ISSR suppression PCR method |
| title_full_unstemmed | Development and polymorphism of simple sequence repeats (SSRs) in Kelampayan(Neolamarckia cadamba–Rubiaceae) using ISSR suppression PCR method |
| title_short | Development and polymorphism of simple sequence repeats (SSRs) in Kelampayan(Neolamarckia cadamba–Rubiaceae) using ISSR suppression PCR method |
| title_sort | development and polymorphism of simple sequence repeats (ssrs) in kelampayan(neolamarckia cadamba–rubiaceae) using issr suppression pcr method |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/10222/ http://ir.unimas.my/id/eprint/10222/ http://ir.unimas.my/id/eprint/10222/1/Phui.pdf |