Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment
Sago palm (Metroxylon sagu) is a perennial plant native to Southeast Asia and exploited mainly for the starch content in its trunk. Genetic improvement of sago palm is extremely slow when compared to other annual starch crops. Urgent attention is needed to improve the sago palm planting material a...
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| Format: | Article |
| Language: | English |
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Hidawi Publishing Coperation
2014
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| Online Access: | http://ir.unimas.my/id/eprint/10164/ http://ir.unimas.my/id/eprint/10164/1/Genetic%20Transformation%20of%20Metroxylon%20sagu%20%28Rottb.%29%20Cultures%20via%20Agrobacterium-Mediated%20and%20Particle%20Bombardment%20%28abstract%29.pdf |
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| author | Evra Raunie, Ibrahim Md. Anowar, Hossain Hairul, Azman Roslan |
| author_facet | Evra Raunie, Ibrahim Md. Anowar, Hossain Hairul, Azman Roslan |
| author_sort | Evra Raunie, Ibrahim |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Sago palm (Metroxylon sagu) is a perennial plant native to Southeast Asia and exploited mainly for the starch content in its
trunk. Genetic improvement of sago palm is extremely slow when compared to other annual starch crops. Urgent attention is
needed to improve the sago palm planting material and can be achieved through nonconventional methods. We have previously
developed a tissue culture method for sago palm, which is used to provide the planting materials and to develop a genetic
transformation procedure. Here, we report the genetic transformation of sago embryonic callus derived from suspension culture
usingAgrobacteriumtumefaciens and gene gun systems.Thetransformed embryoids cellswere selected against Basta (concentration
10 to 30mg/L). Evidence of foreign genes integration and function of the bar and gus genes were verified via gene specific PCR
amplification, gus staining, and dot blot analysis.This study showed that the embryogenic callus was the most suitable material for
transformation as compared to the fine callus, embryoid stage, and initiated shoots.The gene gun transformation showed higher
transformation efficiency than the ones transformed using Agrobacteriumwhen targetswere bombarded once or twice using 280 psi
of helium pressure at 6 to 8 cm distance. |
| first_indexed | 2025-11-15T06:28:14Z |
| format | Article |
| id | unimas-10164 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:28:14Z |
| publishDate | 2014 |
| publisher | Hidawi Publishing Coperation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-101642016-01-13T01:09:42Z http://ir.unimas.my/id/eprint/10164/ Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment Evra Raunie, Ibrahim Md. Anowar, Hossain Hairul, Azman Roslan SB Plant culture Sago palm (Metroxylon sagu) is a perennial plant native to Southeast Asia and exploited mainly for the starch content in its trunk. Genetic improvement of sago palm is extremely slow when compared to other annual starch crops. Urgent attention is needed to improve the sago palm planting material and can be achieved through nonconventional methods. We have previously developed a tissue culture method for sago palm, which is used to provide the planting materials and to develop a genetic transformation procedure. Here, we report the genetic transformation of sago embryonic callus derived from suspension culture usingAgrobacteriumtumefaciens and gene gun systems.Thetransformed embryoids cellswere selected against Basta (concentration 10 to 30mg/L). Evidence of foreign genes integration and function of the bar and gus genes were verified via gene specific PCR amplification, gus staining, and dot blot analysis.This study showed that the embryogenic callus was the most suitable material for transformation as compared to the fine callus, embryoid stage, and initiated shoots.The gene gun transformation showed higher transformation efficiency than the ones transformed using Agrobacteriumwhen targetswere bombarded once or twice using 280 psi of helium pressure at 6 to 8 cm distance. Hidawi Publishing Coperation 2014 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10164/1/Genetic%20Transformation%20of%20Metroxylon%20sagu%20%28Rottb.%29%20Cultures%20via%20Agrobacterium-Mediated%20and%20Particle%20Bombardment%20%28abstract%29.pdf Evra Raunie, Ibrahim and Md. Anowar, Hossain and Hairul, Azman Roslan (2014) Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment. BioMed Research International, 2014. ISSN 695283 https://www.researchgate.net/publication/265088363_Genetic_Transformation_of_Metroxylon_sagu_Rottb_Cultures_via_Agrobacterium-Mediated_and_Particle_Bombardment http://dx.doi.org/10.1155/2014/348140 |
| spellingShingle | SB Plant culture Evra Raunie, Ibrahim Md. Anowar, Hossain Hairul, Azman Roslan Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment |
| title | Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment |
| title_full | Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment |
| title_fullStr | Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment |
| title_full_unstemmed | Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment |
| title_short | Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment |
| title_sort | genetic transformation of metroxylon sagu (rottb.) cultures via agrobacterium-mediated and particle bombardment |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/10164/ http://ir.unimas.my/id/eprint/10164/ http://ir.unimas.my/id/eprint/10164/ http://ir.unimas.my/id/eprint/10164/1/Genetic%20Transformation%20of%20Metroxylon%20sagu%20%28Rottb.%29%20Cultures%20via%20Agrobacterium-Mediated%20and%20Particle%20Bombardment%20%28abstract%29.pdf |