Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display
Chitinase is an enzyme that catalyzes the degradation of chitin, commonly induced upon the attack of pathogens and other stresses. A cDNA (MsChi1) was isolated from Metroxylon sagu and expressed predominantly in the inflorescence tissue of M. sagu, suggesting its role in developmental processes. The...
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| Format: | Article |
| Language: | English |
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Springer
2011
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| Online Access: | http://ir.unimas.my/id/eprint/10160/ http://ir.unimas.my/id/eprint/10160/1/Characterization%20of%20inflorescence-predominant%20chitinase%20gene%20in%20Metroxylon%20sagu%20via%20differential%20display%20%28abstract%29.pdf |
| _version_ | 1848836718625554432 |
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| author | Hairul Azman, Roslan Syahrul Bariyah, Anji |
| author_facet | Hairul Azman, Roslan Syahrul Bariyah, Anji |
| author_sort | Hairul Azman, Roslan |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Chitinase is an enzyme that catalyzes the degradation of chitin, commonly induced upon the attack of pathogens and other stresses. A cDNA (MsChi1) was isolated from Metroxylon sagu and expressed predominantly in the inflorescence tissue of M. sagu, suggesting its role in developmental processes. The chitinase cDNA was detected and isolated via differential display and rapid amplification of cDNA ends (RACE). Primers specific to M. saguchitinase were used as probes to amplify the 3′-end and 5′-end regions of chitinase cDNA. Transcript analysis showed that chitinase is expressed in inflorescence and meristem tissues but was not detected in the leaf tissue. Sequence analysis of amplified cDNA fragments of 3′-end and 5′-end regions indicated that the chitinase cDNA was successfully amplified. The M. saguchitinase cDNA isolated was approximately 1,143 bp long and corresponds to 312 predicted amino acids. Alignments of nucleotide and amino acid have grouped this chitinase to family 19 class I chitinase. |
| first_indexed | 2025-11-15T06:28:13Z |
| format | Article |
| id | unimas-10160 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:28:13Z |
| publishDate | 2011 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-101602016-01-13T00:33:43Z http://ir.unimas.my/id/eprint/10160/ Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display Hairul Azman, Roslan Syahrul Bariyah, Anji S Agriculture (General) SB Plant culture Chitinase is an enzyme that catalyzes the degradation of chitin, commonly induced upon the attack of pathogens and other stresses. A cDNA (MsChi1) was isolated from Metroxylon sagu and expressed predominantly in the inflorescence tissue of M. sagu, suggesting its role in developmental processes. The chitinase cDNA was detected and isolated via differential display and rapid amplification of cDNA ends (RACE). Primers specific to M. saguchitinase were used as probes to amplify the 3′-end and 5′-end regions of chitinase cDNA. Transcript analysis showed that chitinase is expressed in inflorescence and meristem tissues but was not detected in the leaf tissue. Sequence analysis of amplified cDNA fragments of 3′-end and 5′-end regions indicated that the chitinase cDNA was successfully amplified. The M. saguchitinase cDNA isolated was approximately 1,143 bp long and corresponds to 312 predicted amino acids. Alignments of nucleotide and amino acid have grouped this chitinase to family 19 class I chitinase. Springer 2011 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10160/1/Characterization%20of%20inflorescence-predominant%20chitinase%20gene%20in%20Metroxylon%20sagu%20via%20differential%20display%20%28abstract%29.pdf Hairul Azman, Roslan and Syahrul Bariyah, Anji (2011) Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display. 3 Biotech, 1 (1). pp. 27-33. ISSN 2190-5738 doi: 10.1007/s13205-011-0004-x |
| spellingShingle | S Agriculture (General) SB Plant culture Hairul Azman, Roslan Syahrul Bariyah, Anji Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display |
| title | Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display |
| title_full | Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display |
| title_fullStr | Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display |
| title_full_unstemmed | Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display |
| title_short | Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display |
| title_sort | characterization of inflorescence-predominant chitinase gene in metroxylon sagu via differential display |
| topic | S Agriculture (General) SB Plant culture |
| url | http://ir.unimas.my/id/eprint/10160/ http://ir.unimas.my/id/eprint/10160/ http://ir.unimas.my/id/eprint/10160/1/Characterization%20of%20inflorescence-predominant%20chitinase%20gene%20in%20Metroxylon%20sagu%20via%20differential%20display%20%28abstract%29.pdf |