Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak

Antibiotics have been widely used to control bacterial pathogens of aquaculture products. Aquaculture is becoming more economically important to increase local fish and shrimp production for food security. Thus, antimicrobial drugs are therefore used to tackle the disease problems and in some cases,...

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Main Author: Arweena, Iskandar
Format: Thesis
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2014
Subjects:
Online Access:http://ir.unimas.my/id/eprint/10154/
http://ir.unimas.my/id/eprint/10154/2/Arweena%20%20ft.pdf
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author Arweena, Iskandar
author_facet Arweena, Iskandar
author_sort Arweena, Iskandar
building UNIMAS Institutional Repository
collection Online Access
description Antibiotics have been widely used to control bacterial pathogens of aquaculture products. Aquaculture is becoming more economically important to increase local fish and shrimp production for food security. Thus, antimicrobial drugs are therefore used to tackle the disease problems and in some cases, they are used as growth promoter. However, prolong usage of the drugs may I ad to severe health problems to human as well as the resistance problem among the bacteria present The present study aims to detdermine the resistancy of the Enterobacteriaceae isolated from water and shrimp from the aquaculture environment. A total of 20 water samples and 40 shrimps were collected from 10 aquaculture pond in a farm in Kuala Baram, Miri, Sarawak. Isolates were first screened by using peR, followed by identification with API 20E Test Kit, and finally conducting the antimicrobial drugs resistant test. Four antimicrobial drugs were used in this study: ampicillin (AMP), chloramphenicol (C), ceftriaxone (eRO), and tetracycline (TE). The results reflected that the 31 bacterial isolates were classified into 4 different families: Enterobacteriaceae. Brucellaceae. Pseudomonadaceae; and Xanthomonadaceae. Antimicrobial resistance test rerevealed that 93.5% of the isolates were resistant to AMP, while others were susceptible to e, eRO and TE. This study revealed the presence of multiple antibiotic resistant bacteria from aquaculture environment.
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institution Universiti Malaysia Sarawak
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language English
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publisher Universiti Malaysia Sarawak, (UNIMAS)
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spelling unimas-101542025-05-13T06:40:15Z http://ir.unimas.my/id/eprint/10154/ Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak Arweena, Iskandar SH Aquaculture. Fisheries. Angling Antibiotics have been widely used to control bacterial pathogens of aquaculture products. Aquaculture is becoming more economically important to increase local fish and shrimp production for food security. Thus, antimicrobial drugs are therefore used to tackle the disease problems and in some cases, they are used as growth promoter. However, prolong usage of the drugs may I ad to severe health problems to human as well as the resistance problem among the bacteria present The present study aims to detdermine the resistancy of the Enterobacteriaceae isolated from water and shrimp from the aquaculture environment. A total of 20 water samples and 40 shrimps were collected from 10 aquaculture pond in a farm in Kuala Baram, Miri, Sarawak. Isolates were first screened by using peR, followed by identification with API 20E Test Kit, and finally conducting the antimicrobial drugs resistant test. Four antimicrobial drugs were used in this study: ampicillin (AMP), chloramphenicol (C), ceftriaxone (eRO), and tetracycline (TE). The results reflected that the 31 bacterial isolates were classified into 4 different families: Enterobacteriaceae. Brucellaceae. Pseudomonadaceae; and Xanthomonadaceae. Antimicrobial resistance test rerevealed that 93.5% of the isolates were resistant to AMP, while others were susceptible to e, eRO and TE. This study revealed the presence of multiple antibiotic resistant bacteria from aquaculture environment. Universiti Malaysia Sarawak, (UNIMAS) 2014 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/10154/2/Arweena%20%20ft.pdf Arweena, Iskandar (2014) Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak. Masters thesis, Universiti Malaysia Sarawak.
spellingShingle SH Aquaculture. Fisheries. Angling
Arweena, Iskandar
Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak
title Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak
title_full Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak
title_fullStr Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak
title_full_unstemmed Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak
title_short Isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in Kuala Baram, Miri, Sarawak
title_sort isolation, identification and antibiotic resistance of enterobactericeae from shrimp ponds in kuala baram, miri, sarawak
topic SH Aquaculture. Fisheries. Angling
url http://ir.unimas.my/id/eprint/10154/
http://ir.unimas.my/id/eprint/10154/2/Arweena%20%20ft.pdf