Rapid isolation and detection of Escherichia coli O157H7 by use of rainbow agar O157 and PCR assay
This study has evaluated the use of a commercially available Rainbow agar O157TM and polymerase chain reaction (PCR) assays for the detection of Shiga-like toxin producing Escherichia coli and to serotype E. coli O157:H7 from raw meat. The Rainbow agar O157TM was found to be selective and sensitiv...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
UPM
2000
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10109/ http://ir.unimas.my/id/eprint/10109/1/Radu.pdf |
| Summary: | This study has evaluated the use of a commercially available Rainbow agar O157TM and polymerase
chain reaction (PCR) assays for the detection of Shiga-like toxin producing Escherichia coli and to
serotype E. coli O157:H7 from raw meat. The Rainbow agar O157TM was found to be selective and sensitive
for the screening of the E. coli O157 from artificially and naturally contaminated meat samples. Shiga-like
toxin producing E. coli were identified with two primer pairs that amplified fragments of the SLT-I (384 bp)
and SLT-II (584 bp). E. coli O157:H7 was serotyped with a primer pair specified for the H7 flagellar gene,
which amplify specific DNA fragments (625 bp) from all E. coli O157:H7 strains. The use of Rainbow agar
O157TM described allows for the presumptive isolation of E. coli O157 in 24 hours. Identification and
confirmation of the presumptive isolates as E. coli O157:H7 by PCR assays require additional 6-8 hours.
The above-mentioned screening and identification procedures should prove to be a very useful method since
it allows for the specific detection of E. coli O157:H7. |
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