Isolation and molecular characterization of vancomycinresistant Enterococcus faecium in Malaysia
Nineteen strains of vancomycin-resistant Enterococcus faecium isolated from 10 of 75 (13·3%) tenderloin beef samples were examined for resistance to selected antibiotics, presence of plasmids, and genetic diversity by random amplification of polymorphic DNA analysis. All strains showed multiple...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The Society for Applied Microbiology
1999
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| Online Access: | http://ir.unimas.my/id/eprint/10102/ http://ir.unimas.my/id/eprint/10102/1/Nimita.pdf |
| _version_ | 1848836702822465536 |
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| author | Son, Radu Nimita, F. Rusul, G. Nasreldin, E. Samuel, Lihan Nishibuchi, M. |
| author_facet | Son, Radu Nimita, F. Rusul, G. Nasreldin, E. Samuel, Lihan Nishibuchi, M. |
| author_sort | Son, Radu |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Nineteen
strains of vancomycin-resistant Enterococcus faecium isolated from 10 of 75 (13·3%)
tenderloin beef samples were examined for resistance to selected antibiotics, presence
of plasmids, and genetic diversity by random amplification of polymorphic DNA
analysis. All strains showed multiple resistant to the antibiotics tested. Multiple
antibiotic indexing of the vancomycin-resistant E. faecium strains showed that all (100%)
originated from high risk contamination environments where antibiotics were often
used. Plasmids ranging in size from 1·5 to 36 megadalton were detected in 15 of 19 (79%)
strains. Thus, three plasmid profiles and eight antibiotypes were observed among the
E. faecium strains. A high degree of polymorphism was obtained by combining
the results of the two primers used; with the 19 E. faecium strains being differentiated
into 19 RAPD-types. These preliminary results suggest that RAPD-PCR has
application for epidemiologic studies and that resistance patterns and plasmid profiling
could be used as an adjunct to RAPD for the typing of E. faecium in the study
area. |
| first_indexed | 2025-11-15T06:27:58Z |
| format | Article |
| id | unimas-10102 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:27:58Z |
| publishDate | 1999 |
| publisher | The Society for Applied Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-101022021-07-14T17:10:42Z http://ir.unimas.my/id/eprint/10102/ Isolation and molecular characterization of vancomycinresistant Enterococcus faecium in Malaysia Son, Radu Nimita, F. Rusul, G. Nasreldin, E. Samuel, Lihan Nishibuchi, M. QR Microbiology Nineteen strains of vancomycin-resistant Enterococcus faecium isolated from 10 of 75 (13·3%) tenderloin beef samples were examined for resistance to selected antibiotics, presence of plasmids, and genetic diversity by random amplification of polymorphic DNA analysis. All strains showed multiple resistant to the antibiotics tested. Multiple antibiotic indexing of the vancomycin-resistant E. faecium strains showed that all (100%) originated from high risk contamination environments where antibiotics were often used. Plasmids ranging in size from 1·5 to 36 megadalton were detected in 15 of 19 (79%) strains. Thus, three plasmid profiles and eight antibiotypes were observed among the E. faecium strains. A high degree of polymorphism was obtained by combining the results of the two primers used; with the 19 E. faecium strains being differentiated into 19 RAPD-types. These preliminary results suggest that RAPD-PCR has application for epidemiologic studies and that resistance patterns and plasmid profiling could be used as an adjunct to RAPD for the typing of E. faecium in the study area. The Society for Applied Microbiology 1999 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/10102/1/Nimita.pdf Son, Radu and Nimita, F. and Rusul, G. and Nasreldin, E. and Samuel, Lihan and Nishibuchi, M. (1999) Isolation and molecular characterization of vancomycinresistant Enterococcus faecium in Malaysia. Letters in Applied Microbiology, 29. pp. 118-122. ISSN 0266-8254 |
| spellingShingle | QR Microbiology Son, Radu Nimita, F. Rusul, G. Nasreldin, E. Samuel, Lihan Nishibuchi, M. Isolation and molecular characterization of vancomycinresistant Enterococcus faecium in Malaysia |
| title | Isolation and molecular characterization of vancomycinresistant
Enterococcus faecium in Malaysia |
| title_full | Isolation and molecular characterization of vancomycinresistant
Enterococcus faecium in Malaysia |
| title_fullStr | Isolation and molecular characterization of vancomycinresistant
Enterococcus faecium in Malaysia |
| title_full_unstemmed | Isolation and molecular characterization of vancomycinresistant
Enterococcus faecium in Malaysia |
| title_short | Isolation and molecular characterization of vancomycinresistant
Enterococcus faecium in Malaysia |
| title_sort | isolation and molecular characterization of vancomycinresistant
enterococcus faecium in malaysia |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/10102/ http://ir.unimas.my/id/eprint/10102/1/Nimita.pdf |