Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates
Seven isolates of Burkholderia pseudomallei from cases of melioidosis in human (2 isolates) and animal (2 isolates), cat (one isolate) and from soil samples (2 isolates) were examined for in vitro sensitivity to 14 antimicrobial agents and for presence of plasmid DNA. Randomly amplified polymorphic...
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| Format: | Article |
| Language: | English |
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UPM
2000
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| Online Access: | http://ir.unimas.my/id/eprint/10101/ http://ir.unimas.my/id/eprint/10101/1/Samuel.pdf |
| _version_ | 1848836702548787200 |
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| author | Son, Radu Samuel, Lihan Azhar, Idris Oii, Wai Ling Muthafar Husein, Al-Haddawi Gulam, Rusul |
| author_facet | Son, Radu Samuel, Lihan Azhar, Idris Oii, Wai Ling Muthafar Husein, Al-Haddawi Gulam, Rusul |
| author_sort | Son, Radu |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Seven isolates of Burkholderia pseudomallei from cases of melioidosis in human (2 isolates) and animal (2 isolates), cat (one isolate) and from soil samples (2 isolates) were examined for in vitro sensitivity to 14 antimicrobial agents and for presence of plasmid DNA. Randomly amplified polymorphic DNA (RAPD) analysis was used to type the isolates, using two arbitrary primers. All isolates were sensitive to chloramphenicol, kanamycin, carbenicillin, rifampicin, enrofloxacin, tetracycline and sulfamethoxazole-trimethoprim. No plasmid was detected in all the isolates tested. RADP fingerprinting demonstrated genomic relationship between isolates, which provides an effective method to study the epidemiology of the isolates examined. |
| first_indexed | 2025-11-15T06:27:58Z |
| format | Article |
| id | unimas-10101 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:27:58Z |
| publishDate | 2000 |
| publisher | UPM |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-101012021-07-14T16:59:59Z http://ir.unimas.my/id/eprint/10101/ Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates Son, Radu Samuel, Lihan Azhar, Idris Oii, Wai Ling Muthafar Husein, Al-Haddawi Gulam, Rusul Q Science (General) Seven isolates of Burkholderia pseudomallei from cases of melioidosis in human (2 isolates) and animal (2 isolates), cat (one isolate) and from soil samples (2 isolates) were examined for in vitro sensitivity to 14 antimicrobial agents and for presence of plasmid DNA. Randomly amplified polymorphic DNA (RAPD) analysis was used to type the isolates, using two arbitrary primers. All isolates were sensitive to chloramphenicol, kanamycin, carbenicillin, rifampicin, enrofloxacin, tetracycline and sulfamethoxazole-trimethoprim. No plasmid was detected in all the isolates tested. RADP fingerprinting demonstrated genomic relationship between isolates, which provides an effective method to study the epidemiology of the isolates examined. UPM 2000 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/10101/1/Samuel.pdf Son, Radu and Samuel, Lihan and Azhar, Idris and Oii, Wai Ling and Muthafar Husein, Al-Haddawi and Gulam, Rusul (2000) Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates. The Southeast Asian journal of tropical medicine and public health, 30 (4). pp. 760-763. https://www.researchgate.net/publication/12392849_Genotypic_and_phenotypic_relationship_in_Burkholderia_pseudomallei_indicates_colonization_with_closely_related_isolates |
| spellingShingle | Q Science (General) Son, Radu Samuel, Lihan Azhar, Idris Oii, Wai Ling Muthafar Husein, Al-Haddawi Gulam, Rusul Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates |
| title | Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates |
| title_full | Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates |
| title_fullStr | Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates |
| title_full_unstemmed | Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates |
| title_short | Genotypic and phenotypic relationship in Burkholderia pseudomallei indicates colonization with closely related isolates |
| title_sort | genotypic and phenotypic relationship in burkholderia pseudomallei indicates colonization with closely related isolates |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/10101/ http://ir.unimas.my/id/eprint/10101/ http://ir.unimas.my/id/eprint/10101/1/Samuel.pdf |