Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles
A total of 70 isolates of Escherichia coli O157:H7 isolated from beef samples were examined with respect to plasmid profiles and random amplified polymorphic DNA (RAPD) patterns. All isolates carried the 90 kb pO157 plasmid alone or in combination with other smaller plasmids. Using Gen1-50-02 (5`-CA...
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| Format: | Article |
| Language: | English |
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Capricon Publication
2000
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| Online Access: | http://ir.unimas.my/id/eprint/10095/ http://ir.unimas.my/id/eprint/10095/1/Son.pdf |
| _version_ | 1848836701523279872 |
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| author | Son, Radu Rozila, Alias Gulam, Rusul Samuel, Lihan Ooi Wai, Ling |
| author_facet | Son, Radu Rozila, Alias Gulam, Rusul Samuel, Lihan Ooi Wai, Ling |
| author_sort | Son, Radu |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A total of 70 isolates of Escherichia coli O157:H7 isolated from beef samples were examined with respect to plasmid profiles and random amplified polymorphic DNA (RAPD) patterns. All isolates carried the 90 kb pO157 plasmid alone or in combination with other smaller plasmids. Using Gen1-50-02 (5`-CAATGCGTCT-3`), Gen1-50-09 (5`-AGAGGCGATG-3`) and Gen1-50-10 (5`-CCATTTACGC-3`) as primers, respectively, we obtained DNA polymorphisms which allowed us to discriminate the E. coli O157:H7 isolates into one, six and five RAPD patterns; providing bands ranging in size from 0.25 to 4.0 kb. Our results demonstrate that both plasmid profiling and RAPD-PCR fingerprinting methods are suitable tools for a fast and reliable molecular typing of E. coli O157:H7. The RAPD-PCR method is more sensitive with respect to the individualization of isolates and that RAPD-PCR assay could be a valuable technique for epidemiological studies. |
| first_indexed | 2025-11-15T06:27:57Z |
| format | Article |
| id | unimas-10095 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:27:57Z |
| publishDate | 2000 |
| publisher | Capricon Publication |
| recordtype | eprints |
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| spelling | unimas-100952021-07-14T16:56:01Z http://ir.unimas.my/id/eprint/10095/ Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles Son, Radu Rozila, Alias Gulam, Rusul Samuel, Lihan Ooi Wai, Ling Q Science (General) A total of 70 isolates of Escherichia coli O157:H7 isolated from beef samples were examined with respect to plasmid profiles and random amplified polymorphic DNA (RAPD) patterns. All isolates carried the 90 kb pO157 plasmid alone or in combination with other smaller plasmids. Using Gen1-50-02 (5`-CAATGCGTCT-3`), Gen1-50-09 (5`-AGAGGCGATG-3`) and Gen1-50-10 (5`-CCATTTACGC-3`) as primers, respectively, we obtained DNA polymorphisms which allowed us to discriminate the E. coli O157:H7 isolates into one, six and five RAPD patterns; providing bands ranging in size from 0.25 to 4.0 kb. Our results demonstrate that both plasmid profiling and RAPD-PCR fingerprinting methods are suitable tools for a fast and reliable molecular typing of E. coli O157:H7. The RAPD-PCR method is more sensitive with respect to the individualization of isolates and that RAPD-PCR assay could be a valuable technique for epidemiological studies. Capricon Publication 2000 Article PeerReviewed text en http://ir.unimas.my/id/eprint/10095/1/Son.pdf Son, Radu and Rozila, Alias and Gulam, Rusul and Samuel, Lihan and Ooi Wai, Ling (2000) Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles. Pakistan Journal of Biological Sciences, 3 (4). pp. 558-561. ISSN 1028-8880 https://www.researchgate.net/publication/26566743_Analysis_of_Escherichia_coli_O157H7_Isolates_from_Beef_by_RAPD-PCR_and_Plasmid_Profiles DOI: 10.3923/pjbs.2000.558.561 |
| spellingShingle | Q Science (General) Son, Radu Rozila, Alias Gulam, Rusul Samuel, Lihan Ooi Wai, Ling Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles |
| title | Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles |
| title_full | Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles |
| title_fullStr | Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles |
| title_full_unstemmed | Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles |
| title_short | Analysis of Escherichia coli O157:H7 Isolates from Beef by RAPD-PCR and Plasmid Profiles |
| title_sort | analysis of escherichia coli o157:h7 isolates from beef by rapd-pcr and plasmid profiles |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/10095/ http://ir.unimas.my/id/eprint/10095/ http://ir.unimas.my/id/eprint/10095/ http://ir.unimas.my/id/eprint/10095/1/Son.pdf |