Rnase Purification from Isolate Rns
Bacteria are the most preferred sources of RNase due to their broad biochemical diversity. The present study involved oOptimization and partial purification of RNase from a bacterial isolate from soil sample. Out of 12 bacterial isolates one isolate (RNS 3) was selected based on relatively higher RN...
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| Format: | Article |
| Language: | English |
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Penerbit UMP
2014
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| Online Access: | http://umpir.ump.edu.my/id/eprint/9524/ http://umpir.ump.edu.my/id/eprint/9524/1/6.pdf |
| _version_ | 1848818511401451520 |
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| author | Puranjan, Mishra Singh, Lakhveer Zularisam, A. W. |
| author_facet | Puranjan, Mishra Singh, Lakhveer Zularisam, A. W. |
| author_sort | Puranjan, Mishra |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Bacteria are the most preferred sources of RNase due to their broad biochemical diversity. The present study involved oOptimization and partial purification of RNase from a bacterial isolate from soil sample. Out of 12 bacterial isolates one isolate (RNS 3) was selected based on relatively higher RNase activity in the culture broth. The various physico-chemical parameters wereare consecutively optimized for maximum enzyme production. The optimized parameters are at were temperature of 34°C, incubation period (20 h) and inoculum size (10% v/v). Among different carbon and nitrogen sources supplementation of glucose and peptone, respectively to the broth showed enhanced RNase production. An alkaline pH (8.5) was suitable for maximum enzyme production by the bacterial isolate RNS3. |
| first_indexed | 2025-11-15T01:38:49Z |
| format | Article |
| id | ump-9524 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:38:49Z |
| publishDate | 2014 |
| publisher | Penerbit UMP |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-95242019-09-10T02:42:01Z http://umpir.ump.edu.my/id/eprint/9524/ Rnase Purification from Isolate Rns Puranjan, Mishra Singh, Lakhveer Zularisam, A. W. T Technology (General) Bacteria are the most preferred sources of RNase due to their broad biochemical diversity. The present study involved oOptimization and partial purification of RNase from a bacterial isolate from soil sample. Out of 12 bacterial isolates one isolate (RNS 3) was selected based on relatively higher RNase activity in the culture broth. The various physico-chemical parameters wereare consecutively optimized for maximum enzyme production. The optimized parameters are at were temperature of 34°C, incubation period (20 h) and inoculum size (10% v/v). Among different carbon and nitrogen sources supplementation of glucose and peptone, respectively to the broth showed enhanced RNase production. An alkaline pH (8.5) was suitable for maximum enzyme production by the bacterial isolate RNS3. Penerbit UMP 2014-06 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/9524/1/6.pdf Puranjan, Mishra and Singh, Lakhveer and Zularisam, A. W. (2014) Rnase Purification from Isolate Rns. International Journal of Engineering Technology & Sciences, 1 (1). pp. 2035-2037. ISSN 2289-697X. (Published) http://ijets.ump.edu.my/images/archive/Vol1/6.pdf |
| spellingShingle | T Technology (General) Puranjan, Mishra Singh, Lakhveer Zularisam, A. W. Rnase Purification from Isolate Rns |
| title | Rnase Purification from Isolate Rns |
| title_full | Rnase Purification from Isolate Rns |
| title_fullStr | Rnase Purification from Isolate Rns |
| title_full_unstemmed | Rnase Purification from Isolate Rns |
| title_short | Rnase Purification from Isolate Rns |
| title_sort | rnase purification from isolate rns |
| topic | T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/9524/ http://umpir.ump.edu.my/id/eprint/9524/ http://umpir.ump.edu.my/id/eprint/9524/1/6.pdf |