Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes...
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| Language: | English |
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Elsevier
2015
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| Online Access: | http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf |
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| author | Rohaida, Che Man Ahmad Fauzi, Ismail Nazlee Faisal, Ghazali Siti Fatimah Zaharah, Mohd Fuzi Rosli, Md. Illias |
| author_facet | Rohaida, Che Man Ahmad Fauzi, Ismail Nazlee Faisal, Ghazali Siti Fatimah Zaharah, Mohd Fuzi Rosli, Md. Illias |
| author_sort | Rohaida, Che Man |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes when using Escherichia coli as a host. In this study, the effects of different polymer of hollow fiber membrane and culture conditions on the enzyme excretion, cell lysis and plasmid stability of immobilized E. coli were investigated. The cells immobilized on a hollow fiber membrane composed of a polyvinylidene fluoride (PVDF) polymer exhibited a 2–4-fold increase in CGTase excretion, over a 100% increase in plasmid stability and 28-60% reduction in cell lysis compared with free cells. Hence, the immobilization of E. coli using a hollow fiber membrane was demonstrated to increase enzyme excretion and cell stability. |
| first_indexed | 2025-11-15T01:38:22Z |
| format | Article |
| id | ump-9404 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:38:22Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-94042017-10-30T01:58:54Z http://umpir.ump.edu.my/id/eprint/9404/ Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability Rohaida, Che Man Ahmad Fauzi, Ismail Nazlee Faisal, Ghazali Siti Fatimah Zaharah, Mohd Fuzi Rosli, Md. Illias TP Chemical technology The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification and more favorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes when using Escherichia coli as a host. In this study, the effects of different polymer of hollow fiber membrane and culture conditions on the enzyme excretion, cell lysis and plasmid stability of immobilized E. coli were investigated. The cells immobilized on a hollow fiber membrane composed of a polyvinylidene fluoride (PVDF) polymer exhibited a 2–4-fold increase in CGTase excretion, over a 100% increase in plasmid stability and 28-60% reduction in cell lysis compared with free cells. Hence, the immobilization of E. coli using a hollow fiber membrane was demonstrated to increase enzyme excretion and cell stability. Elsevier 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf Rohaida, Che Man and Ahmad Fauzi, Ismail and Nazlee Faisal, Ghazali and Siti Fatimah Zaharah, Mohd Fuzi and Rosli, Md. Illias (2015) Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability. Biochemical Engineering Journal, 98. pp. 91-98. ISSN 1369-703X. (Published) http://dx.doi.org/10.1016/j.bej.2015.02.013 doi: 10.1016/j.bej.2015.02.013 |
| spellingShingle | TP Chemical technology Rohaida, Che Man Ahmad Fauzi, Ismail Nazlee Faisal, Ghazali Siti Fatimah Zaharah, Mohd Fuzi Rosli, Md. Illias Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability |
| title | Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability |
| title_full | Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability |
| title_fullStr | Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability |
| title_full_unstemmed | Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability |
| title_short | Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability |
| title_sort | effects of the immobilization of recombinant escherichia coli on cyclodextrin glucanotransferase (cgtase) excretion and cell viability |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/ http://umpir.ump.edu.my/id/eprint/9404/1/fkksa-2015-rohaida-Effects%20of%20the%20Immobilization.pdf |