α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry
Tinospora crispa has been used in Malaysia to treat diabetes mellitus. We evaluated the effects of compounds isolated from T. crispa vines on the starch digestive enzymes, α-glucosidase and α-amylase. Borapetoside C was the most potent α-glucosidase inhibitor (IC50 = 0.0527 ± 0.008 mg/ml), followed...
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| Format: | Article |
| Language: | English English |
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Elsevier Ltd
2015
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| Online Access: | http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/1/fist-2015-mashitah- http://umpir.ump.edu.my/id/eprint/9262/2/a-Glucosidase%20and%20a-amylase%20Inhibitory%20constituents%20of%20Tinospora%20crispa-%20Isolation%20and%20Chemical%20Profile%20Confirmation%20by%20Ultra-High%20Performance%20Liquid%20Chromatography-Quadrupole%20Time-Of-Flight-Mass%20Spectrometry.pdf |
| _version_ | 1848818446262861824 |
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| author | Hazrulrizawati, Hamid M. M., Yusoff M., Liu M. R., Karim |
| author_facet | Hazrulrizawati, Hamid M. M., Yusoff M., Liu M. R., Karim |
| author_sort | Hazrulrizawati, Hamid |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Tinospora crispa has been used in Malaysia to treat diabetes mellitus. We evaluated the effects of compounds isolated from T. crispa vines on the starch digestive enzymes, α-glucosidase and α-amylase. Borapetoside C was the most potent α-glucosidase inhibitor (IC50 = 0.0527 ± 0.008 mg/ml), followed by 4-hydroxy benzaldehyde (IC50 = 0.557 ± 0.004 mg/ml), and the alkaloids lysicamine and liriodenine (IC50 = 0.562 ± 0.003 mg/ml). Borapetoside C also potently inhibited α-amylase inhibitory activity (IC50 = 0.775 ± 0.005 mg/ml). The relationship between compound activity and content in the extracts was determined by ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-QToF/MS). These results suggest that borapetoside C is the main primary active component of T. crispa extracts, mediating α-glucosidase and α-amylase inhibition. |
| first_indexed | 2025-11-15T01:37:47Z |
| format | Article |
| id | ump-9262 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T01:37:47Z |
| publishDate | 2015 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-92622018-07-26T07:53:06Z http://umpir.ump.edu.my/id/eprint/9262/ α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry Hazrulrizawati, Hamid M. M., Yusoff M., Liu M. R., Karim Q Science (General) Tinospora crispa has been used in Malaysia to treat diabetes mellitus. We evaluated the effects of compounds isolated from T. crispa vines on the starch digestive enzymes, α-glucosidase and α-amylase. Borapetoside C was the most potent α-glucosidase inhibitor (IC50 = 0.0527 ± 0.008 mg/ml), followed by 4-hydroxy benzaldehyde (IC50 = 0.557 ± 0.004 mg/ml), and the alkaloids lysicamine and liriodenine (IC50 = 0.562 ± 0.003 mg/ml). Borapetoside C also potently inhibited α-amylase inhibitory activity (IC50 = 0.775 ± 0.005 mg/ml). The relationship between compound activity and content in the extracts was determined by ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-QToF/MS). These results suggest that borapetoside C is the main primary active component of T. crispa extracts, mediating α-glucosidase and α-amylase inhibition. Elsevier Ltd 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9262/1/fist-2015-mashitah- application/pdf en http://umpir.ump.edu.my/id/eprint/9262/2/a-Glucosidase%20and%20a-amylase%20Inhibitory%20constituents%20of%20Tinospora%20crispa-%20Isolation%20and%20Chemical%20Profile%20Confirmation%20by%20Ultra-High%20Performance%20Liquid%20Chromatography-Quadrupole%20Time-Of-Flight-Mass%20Spectrometry.pdf Hazrulrizawati, Hamid and M. M., Yusoff and M., Liu and M. R., Karim (2015) α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry. Journal of Functional Foods, 16. pp. 74-80. ISSN 1756-4646. (Published) http://dx.doi.org/10.1016/j.jff.2015.04.011 doi: 10.1016/j.jff.2015.04.011 |
| spellingShingle | Q Science (General) Hazrulrizawati, Hamid M. M., Yusoff M., Liu M. R., Karim α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry |
| title | α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry |
| title_full | α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry |
| title_fullStr | α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry |
| title_full_unstemmed | α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry |
| title_short | α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry |
| title_sort | α-glucosidase and α-amylase inhibitory constituents of tinospora crispa: isolation and chemical profile confirmation by ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/1/fist-2015-mashitah- http://umpir.ump.edu.my/id/eprint/9262/2/a-Glucosidase%20and%20a-amylase%20Inhibitory%20constituents%20of%20Tinospora%20crispa-%20Isolation%20and%20Chemical%20Profile%20Confirmation%20by%20Ultra-High%20Performance%20Liquid%20Chromatography-Quadrupole%20Time-Of-Flight-Mass%20Spectrometry.pdf |