Effect of imidazole concentration and type of metal ion on the purification of recombinant green fluorescent protein using an affinity chromatography

QR Code

Effect of imidazole concentration and type of metal ion on the purification of recombinant green fluorescent protein using an affinity chromatography

The purification of protein using IMAC can be influenced by imidazole concentration and type of metal ion. Different type of metal ion and different imidazole concentration will give different result of purity and yield for target protein. The strength of binding between histidine- tagged protein an...

Full description

Bibliographic Details
Main Author:	Insyirah, Samsuddin
Format:	Undergraduates Project Papers
Language:	English
Published:	2014
Subjects:	QP Physiology
Online Access:	http://umpir.ump.edu.my/id/eprint/8853/ http://umpir.ump.edu.my/id/eprint/8853/1/CD8547.pdf

Similar Items

Electrophoretic purification of recombinant green fluorescent protein from intact escherichia coli cells in continuous buffer system
by: Chau, King Hou
Published: (2014)

The effect of buffers ph on electrophoretic purification of intracellular green fluorescent protein
by: Hazlin, Mohamed
Published: (2014)

Preliminary study on parameters of lysozyme purification using ion exchange chromatography
by: Wan Norlinda Roshana, Mohd Nawi
Published: (2006)

Effect of irradiation of ultraviolet on the quantitation method of enhanced green fluorescent protein
by: Chee Heng, Hor
Published: (2015)

Effect of growth conditions on production of green fluorescent protein from escherichia coli fermentation
by: Malini, Subramaniam
Published: (2014)

Stability of papain in aqueous organic solvent by reverse phase liquid chromatography
by: Muhammad Yunus, Zainal Abidin
Published: (2013)

Optimization of inoculum size & agitation rate on expression of recombinant chitinase in Escherichia coli
by: Nor Fadzillah Hanis, Abu Sulaiman
Published: (2009)

Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity
by: Chan, Mooi Kwai
Published: (2017)

Protein-protein interactions during Japanese encephalitis infection
by: Magdline Sia, Henry Sum.
Published: (2007)

Determination of glufosinate-ammonium and malathion in residue oil from palm pressed fiber using high performance liquid chromatography
by: Muhammad Haziq, Russli
Published: (2012)

hermostability of protein in starch derivative emulsion
by: Siti Suhairah, Omar
Published: (2013)

Studying the effect of chitosan as drag reducing agent in water flowing systems with different concentration and preparation using different acid types
by: Nur Khadijah, Mohamad Najib
Published: (2009)

Effect of substrate concentration on profile growth of candida tropicalis ifo 0618
by: Siti Nur Hatika, Abu Bakar
Published: (2014)

Formulation of Wound Healing Hydrogel from Keratin Protein
by: Rabiatul Adawiyah, Zaukifli
Published: (2012)

Production of Beta-Carotene by Dunaliella Salina : Effect of Nano3 Concentration and Light Intensity
by: Nur Isma Idayu, Ismail
Published: (2012)

Studying the effect addition of chitosan and suspended solid as drag reducing agent with different value of concentrations in turbulent water flowing system
by: Noriman Hamizan, Hamdan
Published: (2009)

Filamin A-interacting protein (FILIP) is a region-specific modulator of myosin 2b and controls spine morphology and NMDA receptor accumulation
by: Yagi, Hideshi, et al.
Published: (2014)

Proteomic Analysis of Differentially Expressed Protein in Hemocytes of Wild Giant Freshwater Prawn Macrobrachium Rosenbergii Infected with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV)
by: Alinejad, T., et al.
Published: (2015)

Production and electrophoresis-based purification of recombinant green fluorescent protein from Escherichia coli
by: Chew, Few Ne
Published: (2011)

Expression and purification of hemagglutinin and neuraminidase recombinant protein of avian influenza a virus using immobilised metal affinity chromatography (imac)
by: Chiang, Goh Wei
Published: (2016)

Direct recovery of papain from papaya juice using expanded bed adsorption chromatography
by: He, Yu Hai
Published: (2013)

Design, Synthesis and Docking Studies of Flavokawain B Type Chalcones and Their Cytotoxic Effects on MCF-7 and MDA-MB-231 Cell Lines
by: Addila, Abu Bakar, et al.
Published: (2018)

Protein purification by using immobilized metal ions affinity zeolite adsorbent
by: Su Najwa, Mohamed
Published: (2010)

Protein purification by using immobilized metal ion affinity (IMA) adsorbent
by: Fidelia Sawai, anak Michael Mulok
Published: (2009)

The effect of lead exposure of mice during pregnancy on the concentration and motility of epididymal and testicular spermatozoa in offspring mature male mice
by: Al-Khfaji, Ina'am, et al.
Published: (2011)

Hubungan kecergasan fizikal, kecerdasan emosi dan kecenderungan seksual dalam kalangan remaja perempuan berumur 17 tahun. / Rohana bt Yahya
by: Rohana, Yahya
Published: (2015)

The relationship between anthropometry and hand grip strength among older Malaysian people / Nurul Shahida Mohd Shalahim
by: Nurul Shahida, Mohd Shalahim
Published: (2016)

Illustrated human physiology
by: Mohd Noor, Noriah
Published: (2015)

Effects of high and low maternal dietary sodium intake during pregnancy on the offsprings’ glomerular number in rats
by: Alias, Nurul Adilah, et al.
Published: (2015)

Apolipoprotein e gene polymorphisms in essential hypertension: a preliminary study with meta-analysis
by: Ibrahim, Wisam Nabeel, et al.
Published: (2015)

Cartilage cell and tissue based therapy for focal defect and traumatic osteoarthritis of the articular cartilage in the knee joint
by: BHI, Ruzymah, et al.
Published: (2009)

A study on angiotensin converting enzyme gene insertion/ deletion polymorphism in cardiovascular diseases
by: Ibrahim, Wisam Nabeel, et al.
Published: (2014)

Nitric oxide synthase Glu298Asp gene polymorphism in cardiovascular diseases
by: Ibrahim, Wisam Nabeel, et al.
Published: (2014)

Apolipoprotein E gene polymorphism in hypertension
by: Ibrahim, Wisam Nabeel, et al.
Published: (2013)

The use of pulmonary function test to predict occupational lung disease among workers in steel industry
by: Ibrahim, Wisam Nabeel, et al.
Published: (2013)

In vivo ctcf and yb-1 interaction with ap-1 site of matrix metalloproteinase 13 gene promoter in human malignant melanoma cell line
by: Ibrahim, Wisam Nabeel, et al.
Published: (2014)

Anti-inflammatory activity of kapok (ceiba pentandra) thorns in acute inflammation
by: Hashim, Ridzwan, et al.
Published: (2014)

Toxicity effects of water extracts of Holothuria atra Jaeger in mice
by: Hashim, Ridzwan, et al.
Published: (2014)

2VO as affordable model for neurodegeneration- IIUM experience
by: Allow, Ahmed Kaid Naji
Published: (2018)

A secondary research on human endocrine physiology in Al- Qur’an and Hadith
by: Abdul Ghani, Radiah, et al.
Published: (2017)