Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection
There are various methods to separate between macro molecule and non dissolved particle in chemical process. One of the methods is separation process using ultrafiltarion membrane. In filtration process, the macromolecules such as enzyme will be retained on the membrane surface. This experiment is s...
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/883/ http://umpir.ump.edu.my/id/eprint/883/1/Mohd_Khairul_Afizan_Harun.pdf |
| _version_ | 1848816734101831680 |
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| author | Mohd Khairul Afizan, Harun |
| author_facet | Mohd Khairul Afizan, Harun |
| author_sort | Mohd Khairul Afizan, Harun |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | There are various methods to separate between macro molecule and non dissolved particle in chemical process. One of the methods is separation process using ultrafiltarion membrane. In filtration process, the macromolecules such as enzyme will be retained on the membrane surface. This experiment is study about fouling characteristic occur in an industry. The fouled membrane surface problem gives the high cost operation and reduces the quality of production. Therefore, the main objectives for this experiment are to determine the effect of pH and ionic strength on membrane flux and rejection during fructosyltransferase (FTase) separation. The 50 kDa molecular weight cut off (MWCO) of ultrafiltration membrane was used during this experiment. Cross flow filtration was used to run this experiment in the lab scale. Total organic carbon (TOC) was used to analysis the concentration of sample. Potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate buffer solution range pH 5 to pH 8 was applied to find the effect of pH and various molarities of NaCl (0.5M to 2.0M) was used to find the effect of ionic strength in ultrafiltration membrane. The experimental result shows that the optimum pH and ionic strength was 8.0 and 0.5M, respectively, in order to separate the FTase solution using ultrafiltration membrane. |
| first_indexed | 2025-11-15T01:10:34Z |
| format | Undergraduates Project Papers |
| id | ump-883 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:10:34Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-8832021-06-17T07:05:12Z http://umpir.ump.edu.my/id/eprint/883/ Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection Mohd Khairul Afizan, Harun TP Chemical technology There are various methods to separate between macro molecule and non dissolved particle in chemical process. One of the methods is separation process using ultrafiltarion membrane. In filtration process, the macromolecules such as enzyme will be retained on the membrane surface. This experiment is study about fouling characteristic occur in an industry. The fouled membrane surface problem gives the high cost operation and reduces the quality of production. Therefore, the main objectives for this experiment are to determine the effect of pH and ionic strength on membrane flux and rejection during fructosyltransferase (FTase) separation. The 50 kDa molecular weight cut off (MWCO) of ultrafiltration membrane was used during this experiment. Cross flow filtration was used to run this experiment in the lab scale. Total organic carbon (TOC) was used to analysis the concentration of sample. Potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate buffer solution range pH 5 to pH 8 was applied to find the effect of pH and various molarities of NaCl (0.5M to 2.0M) was used to find the effect of ionic strength in ultrafiltration membrane. The experimental result shows that the optimum pH and ionic strength was 8.0 and 0.5M, respectively, in order to separate the FTase solution using ultrafiltration membrane. 2009-04 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/883/1/Mohd_Khairul_Afizan_Harun.pdf Mohd Khairul Afizan, Harun (2009) Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. |
| spellingShingle | TP Chemical technology Mohd Khairul Afizan, Harun Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection |
| title | Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection |
| title_full | Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection |
| title_fullStr | Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection |
| title_full_unstemmed | Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection |
| title_short | Separation of fructosyltransferase using ultrafiltration membrane : effect of pH and ionic strength on flux and rejection |
| title_sort | separation of fructosyltransferase using ultrafiltration membrane : effect of ph and ionic strength on flux and rejection |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/883/ http://umpir.ump.edu.my/id/eprint/883/1/Mohd_Khairul_Afizan_Harun.pdf |